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Volume 139, Issue 11

Diversity of environmental isolates showing larvicidal activity specific for mosquitoes Free

Abstract

Seven mosquito-specific strains of isolated in Japan were examined for their flagellar(H)antigenicities and the properties of parasporal inclusion proteins. They were assigned to six H serovars: serovar sis (H 3ade); serovar (H 5ac); serovar (H 10); serovar (H): serovar (H 22); and an undescribed serovar belonging to H serotype 20. Purified parasporal inclusions exhibited moderate mosquito larvicidal activities with LC values ranging from 1 μg ml to 10 μg ml. The inclusions of these strains consisted of highly heterogeneous multiple protein components, and five distinct patterns were evident in their SDS-PAGE profiles. Antibodies against inclusion proteins were reactive with all strains to varying degrees, while antibodies gave only relatively weak reactions with the seven strains. Similarity in antibody-binding profiles was associated with similarity in SDS-PAGE profiles. In a given strain, different antisera gave altered immunoblot profiles. Haemolytic activity was shown by solubilized parsporal inclusion proteins of five of the seven strains.

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© Society for General Microbiology 1993
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1993-11-01
2024-04-23
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Diversity of Bacillus thuringiensis environmental isolates showing larvicidal activity specific for mosquitoes
Microbiology 139, 2849 (1993); https://doi.org/10.1099/00221287-139-11-2849
/content/journal/micro/10.1099/00221287-139-11-2849
/content/journal/micro/10.1099/00221287-139-11-2849
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