1887

Abstract

The gene of OE 28.3 was isolated by complementation of the Fec phenotype of recombinantlambda EMBL3 phages in a RecA strain. The subcloned restored resistance to UV and methyl methanesulphonate (MMS) exposure in mutants of . . DNA sequence analysis showed that the coding region of the . gene, specifying a protein of 352 amino acid residues, waspreceded by an SOS box highly similar to those of and . The deduced amino acid sequence displayed highest homology to the RecA proteins from . (87.8% identity) and . (84.3% identity). In both the regulatory region and the structural gene, a relatively high degree of sequence divergence from the gene was observed. A mutant of . was constructed by inserting a kanamycin resistance cassette into its gene. This mutant exhibited an increased sensitivity to UV irradiation and MMS, and was strongly impaired in homologous recombinational activity.

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1993-01-01
2021-04-21
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