1887

Abstract

SUMMARY: Two new segregants, PPW1-1 and PPW161-1, of were isolated from the stock cultures PaW85(pWW0) and PaW85(pWW0-161). Strain PPW1-1 had lost its ability to grow on -xylene but was able to grow on -toluate. A deletion of the left-hand of transposon Tn, including the -operon genes, had taken place in plasmid pWWO11, isolated from strain PPW1-1. Additional deletions were observed in pWWO after ‘benzoate-curing’ (plasmids pWW0, pWWO, pWW0). The genes of the -operon and beginning of the -operon were deleted from pWW0-161, isolated from strain PPW161-1. Despite this deletion, cells of PPW161-1 grew on all normal TOL plasmid substrates. The Tol phenotype was stable in cells of PPW161-1 growing on benzoate. We propose that this is because in cells of strain PPW161-1 the catabolic genes deleted from pWW0-161 were integrated into the chromosome at the site where the (chromosomally encoded) -pathway genes are located, resulting in the inability of the cells to use this pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-10-2379
1993-10-01
2019-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-10-2379
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error