1887

Abstract

SUMMARY: The possible functions of two recently described flagellar genes, and , have been examined. Introduction of gene , encoding the bacterial flagellin protein, into a number of flagellin-deficient and strains failed to complement the mutations in these strains, and the FliC flagellin was accumulated in the bacterial cytoplasm. Complementation with and , which map downstream of , restored motility to some of the mutants which became flagellated. After inactivation of either or , such complementation no longer occurred and the flagellin protein accumulated in the cytoplasm, which suggested that both genes are required for the secretion of flagellin and expression of motility. Expression of these genes from high copy number plasmids resulted in the synthesis of exceptionally long flagella and in detection of the FliV protein on polyacrylamide gels.

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1993-10-01
2021-04-21
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