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Volume 139, Issue 11

Evidence for of non-human origin Free

Abstract

This paper describes the characterization and taxonomic status of N16, a chlamydial isolate from the respiratory tract of a horse. N16 contains plasmid DNA, has normal elementary body morphology and its inclusions do not stain with iodine. Its major outer-membrane protein (MOMP) gene was completely sequenced and compared with the MOMP genes of and This analysis revealed that N16 is closely related to the TWAR strain of (94·5% and 94·4% DNA homology with TWAR isolates IOL-207 and AR-39 respectively). By comparison, N16 shows between 72·1% and 73·7% DNA homology with strains, 70·9% and 71·1% homology with strains LW613 and 1710S and 69.2% homology with serotype E. The MOMP gene of N16 shares 93·8% DNA homology with the MOMP gene of a chlamydial isolate KC from the conjunctiva of a koala. Monoclonal antibodies raised to IOL-207 and shown to be -specific confirmed that N16 was more closely related to than to Thus DNA homology and monoclonal antibody data both suggest that horse chlamydiae, as exemplified by N16, form a new second strain of This species is probably more widespread and diverse than the current literature would suggest.

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  • Published Online:
© Society for General Microbiology 1993
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1993-11-01
2024-04-16
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Evidence for Chlamydia pneumoniae of non-human origin
Microbiology 139, 2621 (1993); https://doi.org/10.1099/00221287-139-11-2621
/content/journal/micro/10.1099/00221287-139-11-2621
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