
Full text loading...
By selecting for growth of Escherichia coli mutant strains in the absence of the required amino acid, clones were found in a Brucella abortus library carrying genes for glutamylphosphate reductase (proA) and β-isopropylmalate dehydrogenase (leuB). These clones hybridized to unique fragments in a genomic digest of B. abortus DNA. The proA-complementing DNA was found in a region of 1·3 kb, which directed the synthesis of a protein of 48000 Da with a pI of 6·3 in maxicells. The leuB-complementing activity was in a region of 1·4 kb and directed synthesis of a protein of 46000 Da with a pI of 5·9.
Article metrics loading...
Full text loading...
References
Data & Media loading...