1887

Abstract

A polysaccharide of molecular mass at least 300000 Da was isolated from walls of NCIB 7581. Three sugars, -lactyl-3-amino-3,6-dideoxyhexose, -acetylglucosamine and glucose were present in equimolar proportions, and accounted for 90% of the carbon of the polysaccharide. The remainder of the polymer was an unidentified acidic molecule, with a hydrophobic nature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-11-2731
1993-11-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/11/mic-139-11-2731.html?itemId=/content/journal/micro/10.1099/00221287-139-11-2731&mimeType=html&fmt=ahah

References

  1. Adam A., Petit J. F., Wietzerbin-Falszpan J., Sinay P., Thomas D. W., Lederer E. 1969; L’acide N-glycolyl-muramique, constituant des parois de Mycobacterium smegmatis: identification par spectrometrie de masse. FEBS Letters 4:87–92
    [Google Scholar]
  2. Ashwell G., Brown N. C., Volk W. A. 1965; A colorimetric procedure for the determination of N-acetylated-3-amino hexose. Archives of Biochemistry and Biophysics 112:648–652
    [Google Scholar]
  3. Banoub J. H., Shaw P. H. 1981; Isolation and characterization of 3-acetamido-3,6-dideoxyglucose from the core oligosaccharides obtained from the aquatic Gram-negative bacteria Aeromonas hydrophila and Vibrio anguillarum. Canadian Journal of Biochemistry 59:877–879
    [Google Scholar]
  4. Barker S. B. 1957; Preparation and colorimetric determination of lactic acid. Methods in Enzymology 3:241–246
    [Google Scholar]
  5. Bergmeyer H.-U., Bernt E. 1963; Determination with glucose oxidase and peroxidase. In Methods of Enzymatic Analysis, 1st edn. pp. 123–130 Bergmeyer H.-U. Edited by New York & London: Academic Press;
    [Google Scholar]
  6. Bishop A. H., Douglas C. W. I., Dorr P. K., White P. J. 1993; Components of the wall and capsule of Bacillus megaterium NCIB 7581. Journal of General Microbiology 139:2723–2730
    [Google Scholar]
  7. Burton R. M. 1957; The determination of glycerol and dihydroxy-acetone. Methods in Enzymology 3:246–249
    [Google Scholar]
  8. Conrad H. E., Bamburg J. R., Epley J. D., Kindt T. J. 1966; The structure of the Aerobacter aerogenes A3 (S1) polysaccharide. II. Sequence analysis and hydrolysis studies. Biochemistry (A.C.S.) 5:2808–2817
    [Google Scholar]
  9. Crumpton M. J., Davies D. A. L. 1958; The isolation of d-fucosamine from the specific polysaccharide of Chromobacterium violaceum (N.C.T.C. 7917). Biochemical Journal 70:729–736
    [Google Scholar]
  10. Davidson E. A. 1966; Analysis of sugars found in mucopolysaccharides. Modified carbazole procedure. Methods in Enzymology 8:55–56
    [Google Scholar]
  11. Devor A. W. 1950; Carbohydrate tests using sulfonated α-naphthol. Journal of the American Chemical Society 72:2008–2012
    [Google Scholar]
  12. Dische Z., Shettles L. B. 1948; A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. Journal of Biological Chemistry 175:595–603
    [Google Scholar]
  13. Dixon J. S., Lipkin D. 1954; Spectrophotometric determination of vicinal glycols. Application to the determination of ribofuranosides. Analytical Chemistry 26:1092–1093
    [Google Scholar]
  14. Dutton G. G. S., Lim A. V. S. 1985; Structure of the capsular polysaccharide of Klebsiella serotype K79. Carbohydrate Research 144:263–276
    [Google Scholar]
  15. Dutton G. G. S., Parolis H., Parolis L. A. S. 1985; A structural investigation of the capsular polysaccharide of Klebsiella K14. Carbohydrate Research 140:263–275
