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Volume 139, Issue 11

The use of 16S rDNA sequence analysis to investigate the phylogeny of and related spirochaetes Free

Abstract

The 16S rDNA sequences from 15 Leptospiraceae were determined by automated PCR-directed cycle sequencing. Nucleotide comparisons, including those from published sequences for Moulton and spp., were used to construct phylogenetic trees. and were related to each other but were distinct from the comprising parva incertae sedis (Turneria parva H), and spp. The pathogenic and the saprophytic leptospires were distinct and separated from each other. occupied an intermediate position between the two forms. The pathogens formed three groups. Group I was represented by stricto and , Group II by and , and Group III comprised and . The saprophytic species,and biflexa sensu stricto shared about 99% sequence similarity. The freshwater isolates were distinct from the marine isolate Porto.

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© Society for General Microbiology 1993
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1993-11-01
2024-04-23
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The use of 16S rDNA sequence analysis to investigate the phylogeny of Leptospiraceae and related spirochaetes
Microbiology 139, 2585 (1993); https://doi.org/10.1099/00221287-139-11-2585
/content/journal/micro/10.1099/00221287-139-11-2585
/content/journal/micro/10.1099/00221287-139-11-2585
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