Immunological specificity of oral species Free

Abstract

Antigens of species including subsp. and subsp. , which have been isolated frequently from periodontal pockets and associated with periodontal diseases, were extracted by ultrasonication from whole bacterial cells. Antigens were also prepared from and , which have been found in intestinal tracts and infected abscesses in human oral cavities. The antigens of the oral species were compared with antigens from , the type species of the genus , by using SDS-PAGE and Western immunoblot assays. SDS-PAGE gels stained with Coomassie brilliant blue indicated that no major peptide bands were common among the species examined. The protein profile patterns were distinctly different from each other. Western immunoblotting reactions with rabbit antisera showed that the species could be clearly distinguished serologically, and that the species-specific antigens were peptide components of ultrasonic extracts from the whole bacterial cells. The present study demonstrates that these species show great heterogeneity in their peptide components and immunological reactions, which may be useful for identification of the species from human oral specimens.

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/content/journal/micro/10.1099/00221287-139-11-2635
1993-11-01
2024-03-29
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References

  1. Ando N., Hoshino E. 1990; Predominant obligate anaerobes invading the deep layers of root canal dentine. International Endodontic Journal 23:20–27
    [Google Scholar]
  2. Burnette W. N. 1981; Western blotting electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodilied nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195
    [Google Scholar]
  3. Edwardsson S. 1974; Bacteriological studies on deep areas of carious dentine. Odontolgisk Revy 25: suppl. 32 1–143
    [Google Scholar]
  4. Faye L. M. J., Chrispeels M. L. 1985; Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A peroxidase and treatment of the blots with glycosidases. Analytical Biochemistry 149:218–224
    [Google Scholar]
  5. Gunsolley J. C., Tew J. G., Gooss C., Marshall D. R., Burmeister J. A., Schenkein H. A. 1990; Serum antibodies to periodontal bacteria. Journal of Periodontology 61:412–419
    [Google Scholar]
  6. Han N., Xiao X., Zhang L., Ri X., Zhang J., Tong Y., Yang M., Xiao Z. 1991; Bacteriological study of juvenile periodontitis in China. Journal of Periodontal Research 26:409–414
    [Google Scholar]
  7. Hill G. B., Ayers O. M., Kohan A. P. 1987; Characteristics and sites of infection of Eubacterium nodatum, Eubacterium timidum, Eubacterium brachy, and other asaccharolytic eubacteria. Journal of Clinical Microbiology 25:1540–1545
    [Google Scholar]
  8. Hodge J. E., Hofreiter B. T. 1962; Phenol-sulfuric acid colorimetric method. Methods of Carbohydrate Chemistry 1:388–394
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Media. In Anaerobe Laboratory Manual, 4th edn. pp. 141–147 Holdeman L. V., Cato E. P., Moore W. E. C. Edited by Blacksburg: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Holdeman L. V., Cato E. P., Burmeister J. A., Moore W. E. C. 1980; Descriptions of Eubacterium timidum sp. nov., Eubacterium brachy sp. nov., and Eubacterium nodatum sp. nov isolated from human periodontitis. International Journal of Systematic Bacteriology 30:163–169
    [Google Scholar]
  11. Hoshino E. 1985; Predominant obligate anaerobes in human carious dentine. Journal of Dental Research 64:1195–1198
    [Google Scholar]
  12. Hoshino E., Ando N., Sato M., Kota K. 1992a; Bacterial invasion of non-exposed dental pulp. International Endodontic Journal 25:2–5
    [Google Scholar]
  13. Hoshino E., Sato N., Nakazawa F., Uematsu H., Sato M., Ikeda T., Kurihara N., Sato T., Sato M. M. 1992b; Oral Eubacterium. Niigata Dental Journal 22:1–14
    [Google Scholar]
  14. Kondo W., Nakazawa F., Sato M., Ito T. 1983; Structural studies of the antigenic polysaccharide of Eubacterium saburreum, strain T27. Carbohydrate Research 117:125–131
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  16. Margaret B. S., Krywolap G. N. 1986; Eubacterium yurii subsp.yurii sp. nov. and Eubacterium yurii subsp.margaretiae subsp. nov.: test tube brush bacteria from subgingival dental plaque. International Journal of Systematic Bacteriology 36:145–149
    [Google Scholar]
  17. Margaret B. S., Krywolap G. N. 1988; Eubacterium yurii subsp.schtitka subsp. nov.: test tube brush bacteria from subgingival dental plaque. International Journal of Systematic Bacteriology 38:207–208
    [Google Scholar]
  18. Martin S. A., Falkler W. A. Jr Vincent J. W., Mackler B. F., Suzuki J. B. 1986; A comparison of the reactivity of Eubacterium species with localized and serum immunoglobulins from rapidly progressive and adult periodontitis patients. Journal of Periodontology 59:32–39
