Skip to content
1887

Microbiology Society logo Microbiology

Volume 139, Issue 11

Diversity of environmental isolates showing larvicidal activity specific for mosquitoes Free

Abstract

Seven mosquito-specific strains of isolated in Japan were examined for their flagellar(H)antigenicities and the properties of parasporal inclusion proteins. They were assigned to six H serovars: serovar sis (H 3ade); serovar (H 5ac); serovar (H 10); serovar (H): serovar (H 22); and an undescribed serovar belonging to H serotype 20. Purified parasporal inclusions exhibited moderate mosquito larvicidal activities with LC values ranging from 1 μg ml to 10 μg ml. The inclusions of these strains consisted of highly heterogeneous multiple protein components, and five distinct patterns were evident in their SDS-PAGE profiles. Antibodies against inclusion proteins were reactive with all strains to varying degrees, while antibodies gave only relatively weak reactions with the seven strains. Similarity in antibody-binding profiles was associated with similarity in SDS-PAGE profiles. In a given strain, different antisera gave altered immunoblot profiles. Haemolytic activity was shown by solubilized parsporal inclusion proteins of five of the seven strains.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1993
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-11-2849
1993-11-01
2025-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/11/mic-139-11-2849.html?itemId=/content/journal/micro/10.1099/00221287-139-11-2849&mimeType=html&fmt=ahah

