Flagellin from subspecies strain Bt75 was isolated both from the culture medium and from flagella. Two protein forms with molecular masses close to 32 kDa were obtained from flagella; one form was identical to the flagellin purified from the culture medium. The N-terminal amino acid sequences were identical for both forms. Two genes coding for flagellin have been identified in subsp. . The gene was cloned and sequenced in its entire length. The clone containing the gene was incomplete. Both genes were expressed in the mid-exponential growth phase. The gene was flanked by long (355 bp) direct repeats protruding into the coding region in both the N- and C-terminal parts of the gene. DNA sequences related to the gene were found in most other subspecies, and in two of them, subsp. and subsp. , such sequences were present in multiple copies.


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