    [Google Scholar]
  19. Mashimo P. A., Murayama Y., Reynolds H., Mouton C., Ellison S. A., Genco R. J. 1981; Eubacterium saburreum and Veillonella parvula. Journal of Periodontology 52:374–379
    [Google Scholar]
  20. Moore W. E. C., Moore L. V. H. 1986; Genus Eubacterium. In Bergey’s Manual of Systematic Bacteriology pp. 1353–1372 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Edited Baltimore: Williams & Wilkins;
    [Google Scholar]
  21. Moore W. E. C., Holdeman L. V., Smibert R. M., Hash D. E., Burmeister J. A., Ranney R. R. 1982a; Bacteriology of severe periodontitis in young adult humans. Infection and Immunity 38:1137–1148
    [Google Scholar]
  22. Moore W. E. C., Ranney R. R., Holdeman L. V. 1982b; Subgingival microflora in periodontal disease: culture studies. In Host Parasite Interaction in Periodontal Disease pp. 13–26 Genco R. J., Mergenhagen S. E. Edited by Washington DC: American Society for Microbiology;
    [Google Scholar]
  23. Moore W. E. C., Holdeman L. V., Cato E. P., Smibert R. M., Burmeister J. A., Ranney R. R. 1983; Bacteriology of moderate (chronic) periodontitis in mature adult humans. Infection and Immunity 42:510–515
    [Google Scholar]
  24. Moore W. E. C., Holdeman L. V., Cato E. P., Smibert R. M., Burmeister J. A., Palcanis K. G., Ranney R. R. 1985; Comparative bacteriology of juvenile periodontitis. Infection and Immunity 48:507–519
    [Google Scholar]
  25. Nakazawa F. 1985; Structural studies of the antigenic polysaccharide of Eubacterium saburreum, strain T17. Carbohydrate Research 143:185–190
    [Google Scholar]
  26. Nakazawa F., Hoshino E. 1992; Immunochemical and structural characterization of the antigenic polysaccharide from Eubacterium saburreum T18. Oral Microbiology and Immunology 7:182–186
    [Google Scholar]
  27. Nakazawa F., Ito T., Sato N., Sato M., Hoshino E. 1987; Chemical composition and immunological characterization of polysaccharide antigen from Eubacterium saburreum T18. Infection and Immunity 55:871–876
    [Google Scholar]
  28. Sandholm L. 1985; Bacteriology of juvenile periodontitis. A review. Proceedings of the Finnish Dental Society 81:63–76
    [Google Scholar]
  29. Sandholm L. 1987; Serum and salivary antibodies in various periodontal conditions. Proceedings of the Finnish Dental Society 83:151–155
    [Google Scholar]
  30. Sandholm L., Tolo K. 1986; Serum antibody levels to 4 periodontal pathogens remain unaltered after mechanical therapy of juvenile periodontitis. Journal of Clinical Periodontology 13:646–650
    [Google Scholar]
  31. Skaug N., Hofstad T. 1983; Eubacterium saburreum L13 antigen containing a ketohexose as a main sugar constitutent. Infection and Immunity 39:179–183
    [Google Scholar]
  32. Tanner A. C. R., Socransky S. S., Goodson J. M. 1984; Microbiota of periodontal pockets losing crestal alveolar bone. Journal of Periodontal Research 19:279–291
    [Google Scholar]
  33. Tew J. G., Mareshall D. R., Moore W. E. C., Best A. M., Palcanis K. G., Ranney R. R. 1985; Serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. Infection and Immunity 48:303–311
    [Google Scholar]
  34. Theilade E., Fejerskov O., Karring T., Theilade J. 1982; Predominant cultivable microflora of human dental fissure plaque. Infection and Immunity 36:977–982
    [Google Scholar]
  35. Tolo K., Jorkjend L. 1990; Serum antibodies and loss of periodontal bone in patients with rheumatoid arthritis. Journal of Clinical Periodontology 17:288–291
    [Google Scholar]
  36. Tolo K., Schenck K. 1985; Activity Of serum immunoglobulins G, A and M to six anaerobic, oral bacteria in diagnosis of periodontitis. Journal of Periodontal Research 20:113–121
    [Google Scholar]
  37. Uematsu H., Hoshino E. 1992; Predominant obligate anaerobes in human periodontal pockets. Journal of Periodontal Research 27:15–19
    [Google Scholar]
  38. Vincent J. K., Falkler W. A. Jr Heath J. R. III 1984; Eubacterium brachy: reactivity in in vitro bone resorptive bioassay. Journal of Periodontology 55:93–97
    [Google Scholar]
  39. Vincent J. K., Falkler W. A. Jr Dalessandro N. F., Miller R. A., Heath J. R. III 1985; Reaction of human sera with Eubacterium brachy : isolation and characterization of an extracellular antigen. Infection and Immunity 47:592–597
    [Google Scholar]
  40. Wade W. G., Gray A. R., Absi E., Barket G. R. 1991; Predominant cultivable flora in pericoronitis. Oral Microbiology and Immunology 6:310–312
    [Google Scholar]
  41. Wade W. G., Moran J., Morgan J. R., Newcombe R., Addy M. 1992; The effects of antibacterial acrylic strips on the subgingival microflora in chronic periodontitis. Journal of Clinical Periodontology 19:127–134
    [Google Scholar]
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