1887

Abstract

The gene of OE 28.3 was isolated by complementation of the Fec phenotype of recombinantlambda EMBL3 phages in a RecA strain. The subcloned restored resistance to UV and methyl methanesulphonate (MMS) exposure in mutants of . . DNA sequence analysis showed that the coding region of the . gene, specifying a protein of 352 amino acid residues, waspreceded by an SOS box highly similar to those of and . The deduced amino acid sequence displayed highest homology to the RecA proteins from . (87.8% identity) and . (84.3% identity). In both the regulatory region and the structural gene, a relatively high degree of sequence divergence from the gene was observed. A mutant of . was constructed by inserting a kanamycin resistance cassette into its gene. This mutant exhibited an increased sensitivity to UV irradiation and MMS, and was strongly impaired in homologous recombinational activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-1-49
1993-01-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/1/mic-139-1-49.html?itemId=/content/journal/micro/10.1099/00221287-139-1-49&mimeType=html&fmt=ahah

References

  1. Bairoch A. 1991; PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Research 19:2241–2245
    [Google Scholar]
  2. Barbé J., Fernandez de Henestrosa A. R., Calero S., Gibert I. 1991; Chromogenic method for rapid isolation of recA- like mutants of Gram-negative bacteria. Journal of Bacteriology 173:404–406
    [Google Scholar]
  3. Calero S., Garriga X., Barbé J. 1991; One step cloning system for isolation of bacterial lex A-like genes. Journal of Bacteriology 173:7345–7350
    [Google Scholar]
  4. Cheo D. L., Bayles K. W., Yasbin R. E. 1991; Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis. Journal of Bacteriology 173:1696–1703
    [Google Scholar]
  5. Davis E. O., Sedgwick S. G., Colston M. J. 1991; Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. Journal of Bacteriology 173:5653–5662
    [Google Scholar]
  6. De Mot R., Vanderleyden J. 1991; Purification of a root- adhesive outer membrane protein of root-colonizing Pseudomonas fluorescens. FEMS Microbiology Letters 81:323–328
    [Google Scholar]
  7. De Mot R., Proost P., Van Damme J., Vanderleyden J. 1992; Homology of the root adhesin of Pseudomonas fluorescens OE 28·3 with porin F of P. aeruginosa and P. syringae. Molecular and General Genetics 231:489–493
    [Google Scholar]
  8. Deretic V., Konyecsni W. M., Mohr C. D., Martin D. W., Hibler N. S. 1989; Common denominators of promoter control in Pseudomonas and other bacteria. Biotechnology 7:1249–1254
    [Google Scholar]
  9. Duwat P., Ehrlich S. D., Gruss A. 1992; A general method for cloning recA genes of Gram-positive bacteria by polymerase chain reaction. Journal of Bacteriology 174:5171–5175
    [Google Scholar]
  10. Dybvig K., Woodard A. 1992; Cloning and DNA sequence of a mycoplasmal recA gene. Journal of Bacteriology 174:778–784
    [Google Scholar]
  11. Dybvig K., Hollingshead S. K., Heath D. G., Clewell D. B., Sun F., Woodard A. 1992; Degenerate oligonucleotide primers for enzymatic amplifications of recA sequences from Gram-positive bacteria and mycoplasmas. Journal of Bacteriology 174:2729–2732
    [Google Scholar]
  12. Favre D., Viret J.-F. 1990; Nucleotide sequence of the recA gene of Bordetella pertussis. Nucleic Acids Research 18:4243
    [Google Scholar]
  13. Fernandez de Henestrosa A. R., Calero S., Barbé J. 1991; Expression of the recA gene of Escherichia coli in several species of Gram-negative bacteria. Molecular and General Genetics 226:503–506
    [Google Scholar]
  14. Frischauf A. M., Murray N., Lehrach H. 1987; Lambda phage vectors - EMBL series. Methods in Enzymology 153:103–115
    [Google Scholar]
  15. Früh R., Watson J. M., Haas D. 1983; Construction of recombination-deficient strains of Pseudomonas aeruginosa. Molecular and General Genetics 191:334–337
    [Google Scholar]
  16. Fyfe J. A.M., Davies J. K. 1990; Nucleotide sequence and expression in Escherichia coli of the recA gene of Neisseria gonorrhoeae. Gene 93:151–156
