1887

Abstract

The gene, encoding a -1,4-endoglucanase (EG), was shown to be a useful reporter gene for the study of gene expression in . The reporter gene has the advantages that it can be easily identified in both and on agar media containing carboxymethylcellulose, and EG production can be rapidly quantified in liquid medium. Since the glutamine synthetase (GS) is inactivated in permeabilized cells and cell extracts, the reporter gene was used to study the regulation of GS production in . Gene fusions containing the GS promoter region fused to the promoterless gene showed that expression was regulated by nitrogen in at the transcriptional level. A upstream region containing a near-perfect direct repeat sequence was essential for efficient GS expression and for regulation by nitrogen.

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/content/journal/micro/10.1099/00221287-139-1-59
1993-01-01
2021-10-23
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