1887

Abstract

By selecting for growth of mutant strains in the absence of the required amino acid, clones were found in a library carrying genes for glutamylphosphate reductase () and -isopropylmalate dehydrogenase (). These clones hybridized to unique fragments in a genomic digest of DNA. The -complementing DNA was found in a region of 1·3 kb, which directed the synthesis of a protein of 48000 Da with a pI of 6·3 in maxicells. The -complementing activity was in a region of 1·4 kb and directed synthesis of a protein of 46000 Da with a pI of 5·9.

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1993-01-01
2024-04-25
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