By selecting for growth of mutant strains in the absence of the required amino acid, clones were found in a library carrying genes for glutamylphosphate reductase () and ß-isopropylmalate dehydrogenase (). These clones hybridized to unique fragments in a genomic digest of DNA. The -complementing DNA was found in a region of 1.3 kb, which directed the synthesis of a protein of 48000 Da with a pI of 6.3 in maxicells. The -complementing activity was in a region of 1.4 kb and directed synthesis of a protein of 46000 Da with a pI of 5.9.


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