The 16S rDNA sequences from 15 Leptospiraceae were determined by automated PCR-directed cycle sequencing. Nucleotide comparisons, including those from published sequences for Moulton and spp., were used to construct phylogenetic trees. and were related to each other but were distinct from the comprising parva incertae sedis (Turneria parva H), and spp. The pathogenic and the saprophytic leptospires were distinct and separated from each other.occupied an intermediate position between the two forms. The pathogens formed three groups. Group I was represented by stricto and , Group II by and , and Group III comprised and . The saprophytic species,and biflexa sensu stricto shared about 99% sequence similarity. The freshwater isolates were distinct from the marine isolate biflexa sensu lato ancona Ancona Porto.


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