1887

Abstract

Out of 22 phages investigated, nine were found to be suitable for phage typing based on their different lytic spectra on 398 strains of spp. isolated from milk powder and other foods. These phages were compared on the basis of morphology, protein composition, restriction endonuclease patterns and DNA-DNA hybridization. Two phages (WS-EP19, WS-EP13) belonged to the Podoviridae family (morphotype C1), and three (WS-EP20, WS-EP26, WS-EP28) were classified as Siphoviridae (morphotype B1). The other four phages were Myoviridae of the morphological groups A1 (WS-EP57) and A2 (WS-EP32, WS-EP94, WS-EP96). SDS-PAGE revealed individual protein profiles for each phage, which corresponded to different restriction enzyme fragment patterns. DNA-DNA hybridization demonstrated the close relationship of phages WS-EP20 and WS-EP26, and of WS-EP94 and WS-EP96. In general, a good correlation was found between groupings obtained with the various methods. The nine phages could be attributed to existing enterobacterial phage species although some differences to the described type phages were observed.

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1993-11-01
2024-03-29
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References

  1. Ackermann H.-W., Dubow M. S. 1987; Enterobacterial phages. In Viruses of Procaryotes I General Properties of Bacterio-phages pp. 143–150 II Natural Groups of Bacteriophages pp. 85–100 Boca Raton: CRC Press;
    [Google Scholar]
  2. Ackermann H.-W., Eisenstark A. 1974; The present state of phage taxonomy. Intervirology 3:201–219
    [Google Scholar]
  3. Adams M. H. 1959; Methods of study of bacterial viruses. In Bacteriophages pp. 443–457 New York: Interscience publishers;
    [Google Scholar]
  4. Arendt E. K., Lonvaud A., Hammes W. P. 1991; Lysogeny in Leuconostoc oenos. Journal of General Microbiology 137:2135–2139
    [Google Scholar]
  5. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  6. Braun V. Jr Hertwig S., Neve H., Geis A., Teuber M. 1989; Taxonomic differentiation of bacteriophages of Lactococcus lactis by electron microscopy, DNA-DNA hybridization, and protein profiles. Journal of General Microbiology 135:2551–2560
    [Google Scholar]
  7. Coveney J. A., Fitzgerald G. F., Daly C. 1987; Detailed characterization and comparison of four lactic streptococcal bacteriophages based on morphology, restriction mapping, DNA homology, and structural protein analysis. Applied and Environmental Microbiology 53:1439–1447
    [Google Scholar]
  8. Frazier W. C., Westhoff D. C. 1978 Food Microbiology, 3rd edn.. New York: McGraw-Hill;
    [Google Scholar]
  9. Grimont F., Grimont P. A. D. 1981; DNA relatedness among bacteriophages of the morphological group C3. Current Microbiology 6:65–69
    [Google Scholar]
  10. Grimont F., Grimont P. A. D., Du Pasquier P. 1978; Morphological study of five bacteriophages of yellow-pigmented enterobacteria. Current Microbiology 1:37–40
    [Google Scholar]
  11. Keogh B. P. 1971; Reviews of the progress of dairy science. Journal of Dairy Research 38:91–111
    [Google Scholar]
  12. Loessner M. J. 1991; Improved procedure for bacteriophage typing of Listeria strains and evaluation of new phages. Applied and Environmental Microbiology 57:882–884
    [Google Scholar]
  13. Loessner M. J., Busse M. 1990; Bacteriophage typing of Listeria species. Applied and Environmental Microbiology 56:1912–1918
    [Google Scholar]
  14. Luftig R. 1967; An accurate measurement of the catalase crystal period and its use as an internal marker for electron microscopy. Journal of Ultrastructure Research 20:91–102
    [Google Scholar]
  15. Maftahi M. 1992 Etude moléculaire comparative de 18 bactériophages appartenant au morphospecies T-pair. Thesis (DEA) Universités Paris V and XI:
    [Google Scholar]
  16. Mata M., Trautwetter A., Luthaud G., Ritzenthaler P. 1986; Thirteen virulent and temperate bacteriophages of Lactobacillus bulgaricus and Lactobacillus lactis belong to a single DNA homology group. Applied and Environmental Microbiology 52:812–818
    [Google Scholar]
  17. Popovici M., Cosman M., Filipescu S., Voicescu V., Olinescu E. 1976; Taxonomic relationships Klebsiella-Enterobacter investigated by means of phage-bacteria systems. Archives Roumaines de Pathologie Experimental et de Microbiologie 35:187–194
    [Google Scholar]
  18. Prevot F., Relano P., Mata M., Ritzenthaler P. 1989; Close relationship of virulent bacteriophages of Streptococcus salivarius subsp.thermophilus at both the protein and the DNA level. Journal of General Microbiology 135:3337–3344
    [Google Scholar]
  19. Sakazaki R., Namioka S. 1960; Serological studies on the Cloaca (Aerobacter) groups of enteric bacteria. Japanese Journal of Medical Science and Biology 13:1–12
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  21. Schmidt-Lorenz W., Spillmann H. 1988; Kritische Überlegungen zum Aussagewert von E. coli, Coliformen und Enterobacteriaceen in Lebensmitteln. Archiv für Lebensmittelhygiene 39:3–15
    [Google Scholar]
  22. Stewart P. R., Waldron H. G., Lee J. S., Matthews P. R. 1985; Molecular relationships among serogroup B bacteriophages of Staphylococcus aureus. Journal of Virology 55:111–116
    [Google Scholar]
  23. Warren R. A. J. 1980; Modified bases in bacteriophage DNAs. Annual Reviews in Microbiology 34:137–158
    [Google Scholar]
  24. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  25. Zink R., Loessner M. J. 1992; Classification of virulent and temperate bacteriophages of Listeria spp. on the basis of morphology and protein analysis. Applied and Environmental Microbiology 58:296–302
    [Google Scholar]
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