    [Google Scholar]
  16. Ellwood D. C., Tempest D. W. 1972; Effects of environment on bacterial wall content and composition. Advances in Microbial Physiology 7:83–117
    [Google Scholar]
  17. Gardell S. 1953; Separation on Dowex 50 ion exchange resin of glucosamine and galactosamine and their quantitative determination. Acta Chemica Scandinavica 7:207
    [Google Scholar]
  18. Gawehn K., Bergmeyer H. U. 1974; d-(—)-Lactate. In Methods of Enzymatic Analysis, 2nd English edn. 3 pp. 1492–1495 Bergmeyer H. U. New York & London: Academic Press;
    [Google Scholar]
  19. Goldstein I. J., So L. L. 1965; Protein-carbohydrate interaction. III. Agar-gel diffusion studies on the interaction of concanavalin A, a lectin isolated from jack bean, with polysaccharides. Archives of Biochemistry and Biophysics 111:407–414
    [Google Scholar]
  20. Gordon H. T., Thornburg W., Werum L. N. 1956; Rapid paper chromatography of carbohydrates and related compounds. Analytical Chemistry 28:849–855
    [Google Scholar]
  21. Gutmann I., Wahlfeld A. W. 1974; l-(+)-Lactate. Determination with lactate dehydrogenase and NAD. In Methods of Enzymatic Analysis 2nd English edn 3 pp. 1464–1468 Bergmeyer H. U. New York & London: Academic Press;
    [Google Scholar]
  22. Hash J. H., Rothlauf M. W. 1967; The N,O-diacetylmuramidase of Chalaropsis species. Journal of Biological Chemistry 102:379–388
    [Google Scholar]
  23. Hayashi H., Araki Y., Ito E. 1973; Occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme. Journal of Bacteriology 113:592–598
    [Google Scholar]
  24. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5:209–344
    [Google Scholar]
  25. Hickman J., Ashwell G. 1966; Isolation of a bacterial lipopolysaccharide from Xanthomonas campestris containing 3-acetamido-3,6-dideoxy d-galactose and d-rhamnose. Journal of Biological Chemistry 241:1424–1428
    [Google Scholar]
  26. Howe J. R. 1960; Paper chromatography and structural relationships of organic acids. Journal of Chromatography 3:389–405
    [Google Scholar]
  27. Ivatt R. J., Gilvarg C. 1978; Molecular structure of the teichuronic acid of Bacillus megaterium.. Biochemistry(A.C.S.) 17:3997–4003
    [Google Scholar]
  28. Jann B., Jann K., Muller-Seitz E. 1967; A 3-amino-3,6-dideoxyhexose from the lipopolysaccharide of Escherichia coli O71. Nature: London; 215170–171
    [Google Scholar]
  29. Joseleau J.-P. 1985; Structural investigation of the capsular polysaccharide of Klebsiella serotype K49. Carbohydrate Research 142:85–92
    [Google Scholar]
  30. Kaya S., Araki Y., Ito E. 1985; Characterization of a novel linkage unit between ribitol teichoic acid and peptidoglycan in Listeria monocytogenes cell walls. European Journal of Biochemistry 146:517–522
    [Google Scholar]
  31. Kent P. W., Whitehouse M. W. 1955 Biochemistry of the Amino Sugars, 1st edn. p. 270 London: Butterworths Scientific Publications;
    [Google Scholar]
  32. Khym J. X., Zill L. P. 1951; The separation of sugars by ion exchange. Journal of the American Chemical Society 74:2090–2094
    [Google Scholar]
  33. Knirel Y. A., Vinogradov E. V., Shashkov A. S., Dimitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1985; Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of lipopolysaccharides of P.aeruginosa O4 (Lanyi) and related serotype O6 (Habs) and immunotype 1 (Fisher). European Journal of Biochemistry 150:541–550
    [Google Scholar]
