1887

Abstract

Leaf and stem explants of cv. Pusa Bold produced callus tissue and plantlets on Murashige and Skoog medium at 18°C. Among the various organs of inflorescences, systemically infected with explants from hypertrophied peduncles and thickened terminal leaves proved suitable for the production of dua cultures of the biotroph and its host, success with the former being better than with the latter. Dual cultures were also established from ovaries, but poorly. Infected callus grew faster than its healthy counterpart. Leaf and sten pieces with rust pustules did not form callus. The dual culture showed abundant coenocytic intercellular mycelium with spherical haustoria within host cells. Infected callus maintained at 15 and 20 °C for 20 to 30 d showed only sporangial chains that remained confined within the callus tissue, whereas callus maintained at 25 and 30 °C contained both sporangia and oospores, like those of found in naturally infected tissues. The pathoge could be maintained in infected callus tissue for prolonged periods by periodic subculturing and it kept pace with the growth of the callus tissue. Placement of an infected callus in close contact with a healthy one did no result in infection of the latter.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-11-2875
1993-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/11/mic-139-11-2875.html?itemId=/content/journal/micro/10.1099/00221287-139-11-2875&mimeType=html&fmt=ahah

References

  1. Bains S. S., Jhooty J. S. 1979; Mixed infections by Albugo Candida and Peronospora parasitica on Brassica juncea inflorescence and their control. Indian Phytopathology 32:268–271
    [Google Scholar]
  2. Cutter V. M. 1960; Studies on the isolation and growth of plant rusts in plant tissue cultures and upon synthetic media. II. Uromyces ari-triphylli. Mycologia 52:726–742
    [Google Scholar]
  3. Griffin M. J., Coley-Smith J. R. 1968; The establishment of hop tissue cultures and their infection by downy mildew Pseudoperonospora humuli (Miy. & Tak.) Wilson under aseptic conditions. Journal of General Microbiology 53:231–236
    [Google Scholar]
  4. Harvey A. E., Grasham J. L. 1971; Inoculation of a non-host tissue culture with Cronartium ribicola. Canadian Journal of Botany 49:881–882
    [Google Scholar]
  5. Hirata S. 1954; Studies on the phytohormone in malformed portion of the diseased plants. I. The relation between the growth rate and the amount of free auxin in fungus galls and virus infected plants. Annals of the Phytopathological Society of Japan 19:33–38
    [Google Scholar]
  6. Hotson H. H., Cutter V. M. 1951; The isolation and culture of Gymnosporangium juniperi-viriginianae. Proceedings of the National Academy of Sciences of the United States of America 37400–403
    [Google Scholar]
  7. Ingram D. S. 1969; Growth of Plasmodiophora brassicae in host callus. Journal of General Microbiology 55:9–18
    [Google Scholar]
  8. Johansen D. A. 1940 Plant Microtechnique New York: McGraw Hill;
    [Google Scholar]
  9. Kiermayer O. 1958; Paper chromatographic studies of the growth substances of Capsella bursa-pastoris after infection by Albugo Candida and Peronospora parasitica. Österreichische Botanische Zeitschrift 105:515–528
    [Google Scholar]
  10. Morel G. 1944; Le développement du mildiou sur des tissus de vigne cultivés in vitro. Comptes Rendus Hebdomadaires des Séances de l’Academie des Sciences, Paris 218:50–52
    [Google Scholar]
  11. Murashige T., Skoog F. 1962; A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473–497
    [Google Scholar]
  12. Singh H. 1963; The growth of Albugo in the callus culture of Ipomoea. Current Science 10:472–473
    [Google Scholar]
  13. Strandberg J. O., Williams P. H., Yukawa Y. 1966; Monoaxenic culture of Plasmodiophora brassicae with cabbage tissue. Phytopathology 56:903 abstract
    [Google Scholar]
  14. Tiwari M. H., Arya H. C. 1969; Sclerospora graminicola axenic culture. Science 163:291–293
    [Google Scholar]
  15. Verma U., Bhowmik T. P. 1986; A simple method of inoculating white rust on rapeseed and mustard. International Journal of Tropical Plant Disease 4:41–43
    [Google Scholar]
  16. Verma U., Bhowmik T. P. 1989; Inheritance of resistance to a Brassica juncea pathotype of Albugo Candida in B. napus. Canadian Journal of Plant Pathology 11:443–444
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-11-2875
Loading
/content/journal/micro/10.1099/00221287-139-11-2875
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error