References

  1. Abdel-Hameed A., Carlberg G., El-Tayeb O. M. 1990; Studies on Bacillus thuringiensis H-14 strains isolated in Egypt. I. Screening for active strains. World Journal of Microbiology and Biotechnology 6:299–304
     [Google Scholar]
  2. Balaraman K., Hoti S. L., Manonmani L. M. 1981; An indigenous virulent strain of Bacillus thuringiensis, highly pathogenic and specific to mosquitoes. Current Science 50:199–200
     [Google Scholar]
  3. Cheung T.-Y., Kim K.-H. 1990; Immunological characteristics of mosquitocidal delta-endotoxin from Bacillus thuringiensis subsp.darmstadiensis 73E10–2. Korean Journal of Applied Microbiology and Bioengineering 18:301–304
     [Google Scholar]
  4. De Barjac H. 1978; Une nouvelle variété de Bacillus thuringiensis trés toxique pour les moustiques: B. thuringiensis var.israelensis sérotype 14. Comptes Rendus de l’Académie des Sciences, Série D 286:797–800
     [Google Scholar]
  5. De Barjac H., Sutherland D. J. 1990 Bacterial Control of Mosquitoes and Black Flies London: Unwin Hyman;
     [Google Scholar]
  6. Drobniewski F. A., Ellar D. J. 1989; Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73–E10–2. Journal of Bacteriology 171:3060–3067
     [Google Scholar]
  7. Earp D. J., Ward E. S., Ellar D. J. 1987; Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis. FEMS Microbiology Letters 42:195–199
     [Google Scholar]
  8. Finney D. J. 1952 Probit Analysis, 2nd edn.. London: Cambridge University Press;
     [Google Scholar]
  9. Gill S. S., Hornung J. M. 1987; Cytolytic activity of Bacillus thuringiensis proteins to insect and mammalian cell lines. Journal of Invertebrate Pathology 50:16–25
     [Google Scholar]
  10. Goldberg L. J., Margalit J. 1977; A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univittatus, Aedes aegypti and Culex pipiens. Mosquito News 37:355–358
     [Google Scholar]
  11. Goodman N. S., Gottfried R. J., Rogoff M. H. 1967; Biphasic system for separation of spores and crystals of Bacillus thuringiensis. Journal of Bacteriology 94:485
     [Google Scholar]
  12. Held G. A., Kawanishi C. Y., Huang Y.-S. 1990; Characterization of the parasporal inclusion of Bacillus thuringiensis subsp. kyushuensis. Journal of Bacteriology 172:481–483
     [Google Scholar]
  13. Höfte H., Whiteley H. R. 1989; Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews 53:242–255
     [Google Scholar]
  14. Ishii T., Ohba M. 1992; Immunological relationships between parasporal inclusions of Bacillus thuringiensis serovar kyushuensisand the three other mosquito-specific Bacillus thuringiensis strains. Journal of General and Applied Microbiology 38:385–389
     [Google Scholar]
  15. Kim K.-H., Ohba M., Aizawa K. 1984; Purification of the toxic protein from Bacillus thuringiensis serotype 10 isolate demonstrating a preferential larvicidal activity to the mosquito. Journal of Invertebrate Pathology 44:214–219
     [Google Scholar]
  16. Knowles B. H., White P. J., Nicholls C. N., Ellar D. J. 1992; A broad-spectrum cytolytic toxin from Bacillus thuringiensis var. kyushuensis. Proceedings of the Royal Society of London B2481–7
     [Google Scholar]
  17. Krieg A., Schnetter W., Huger A. L., Langenbruch G. A. 1987; Bacillus thuringiensis subsp.tenebrionis, strain BI 256–82: a third pathotype within the H serotype 8a8b. Systematic and Applied Microbiology 9:138–141
     [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
     [Google Scholar]
  19. Ohba M., Aizawa K. 1978; Serological identification of Bacillus thuringiensis and related bacteria isolated in Japan. Journal of Invertebrate Pathology 32:303–309
     [Google Scholar]
  20. Ohba M., Aizawa K. 1979; A new subspecies of Bacillus thuringiensis possessing 11a: 11c flagellar antigenic structure: Bacillus thuringiensis subsp. kyushuensis. Journal of Invertebrate Pathology 33:387–388
     [Google Scholar]
  21. Ohba M., Aizawa K. 1986a; Insect toxicity of Bacillus thuringiensis isolated from soils of Japan. Journal of Invertebrate Pathology 47:12–20
     [Google Scholar]
  22. Ohba M., Aizawa K. 1986b; Distribution of Bacillus thuringiensis in soils of Japan. Journal of Invertebrate Pathology 47:277–282
     [Google Scholar]
  23. Ohba M., Aizawa K. 1990; Occurrence of two pathotypes in Bacillus thuringiensis subsp. fukuokaensis (flagellar serotype 3a:3d:3e). Journal of Invertebrate Pathology 55:293–294
     [Google Scholar]
  24. Orduz S., Rojas W., Correa M. M., Montoya A. E., De Barjac H. 1992; A new serotype of Bacillus thuringiensis from Colombia toxic to mosquito larvae. Journal of Invertebrate Pathology 59:99–103
     [Google Scholar]
  25. Padua L. E., Ohba M., Aizawa K. 1980; The isolates of Bacillus thuringiensis serotype 10 with a highly preferential toxicity to mosquito larvae. Journal of Invertebrate Pathology 36:180–186
     [Google Scholar]
  26. Padua L. E., Ohba M., Aizawa K. 1984; Isolation of a Bacillus thuringiensis strain (serotype 8a:8b) highly and selectively toxic against mosquito larvae. Journal of Invertebrate Pathology 44:12–17
     [Google Scholar]
  27. Padua L. E., Ohba M., Aizawa K. 1988; Comparative serology of crystals produced by PG-14, Bacillus thuringiensis subsp. morrisoni (serotype H 8a :8b) and Bacillus thuringiensis subsp. israelensis(serotype H 14). Journal of Invertebrate Pathology 52:192–194
     [Google Scholar]
  28. Pietrantonio P. V., Gill S. S. 1992; The parasporal inclusion of Bacillus thuringiensis subsp.shandongiensis: characterization and screening for insecticidal activity. Journal of Invertebrate Pathology 59:295–302
     [Google Scholar]
  29. Shim J. C., Yoon Y. H., Yeon K. N., Shim S. B., Yu H. S. 1990; Isolation of Bacillus thuringiensis from soil and control effect of medically important insects. Korean Journal of Entomology 20:179–188
     [Google Scholar]
  30. Thomas W. E., Ellar D. J. 1983; Bacillus thuringiensis var. israelensis crystal δ-endotoxin: effects on insect and mammalian cells in vitro and in vivo. Journal of Cell Science 60:181–197
     [Google Scholar]
  31. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
     [Google Scholar]
  32. Wang Y., Wen J., Feng X. 1986; A new serovar of Bacillus thuringiensis. Acta Microbiologica Sinica 26:1–6
     [Google Scholar]
  33. Wu D., Chang F. N. 1985; Synergism in mosquitocidal activity of 26 and 65 kDa proteins from Bacillus thuringiensis subsp. israelensis crystal. FEBS Letters 190:232–236
     [Google Scholar]
  34. Yu Y. M., Ohba M., Aizawa K. 1987; Synergistic effects of the 65- and 25-kilodalton proteins of Bacillus thuringiensis strain PG-14 (serotype 8a:8b) in mosquito larvicidal activity. Journal of General and Applied Microbiology 33:459–462
     [Google Scholar]
  35. Yu Y. M., Ohba M., Gill S. S. 1991; Characterization of mosquitocidal activity of Bacillus thuringiensis subsp. fukuokaensis crystal proteins. Applied and Environmental Microbiology 57:1075–1081
     [Google Scholar]
  36. Zhang Y., Ku Z., Chen Z., Xu B., Yuan F., Chen G., Zhong T., Ming G. 1984; A new isolate of Bacillus thuringiensis possessing high toxicity against the mosquitoes. Acta Microbiologica Sinica 24:320–325
     [Google Scholar]
/content/journal/micro/10.1099/00221287-139-11-2849
Loading
Diversity of Bacillus thuringiensis environmental isolates showing larvicidal activity specific for mosquitoes
Microbiology 139, 2849 (1993); https://doi.org/10.1099/00221287-139-11-2849
/content/journal/micro/10.1099/00221287-139-11-2849
/content/journal/micro/10.1099/00221287-139-11-2849
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error