    [Google Scholar]
  17. Ghosh S. K., Biswas S. K., Paul K., Das J. 1992; Nucleotide and deduced amino acid sequence of the recA gene of Vibrio cholerae. Nucleic Acids Research 20:372
    [Google Scholar]
  18. Gomelsky M., Gak E., Chistoserdov A., Bolotin A., Tsygankov Y. D. 1990; Cloning, sequence and expression in Escherichia coli of the Methylobacillus flagellatum recA gene. Gene 94:69–75
    [Google Scholar]
  19. Goodman H. J.K., Woods D. R. 1990; Molecular analysis of the Bacteroides fragilis recA gene. Gene 94:77–82
    [Google Scholar]
  20. Higgins C. F., Hiles I. D., Whalley K., Jamieson D. J. 1985; Nucleotide binding by membrane components of bacterial peri- plasmic binding protein-dependent transport systems. EMBO Journal 4:1033–1040
    [Google Scholar]
  21. Lockett T. J. 1990; A bacteriophage lambda DNA purification procedure suitable for the analysis of DNA from either large or multiple small lysates. Analytical Biochemistry 185:230–234
    [Google Scholar]
  22. Lugtenberg B. J.J., de Weger L. A., Bennett J. W. 1991; Microbial stimulation of plant growth and protection from disease. Current Opinion in Biotechnology 2:457–464
    [Google Scholar]
  23. MartÍnez-Salazar J. M., Romero D., de Lourdes Girard M., Davila G. 1991; Molecular cloning and characterization of the recA gene of Rhizobium phaseoli and construction of recA mutants. Journal of Bacteriology 173:3035–3040
    [Google Scholar]
  24. Michiels J., Vande Broek A., Vanderleyden J. 1991; Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recAgene. Molecular and General Genetics 228:486–490
    [Google Scholar]
  25. Miller R. V., Kokjohn T. A. 1990; General microbiology of recA : environmental and evolutionary significance. Annual Review of Microbiology 44:365–394
    [Google Scholar]
  26. Nakazawa T., Kimoto M., Abe M. 1990; Cloning, sequencing and transcriptional analysis of the recA gene of Pseudomonas cepacia. Gene 94:83–88
    [Google Scholar]
  27. Roca A. I., Cox M. M. 1990; The recA protein: structure and function. CRC Critical Reviews in Biochemistry and Molecular Biology 25:415–456
    [Google Scholar]
  28. Sano Y., Kageyama M. 1987; The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO. Molecular and General Genetics 208:412–419
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual, 2nd edn Cold Spring Harbour, NY: Cold Spring Harbor Laboratory.:
    [Google Scholar]
  30. Selbitschka W., Arnold W., Priefer U. B., RottschÄFER T., Schmidt M., Simon R., PÙhler A. 1991; Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae. Molecular and General Genetics 229:86–95
    [Google Scholar]
  31. Simon R., Priefer U., PÙhler A. 1983; A broad host range mobilization system for in vivo engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1:748–751
    [Google Scholar]
  32. Tolmasky M. E., Gammie A. E., Crosa J. H. 1992; Characterization of the recA gene of Vibrio anguillarum. Gene 110:41–48
    [Google Scholar]
  33. Venkatesh T. V., Das H. K. 1992; The Azotobacter vinelandii recAgene: sequence analysis and regulation of expression. Gene 113:47–53
    [Google Scholar]
  34. Walker G. C. 1987; The SOS response of Escherichia coli. In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology, 2 pp. 1346–1357 Neidhardt F. C., Ingraha Low J. L. K. B., B., Schaechter M., Umbarger H. E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  35. Willis D. K., Hrabak E. M., Lindow S. E., Panopoulos N. J. 1988; Construction and characterization of Pseudomonas syringae recA mutant strains. Molecular Plant-Microbe Interactions 1:80–86
    [Google Scholar]
  36. Zhao X. J., McEntee K. 1990; DNA sequence analysis of the recAgenes from Proteus vulgaris, Erwinia carotovora, Shigellaflexneriand Escherichia coli B/r. Molecular and General Genetics 222.:369–376
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-1-49
Loading
/content/journal/micro/10.1099/00221287-139-1-49
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error