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Volume 139, Issue 1

Identification of two expressed flagellin genes in the insect pathogen subsp. alesti Free

Abstract

Flagellin from subspecies strain Bt75 was isolated both from the culture medium and from flagella. Two protein forms with molecular masses close to 32 kDa were obtained fromflagella; one form was identical to the flagellin purified from the culture medium. The N-terminal amino acid sequences were identical for both forms. Two genes coding for flagellin have been identified in subsp. . The gene was cloned and sequenced in its entire length. The clone containing the gene was incomplete. Both genes were expressed in the mid-exponential growth phase.The gene was flanked by long (355 bp) direct repeats protruding into the coding region in both th N- and C-terminal parts of the gene. DNA sequences related to the gene were found in most other subspecies, and in two of them, subsp. and subsp. , such sequences were present in multiple copies.

  • Received:
  • Accepted:
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  • Published Online:
© Society for General Microbiology, 1993
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1993-01-01
2024-04-23
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Identification of two expressed flagellin genes in the insect pathogen Bacillus thuringiensissubsp. alesti
Microbiology 139, 21 (1993); https://doi.org/10.1099/00221287-139-1-21
/content/journal/micro/10.1099/00221287-139-1-21
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