1887

Abstract

SUMMARY: The possible functions of two recently described flagellar genes, and , have been examined. Introduction of gene , encoding the bacterial flagellin protein, into a number of flagellin-deficient and strains failed to complement the mutations in these strains, and the FliC flagellin was accumulated in the bacterial cytoplasm. Complementation with and , which map downstream of , restored motility to some of the mutants which became flagellated. After inactivation of either or , such complementation no longer occurred and the flagellin protein accumulated in the cytoplasm, which suggested that both genes are required for the secretion of flagellin and expression of motility. Expression of these genes from high copy number plasmids resulted in the synthesis of exceptionally long flagella and in detection of the FliV protein on polyacrylamide gels.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-10-2415
1993-10-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/10/mic-139-10-2415.html?itemId=/content/journal/micro/10.1099/00221287-139-10-2415&mimeType=html&fmt=ahah

References

  1. Bullas L.R., Ryu J.-I. 1983; Salmonella typhimurium LT2 strains which are r m+ for all three chromosomally located systems of DNA restriction and modification.. Journal of Bacteriology 256:471–474
    [Google Scholar]
  2. Depamphilis M.L., Adler J. 1971; Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis flagella.. Journal of Bacteriology 105:369–375
    [Google Scholar]
  3. Doll L., Frankel G. 1993; Cloning and DNA sequencing of two new fli genes, the products of which are essential for Salmonella flagellar biosynthesis.. Gene 126:119–121
    [Google Scholar]
  4. Frankel G., Newton S.M.C., Schoolnik G.K., Stocker B.A.D. 1989; Intragenic recombination in a flagellin gene: characterization of the Hl-j gene of Salmonella typhi. . EMBO Journal 10:3149–3152
    [Google Scholar]
  5. Homma M., Fujita J., Yamaguchi S., Iino T. 1987; Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion.. Journal of Bacteriology 169:291–296
    [Google Scholar]
  6. Homma M., Kutsukake K., Hasebe M., Iino T., Macnab R.M. 1990; FlgB, FlgC, FlgF and FlgG. A family of structurally related proteins in the flagellar basal body of Salmonella typhimurium. . Journal of Molecular Biology 211:465–477
    [Google Scholar]
  7. Iino T. 1969; Genetics and chemistry of bacterial flagella.. Bacteriological Reviews 33:454–475
    [Google Scholar]
  8. Iino T. 1974; Assembly of Salmonella flagellin in vitro and in vivo. . Journal of Supramolecular Structure 2:372–384
    [Google Scholar]
  9. Jones C.J., Macnab R.M. 1990; Flagellar assembly in Salmonella typhimurium: analysis of temperature-sensitive mutants.. Journal of Bacteriology 172:1327–1339
    [Google Scholar]
  10. Komeda Y., Suzuki H., Ishidsu J., Iino T. 1975; The role of cAMP in flagellation of Salmonella typhimurium. . Molecular and General Genetics 142:289–298
    [Google Scholar]
  11. Kuwajima G., Kawagishi I., Homma M., Asaka J.-I., Kondo E., Macnab R.M. 1989; Export of an N-terminal fragment of Escherichia coli flagellin by a flagellum-specific pathway.. Proceedings of the National Academy of Sciences of the United States of America 864953–4957
    [Google Scholar]
  12. Kutsukake K., Ohya Y., Iino T. 1990; Transcriptional analysis of the flagellar regulon of Salmonella typhimurium. . Journal of Bacteriology 172:741–747
    [Google Scholar]
  13. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  14. Macnab R.M. 1987; Flagella.. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 1 pp. 70–83 Edited by Neidhardt F. C, Ingraham J., Low K. B, Magasanik B., Schaechter M., Umbarger H. E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Macnab R.M., Parkinson J.S. 1991; Genetic analysis of the bacterial flagellum.. Trends in Genetics 7:196–200
    [Google Scholar]
  16. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mullis K.B., Faloona F.A. 1987; Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction.. Methods in Enzymology 155:335–350
    [Google Scholar]
  18. Namba K., Yamashita I., Vonderviszt F. 1989; Structure of core and central channel of bacterial flagella.. Nature, London 342:648–654
    [Google Scholar]
  19. Newton S.M.C., Jacob C.O., Stocker B.A.D. 1989; Immune response to cholera toxin epitope inserted in Salmonella flagellin.. Science 244:70–72
    [Google Scholar]
  20. Nishimura A., Hirota Y. 1989; A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli. . Molecular and General Genetics 216:340–346
    [Google Scholar]
  21. Ohnishi K., Kutsukake K., Suzuki H., Iino T. 1990; Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium. . Molecular and General Genetics 221:139–147
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  23. Silverman M., Simon M. 1974; Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression.. Journal of Bacteriology 120:1196–1203
    [Google Scholar]
  24. Trachtenberg S., Derosier D.J. 1987; Three-dimensional structure of the frozen-hydrate flagellar filament.. Journal of Molecular Biology 195:581–601
    [Google Scholar]
  25. Vogler A.P., Homma M., Irikura V.M., Macnab R.M. 1991; Salmonella typhimurium mutants defective in flagellar filament regrowth and similarity of Flil to F0Fj, vacuolar, and archaebacterial ATPase subunits.. Journal of Bacteriology 173:3564–3572
    [Google Scholar]
  26. Wei L.-N., Joys T.M. 1985; Covalent structure of three phase-1 flagellar filament proteins of Salmonella. . Journal of Molecular Biology 186:791–803
    [Google Scholar]
/content/journal/micro/10.1099/00221287-139-10-2415
Loading
/content/journal/micro/10.1099/00221287-139-10-2415
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error