Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific Enzyme II (lacE) gene of the PTS and the phospho-β-galactosidase (lacG) gene
The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-β-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. The S. mutans lacE and lacG genes are located in the same operon as the tagatose genes. S. mutans metabolizes lactose via the tagatose phosphate pathway. The deduced LacE and LacG proteins of S. mutans display high homology with the corresponding proteins from Lactococcus lactis, Staphylococcus aureus and Lactobacillus casei.
AlpertC.-A.,
ChassyB. M.1990; Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific Enzyme II of the phosphotransferase system of Lactobacillus casei. Journal of Biological Chemistry 265:22561–22568
BissetD.,
AndersonR. L.1973; Lactose and d-galactose metabolism in Staphylococcus aureus: pathway of d-galactose 6-phosphate degradation. Biochemical and Biophysical Research Communications 52:641–647
BissetD.,
AndersonR. L.1974; Genetic evidence for the physiological significance of the d-tagatose 6-phosphate pathway of lactose and d-galactose degradation in Staphylococcus aureus. Journal of Bacteriology 119:698–704
BreidtF.JrStewartG. C.1987; Nucleotide and deduced amino acid sequences of the Staphylococcus aureus phospho-beta-galactosidase gene. Applied and Environmental Microbiology 53:969–973
BreidtF.JrHengstenbergW.,
FinkeldeiU.,
StewartG. C.1987; Identification of the genes for the lactose-specific components of the phosphotransferase system in the lac operon of Staphylococcus aureus. Journal of Biological Chemistry 262:16444–16449
De VosW. M.,
GassonM. J.1989; Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis. Journal of General Microbiology 135:1833–1846
De VosW. M.,
BoerrigterI.,
Van RooyenR. J.,
ReidheB.,
HengstenbergW.1990; Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis. Journal of Biological Chemistry 265:22554–22560
FrostellG.,
KeyesP. H.,
LarsonR. H.1967; Effect of various sugars and sugar substitutes on dental caries in hamsters and rats. Journal of Nutrition 93:65–76
GuggenheimB.,
KonigK. G.,
HerzogE.,
MuhlemannH. R.1966; The cariogenicity of different dietary carbohydrates tested on rats in relative gnotobiosis with a streptococcus producing extracellular polysaccharide. Helvetica Odontologica Acta 10:101–113
HamiltonI. R.,
LebtagH.1979; Lactose metabolism by Streptococcus mutans: evidence for induction of the tagatose 6-phosphate pathway. Journal of Bacteriology 140:1102–1104
ManoilC.,
BeckwithJ.1985; TnphoA: a transposon probe for protein export signals. Proceedings of the National Academy of Sciences of the United States of America 82:8129–8133
MoranC. P.JrLangN.,
LegriceS.,
LeeG.,
StephensM.,
SonensheinA. L.,
PeroJ.,
LosickR.1982; Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Molecular and General Genetics 186:339–346
MurchisonH.,
LarrimoreS.,
CurtissR.III1981; Isolation and characterization of Streptococcus mutans mutants defective in adherence and aggregation. Infection and Immunity 34:1044–1055
MurchisonH. H.,
BarrettJ. F.,
CardineauG. A.,
CurtissR.III1986; Transformation of Streptococcus mutans with chromosomal and shuttle plasmid (pYA629 DNAs. Infection and Immunity 54:273–282
PearsonW. R.,
LipmanD. J.1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences of the United States of America 85:2444–2448
PorterE. V.,
ChassyB. M.1988; Nucleotide sequence of the beta-d-phosphogalactoside galactohydrolase gene of Lactobacillus casei: comparison to analogous pbg genes of other Gram-positive organisms. Gene 62:263–276
RoseyE. L.,
StewartG. C.1992; Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific PTS components of the lactose operon of Streptococcus mutans. Journal of Bacteriology 174:6159–6170
RoseyE. L.,
OskouianB.,
StewartG. C.1991; Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway. Journal of Bacteriology 173:5992–5998
SmorawinskaM.,
HsuJ. C.,
HansenJ. B.,
Jagusztyn-KrynickaE. K.,
AbikoY.,
CurtissR.III1983; Clustered genes for galactose metabolism from Streptococcus mutans cloned in Escherichia coli. Journal of Bacteriology 153:1095–1097
TaborS.,
RichardsonC. C.1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 84:4767–4771
Van RooijenR. J.,
Van SchalkwijkS.,
De VosW. M.1991; Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis. Journal of Biological Chemistry 266:7176–7181
Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific Enzyme II (lacE) gene of the PTS and the phospho-β-galactosidase (lacG) gene