1887

Abstract

Cell walls were prepared from four strains belonging to group 2 alkaliphilic spp. Non-peptidoglycan components were extracted, with trichloroacetic acid, from the cell wall preparations and isolated by DEAE-cellulose column chromatography. All the components were acidic, and were composed of amino acids and sugars. Several components with different compositions were detected. The cell walls commonly contained teichuronopeptide, composed of polyglucuronic acid and a polypeptide of acidic amino acids.

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/content/journal/micro/10.1099/00221287-139-11-2739
1993-11-01
2021-07-27
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References

  1. Aono R. 1985; Isolation and partial characterization of structural components of the walls of alkalophilic Bacillus strain C-125. Journal of General Microbiology 131:105–111
    [Google Scholar]
  2. Aono R. 1987; Characterization of structural components of cell walls of alkalophilic strain of Bacillus sp. C-125. Biochemical Journal 245:467–472
    [Google Scholar]
  3. Aono R. 1989; Characterization of cell wall components of the alkalophilic Bacillus strain C-125: Identification of a polymer composed of polyglutamate and polyglucuronate. Journal of General Microbiology 135:265–271
    [Google Scholar]
  4. Aono R., Horikoshi K. 1983; Chemical composition of cell walls of alkalophilic strains of Bacillus. Journal of General Microbiology 129:1083–1087
    [Google Scholar]
  5. Aono R., Uramoto M. 1986; Presence of fucosamine in teichuronic acid of the alkalophilic Bacillus strain C-125. Biochemical Journal 233:291–294
    [Google Scholar]
  6. Aono R., Horikoshi K., Goto S. 1984; Composition of the peptidoglycan of alkalophilicBacillus spp. Journal of Bacteriology 157:688–689
    [Google Scholar]
  7. Beutler H. O., Michal G. 1974; Glutamate, determination with glutamate dehydrogenase, diaphorase and tetrazolium salts. In Methods of Enzymatic Analysis, 2nd edn. 4 pp. 1708–1713 Bergmeyer H. U. Edited by Weinheim: Verlag Chemie;
    [Google Scholar]
  8. Davidson E. A. 1966; Analysis of sugars found in mucopolysaccharides. Methods in Enzymology 8:52–60
    [Google Scholar]
  9. Dische Z. 1947; A specific color reaction for glucuronic acid. Journal of Biological Chemistry 171:725–730
    [Google Scholar]
  10. Edge A. S. B., Faltyner C. R., Hof L., Reichert L. E. Jr Weber P. 1981; Deglycosylation of glycoproteins by trifluoro-methanesulfonic acid. Analytical Biochemistry 118:131–137
    [Google Scholar]
  11. Grassl M., Supp M. 1985; d-Alanine. In Methods of Enzymatic Analysis, 3rd edn. 8 pp. 336–340 Bergmeyer H. U. Edited by Weinheim: VCH Verlagsgesellschaft;
    [Google Scholar]
  12. Ito M., Aono R., Horikoshi K. 1993; Identification of quinovosamine in cell wall components from alkaliphilic strain of Bacillus sp. Y-25 by 500 MHz NMR. Carbohydrate Research 242:173–180
    [Google Scholar]
  13. Möllering H. 1985; l-Aspartate and l-asparagine. In Methods of Enzymatic Analysis, 3rd edn. 8 pp. 350–357 Bergmeyer H. U. Edited by Weinheim: VCH Verlagsgesellschaft;
    [Google Scholar]
  14. Spies J. R. 1957; Colorimetric analysis for amino acids. Methods in Enzymology 3:467–477
    [Google Scholar]
  15. Spiro R. G. 1966; Analysis of sugars found in glycoprotein. Methods in Enzymology 8:3–26
    [Google Scholar]
  16. Stepka W. 1957; Identification of amino acids by paper chromatography. Methods in Enzymology 3:504–528
    [Google Scholar]
  17. Takasaki S., Mizouchi T., Kobata T. 1982; Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides. Methods in Enzymology 83:263–268
    [Google Scholar]
  18. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature: London; 166444–445
    [Google Scholar]
  19. Wheat R. W. 1966; Analysis of hexosamines in bacterial polysaccharides by chromatographic procedures. Methods in Enzymology 8:60–78
    [Google Scholar]
  20. Williamson D. H. 1985; l-Alanine: determination with alanine dehydrogenase. In Methods of Enzymatic Analysis, 3rd edn. 8 pp. 341–344 Bergmeyer H. U. Edited by Weinheim: VCH Verlagsgesellschaft;
    [Google Scholar]
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