1887

Abstract

The effects of UV irradiation on infection of nematodes by two nematode-trapping fungi were studied. Five minutes UV irradiation of the fungi caused an inhibition of the infection process in both and After the nematodes were captured, the infection process stopped; the nematodes were still moving at a time when the nematodes in untreated controls were completely digested. The UV-irradiated fungi were still alive and were capable of further growth. After 15 min UV irradiation the number of captured nematodes was also reduced. Inhibition studies with cycloheximide showed differences between the two fungi. The nematodes were still alive 24 h after capture by treated cultures of while the infection process was normally completed by There were minor differences in the peptide pattern on a SDS-PAGE gel between proteins from untreated and UV-irradiated mycelia of both fungi. Both the results from inhibition experiments and changes in the peptide pattern after UV irradiation suggest that the target molecules affected by UV irradiation may have been proteins involved in the infection process, and not the DNA. No effect of UV irradiation was observed on molecules that had been suggested previously to be involved in the nematode infection process (toxins, lectins and proteases).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-11-2841
1993-11-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/11/mic-139-11-2841.html?itemId=/content/journal/micro/10.1099/00221287-139-11-2841&mimeType=html&fmt=ahah

References

  1. Bourret J. A. 1987; The mechanism by which 2-deoxyglucose inhibits glucose-induced activation of Pilobolus longipes spores. Experimental Mycology 11:307–316
    [Google Scholar]
  2. Biely P., Krátky Z., Kovírik J., Bauer S. 1971; Effect of 2-deoxyglucose on cell wall formation in Saccharomyces cerevisiae and its relation to cell growth inhibition. Journal of Bacteriology 107:121–129
    [Google Scholar]
  3. Chavira R. Jr Burnett T. J., Hageman J. H. 1984; Assaying proteinases with Azocoll. Analytical Biochemistry 136:446–450
    [Google Scholar]
  4. Dowsett J. A., Reid J. 1977a; Light microscope observations on the trapping of nematodes by Dactylaria candida. Canadian Journal of Botany 55:2956–2962
    [Google Scholar]
  5. Dowsett J. A., Reid J. 1977b; Transmission and scanning electron microscope observations on the trapping of nematodes by Dactylaria candida. Canadian Journal of Botany 55:2963–2970
    [Google Scholar]
  6. Friman E., Olsson S., Nordbring-Hertz B. 1985; Heavy trap formation by Arthrobotrys oligospora in liquid culture. FEMS Microbiology Ecology 31:17–21
    [Google Scholar]
  7. Gurr E. 1960 Encyclopedia of Microscopic Stains pp. 213–214 Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Mckay D. B., Maclean D. J. 1992; Effect of glucose and glucitol analogues on respiration by mycelia of Puccinia graminis. Experimental Mycology 16:110–118
    [Google Scholar]
  9. Murray D. S., Wharton D. A. 1990; Capture and penetration processes of the free-living juveniles of Trichostrongylus colubriformis (Nematoda) by the nematophagous fungus, Arthrobotrys oligospora. Parasitology 101:93–100
    [Google Scholar]
  10. Nordbring-Hertz B. 1973; Peptide-induced morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. Physiologia Plantarum 29:223–233
    [Google Scholar]
  11. Nordbring-Hertz B. 1983; Dialysis membrane technique for studying microbial interaction. Applied and Environmental Microbiology 45:290–293
    [Google Scholar]
  12. Nordbring-Hertz B., Mattiasson B. 1979; Action of a nematode-trapping fungus shows lectin-mediated host-micro-organism interaction. Nature; London: 281477–479
    [Google Scholar]
  13. Nordbring-Hertz B., Friman E., Mattiasson B. 1982; A recognition mechanism in the adhesion of nematodes to nematode-trapping fungi. In Lectins - Biology, Biochemistry, Clinical Biochemistry II pp. 83–90 Bog-Hansen T. C. Edited by Berlin: Walter de Gruyter;
    [Google Scholar]
  14. Nordbring-Hertz B., Jansson H.-B., Friman E., Persson Y., Dackman C., Hard T., Poloczek E. 1993; Mechanisms of capture in nematophagous fungi. Film V 2603. IWF; Göttingen, Germany:
    [Google Scholar]
  15. Olthof H. A., Estey R. H. 1963; A nematotoxin produced by the nematophagous fungus Arthrobotrys oligospora Fresenius. Nature; London: 197514–515
    [Google Scholar]
  16. Persson Y., Friman E. 1993; Intracellular proteolytic activity in mycelia of Arthrobotrys oligospora bearing mycoparasitic or nematode-trapping structures. Experimental Mycology 17: in the Press
    [Google Scholar]
  17. Rosén S., Ek B., Rask L., Tunlid A. 1992; Purification and characterization of a surface lectin from the nematode-trapping fungus Arthrobotrys oligospora. Journal of General Microbiology 138:2663–2672
    [Google Scholar]
  18. Rosenzweig W. D., Ackroyd D. 1983; Binding characteristics of lectins involved in the trapping of nematodes by fungi. Applied and Environmental Microbiology 46:1093–1096
    [Google Scholar]
  19. Shepherd A. M. 1955; Formation of the infection bulb in Arthrobotrys oligospora Fresenius. Nature; London: 175475
    [Google Scholar]
  20. Smith K. C., Hanawalt P. C. 1969; Photochemistry of amino adds and proteins. In Molecular Photobiology. Inactivation and Recovery pp. 85–95 Smith K. C., Hanawalt P. C. Edited by New York & London: Academic Press;
    [Google Scholar]
  21. Sokal R. R., Rohlf F. J. 1981 Biometry. The principles and practice of statistics in biological research, 2nd edn. pp. 427–428 San Francisco: W. H. Freeman;
    [Google Scholar]
  22. Tunlid A., Janson S. 1991; Proteases and their involvement in the infection and immobilization of nematodes by the nematophagous fungus Arthrobotrys oligospora. Applied and Environmental Microbiology 57:2868–2872
    [Google Scholar]
  23. Veenhuis M., Nordbring-Hertz B., Harder W. 1985; An electron-microscopical analysis of capture and initial stages of penetration of nematodes by Arthrobotrys oligospora. Antonie van Leeuwenhoek 51:385–398
    [Google Scholar]
  24. Veenhuis M., Harder W., Nordbring-Hertz B. 1989; Occurrence and metabolic significance of microbodies in trophic hyphae of the nematophagous fungus Arthrobotrys oligospora. Antonie van Leeuwenhoek 56:241–249
    [Google Scholar]
  25. Wharton D. A., Murray D. S. 1990; Carbohydrate/lectin interactions between the nematophagous fungus Arthrobotrys oligospora, and the infective juveniles of Trichostrongylus colubriformis (Nematoda). Parasitology 101:101–106
    [Google Scholar]
  26. Whitaker-Dowling P., Youngner J. S. 1988; Alteration of vesicular stomatitis virus L and NS proteins by uv irradiation: implications for the mechanism of host cell shut-off. Virology 164:171–175
    [Google Scholar]
  27. Wimble D. B., Young T. W. K. 1984; Ultrastructure of the infection of nematodes by Dactylella lysipaga. Nova Hedwigia 40:9–29
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-11-2841
Loading
/content/journal/micro/10.1099/00221287-139-11-2841
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error