  34. Knirel Y. A., Vinogradov E. V., Shashkov A. S., Dimitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. 1986; Somatic antigens of Pseudomonas aeruginosa. The structure of O-specific polysaccharide chains of P. aeruginosa O10 (Lanyi) lipopolysaccharides. European Journal of Biochemistry 157:129–138
    [Google Scholar]
  35. Kojima N., Araki Y., Ito E. 1985; Structural studies on the acidic polysaccharides of Bacillus cereus AHU 1356 cell walls. European Journal of Biochemistry 148:479–484
    [Google Scholar]
  36. Levvy G. A., Mcallan A. 1959; The N-acetylation and estimation of hexosamines. Biochemical Journal 73:127–132
    [Google Scholar]
  37. Luderitz O., Ruschmann E., Westphal O., Raff R., Wheat W. 1967; Occurrence of 3-amino-3,6-dideoxyhexose in Salmonella and related bacteria. Journal of Bacteriology 93:1681–1687
    [Google Scholar]
  38. Macfadyen D. A. 1945; Estimation of formaldehyde in biological mixtures. Journal of Biological Chemistry 158:107–135
    [Google Scholar]
  39. Raff R. A., Wheat R. W. 1967; Characterization of 3-amino-3,6-dideoxy-d-glucose from a bacterial lipopolysaccharide. Journal of Biological Chemistry 242:4610–4613
    [Google Scholar]
  40. Renkonen O., Schindler O. 1956; Papierchromatographische Trennung der naturlichen 2-Deoxyhemamethylosen und deren 3-Methylather. Helvetica Chimica Acta 39:1490–1493
    [Google Scholar]
  41. Rochas C, Lahaye M., Yaphe W., Viet M. J. P. 1986; 13C.N.M.R.-spectroscopic investigation of agarose oligomers. Carbohydrate Research 148:199–207
    [Google Scholar]
  42. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochemical Journal 61:586–589
    [Google Scholar]
  43. Sasaki Y., Araki Y., Ito E. 1983; Structure of teichoicacid-glycopeptide complexes from cell walls of Bacillus cereus AHU 1030. European Journal of Biochemistry 132:207–213
    [Google Scholar]
  44. Sykora V., Prochazka Z. 1953; Identifikace 2,4-dinitrofenyl-hydrazonukarbonylovych sloucenin papirovou chromatografii. Chemicke Listy 47:1674–1675
    [Google Scholar]
  45. Tipper D. J. 1968; Alkali-catalysed elimination of d-lactic acid from muramic add and its derivatives and the determination of muramic acid. Biochemistry (A.C.S.) 7:1441–1449
    [Google Scholar]
  46. Warth A. D., Strominger J. L. 1969; Structure of the peptidoglycan of bacterial spores : occurrence of the lactam of muramic add. Proceedings of the National Academy of Sciences of the United States of America 64:528–535
    [Google Scholar]
  47. Westmacott D., Perkins H. R. 1979; Effects of lysozyme on Bacillus cereus 569: rupture of chains of bacteria and enhancement of sensitivity to autolysins. Journal of General Microbiology 115:1–11
    [Google Scholar]
  48. White P. J., Gilvarg C. 1977; A teichuronic acid containing rhamnose from cell walls of Bacillus megaterium.. Biochemistry (A.C.S.) 16:2428–2435
    [Google Scholar]
  49. Whitehouse M. W., Kent P. W., Pasternak C. A. 1954; Acyclic derivatives of amino-sugars. Journal of the Chemical Society2315–2317
    [Google Scholar]
  50. Work E. 1957; Reaction of ninhydrin in acid solution with straight chain aminoadds containing two amino groups and its application to the estimation of α,ɛ-diaminopimelic acid. Biochemical Journal 67:416–423
    [Google Scholar]
/content/journal/micro/10.1099/00221287-139-11-2731
Loading
/content/journal/micro/10.1099/00221287-139-11-2731
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error