1887

Abstract

By selecting for growth of mutant strains in the absence of the required amino acid, clones were found in a library carrying genes for glutamylphosphate reductase () and -isopropylmalate dehydrogenase (). These clones hybridized to unique fragments in a genomic digest of DNA. The -complementing DNA was found in a region of 1·3 kb, which directed the synthesis of a protein of 48000 Da with a pI of 6·3 in maxicells. The -complementing activity was in a region of 1·4 kb and directed synthesis of a protein of 46000 Da with a pI of 5·9.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-1-87
1993-01-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/1/mic-139-1-87.html?itemId=/content/journal/micro/10.1099/00221287-139-1-87&mimeType=html&fmt=ahah

References

  1. Ames G.F.-L., Nikaido K. 1976; Two-dimensional gel electro-phoresis of membrane proteins. Biochemistry 15:616–622
    [Google Scholar]
  2. Bacon G.A., Burrows T.W., Yates M. 1951; The effects of biochemical mutation on the virulence of Bacterium typhosum: the virulence of mutants. British Journal of Experimental Pathology 31:714–724
    [Google Scholar]
  3. Berg C.M., Curtiss R.III 1967; Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics 56:503–525
    [Google Scholar]
  4. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Bricker B.J., Tabatabai L.B., Judge B.A., Deyoe B.L., Mayfield J.E. 1990; Cloning, expression and occurrence of the Brucella Cu-Zn superoxide dismutase. Infection and Immunity 58:2935–2939
    [Google Scholar]
  6. Broda P., Meacock P., Achtman M. 1972; Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Molecular and General Genetics 116:336–347
    [Google Scholar]
  7. Carle G.F., Frank M., Olson M.V. 1986; Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 232:65–68
    [Google Scholar]
  8. Chomczynski P., Qasba P.K. 1984; Alkaline transfer of DNA to plastic membrane. Biochemical and Biophysical Research Communications 122:340–344
    [Google Scholar]
  9. Chung C.T., Niemela S.L., Miller R.H. 1989; One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences of the United States of America 86:2172–2175
    [Google Scholar]
  10. Csonka L.N., Baich A. 1983; Proline biosynthesis. In Amino Acids: Biosynthesis and Genetic Regulation, pp. 35–51, Edited By. Herrmann. K.M. R L.Somerville.Addison-Wesley.; Reading MA::
    [Google Scholar]
  11. Csonka L.N., Clark A.J. 1979; Deletions generated by the transposon Tn 10 in the srl recA region of the Escherichia coli K12 chromosome. Genetics 93:321–343
    [Google Scholar]
  12. Deutch A.H., Rushlow K.E., Smith C.J. 1984; Analysis of the Escherichia coli proAB locus by DNA and protein sequencing. Nucleic Acids Research 12:6337–6355
    [Google Scholar]
  13. Ficht T.A., Bearden S.W., Sowa B.A., Adams L.G. 1988; A 36-kilodalton Brucella abortus cell envelope protein is encoded by repeated sequences closely linked in the genomic DNA. Infection and Immunity 56:2036–2046
    [Google Scholar]
  14. Forster A.C., Mcinnes J.L., Skingle D.C., Symons R.H. 1985; Non-radioactive hybridization probe prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acids Research 13:745–761
    [Google Scholar]
  15. Gerhardt P. 1958; The nutrition of Brucellae. Bacteriological Reviews 22:81–98
    [Google Scholar]
  16. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  17. Hayashi S., Koch J.P., Lin E.C.C. 1964; Active transport of L-α-glycerol phosphate in Escherichia coli. Journal of Biological Chemistry 239:3098–3105
    [Google Scholar]
  18. Hayzer D.J., Leisinger T. 1980; The gene-enzyme relationships of proline biosynthesis in Escherichia coli.. Journal of General Microbiology 118:287–293
    [Google Scholar]
  19. Hayzer D.J., Leisinger T. 1982; Proline biosynthesis in Escherichia coli. Purification and characterization of glutamate semialdehyde dehydrogenase. European Journal of Biochemistry 121:561–565
    [Google Scholar]
  20. Heinrich P. 1988; Construction of nested deletions for DNA sequencing. In Current Protocols in Molecular Biology, I, Supplement 2, pp. 1-7.3.20. , Edited by. Ausubel F. M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. New York:: John Wiley;
    [Google Scholar]
  21. Imai R., Sakiguchi T., Nosoh Y., Tsuda K. 1987; The nucleotide sequence of 3-isopropylmalate dehydrogenase gene from Bacillus subtilis. Nucleic Acids Research 15:4988
