1887

Abstract

Previously reported cell fractionation experiments have yielded conflicting information on the cellular localization of the DnaK heat shock protein of . Here we used immunogold labelling of ultra-thin sections to determine the localization of DnaK in unstressed cells at 30 °C as well as in heat-shocked cells. In cells grown at 30 °C, gold particles were found predominantly in the cytoplasm, indicating that the majority of the DnaK molecules are cytoplasmic; however, a fraction of the gold particles was located in proximity to the membranes, raising the possibility that a subpopulation of DnaK proteins is membrane-associated. Heat shock of the cells did not induce detectable relocalization of DnaK.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-1-95
1993-01-01
2019-10-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-1-95
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error