    [Google Scholar]
  22. Ish-horowicz D., Burke J.F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  23. Jackson D.A., Cook P.R. 1985; A general method for preparing chromatin containing intact DNA. EMBO Journal 4:913–918
    [Google Scholar]
  24. Kirino H., Oshima T. 1991; Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile, Thermus aquaticus YT-1. Journal of Biochemistry (Japan) 109:852–857
    [Google Scholar]
  25. Kushner S.R., Nagaishi H., Templin A., Clark A.J. 1971; Genetic recombination in Escherichia coli: the role of exonuclease I. Proceedings of the National Academy of Sciences of the United States of America 68:824–827
    [Google Scholar]
  26. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  27. Leisinger T. 1987; Biosynthesis of proline. In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology,. 1 pp. 345–351, Edited by. Neidhardt F.C. Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  28. Luria S.E., Burrous J.W. 1957; Hybridization between Escherichia coli and Shigella.. Journal of Bacteriology 97:461–476
    [Google Scholar]
  29. Maloy S.R., Nunn W.D. 1981; Selection for loss of tetracycline resistance by Escherichia coli.. Journal of Bacteriology 145:1110–1112
    [Google Scholar]
  30. Mayfield J.E., Bricker B.J., Godfrey H., Crosby R.M., Knight D.J., Halling S.M., Balinsky D., Tabatabai L.B. 1988; The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein.. Gene 63:1–9
    [Google Scholar]
  31. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory:
    [Google Scholar]
  32. Murray J.A.H. 1986; HCC ligation: rapid and specific DNA construction with blunt ended DNA fragments. Nucleic Acids Research 14:10118
    [Google Scholar]
  33. Omori K., Suzuki S.I., Imai Y., Komatsubara S. 1991; Analysis of the Serratia marcescens proAB operon and feedback control of proline biosynthesis. Journal of General Microbiology 137:509–517
    [Google Scholar]
  34. Parsons S.J., Burns R.O. 1969; Purification and properties of β- isopropylmalate dehydrogenase. Journal of Biological Chemistry 244:996–1003
    [Google Scholar]
  35. Sancar A., Rupert C.S. 1978; Correction for the map location for the phr gene in Escherichia coli K12. Mutation Research 51:139–143
    [Google Scholar]
  36. Sancar A., Wharton R.P., Seltzer S., Kacinsky B.M., Clarke N.D., Rupp W.D. 1981; Identification of the uvr A gene product. Journal of Molecular Biology 148:45–62
    [Google Scholar]
  37. Searles L.L., Calvo J.M. 1988; Permeabilized cell and radiochemical assays for βisopropylmalate dehydrogenase. Methods in Enzymology 166:225–229
    [Google Scholar]
  38. Sekiguchi T., Ortega-cesena J., Nosoh Y., Ohashi S., Tsuda K., Kanaya S. 1986; DNA and amino-acid sequences of 3-isopropylmalate dehydrogenase of Bacillus coagulans. Comparison with the enzymes of Saccharomyces cerevisiae and Thermus thermo-philus. Biochimica et Biophysica Acta 867:36–44
    [Google Scholar]
  39. Sekiguchi T., Suda M., Ishii T., Nosoh Y., Tsuda K. 1987; The nucleotide sequence of 3-isopropylmalate dehydrogenase gene from Bacillus caldotenax.. Nucleic Acids Research 15:853
    [Google Scholar]
  40. Smith C.L., Cantor C.R. 1987; Purification, specific fragmentation, and separation of large DNA molecules. Methods in Enzymology 155:449–467
    [Google Scholar]
  41. Somers J.M., Amzallag A., Middleton R.B. 1973; Genetic fine structure of the leucine operon of Escherichia coli K-12. Journal of Bacteriology 113:1268–1272
    [Google Scholar]
  42. Stieglitz B.I., Calvo J.M. 1974; Distribution of the isopropylmalate pathway to leucine among diverse bacteria. Journal of Bacteriology 118:935–941
    [Google Scholar]
  43. Umbarger H.E. 1987; Biosynthesis of the branched-chain amino acids. In Escherichia coli and Salmonella typhimurium.. Cellular and Molecular Biology, 1 pp. 352–367, Edited by. Neidhardt F.C. Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  44. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7- derived system for insertional mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  45. Williams I., Frank L. 1975; Improved chemical synthesis and enzymatic assay of △1-pyrroline-5-carboxylic acid. Analytical Bio-chemistry 64:85–97
    [Google Scholar]
  46. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-1-87
Loading
/content/journal/micro/10.1099/00221287-139-1-87
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error