1887

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Volume 138, Issue 9

Extracellular polygalacturonases from : Separation and regulatory aspects Free

Abstract

Extracellular polygalacturonase activities of were induced by pectin and repressed, but not inactivated, by glucose. The absence of a carbohydrate source derepressed 50% of viscosity-diminishing activity and 36% of reducing-groups-releasing activity compared to production in the presence of pectin. High concentrations (30 m and 50 m) of -galacturonic acid reduced only the viscosity-diminishing activity, and under these conditions the fungus grew poorly. Neither effect was observed if the mycelium had been previously induced by pectin. Polygalacturonase activities produced in the presence of pectin were separated by ion exchange chromatography. These enzymes eluted in six peaks, characterized as exopolygalacturonases I, II and III and endopolygalacturonases, I, II and III.

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© Society for General Microbiology, 1992
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1992-09-01
2024-04-19
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References

  1. Aguilar C., Huitron C. 1987; Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition. Enzyme and Microbial Technology 9:690–696
     [Google Scholar]
  2. Aguilar C., Huitron C. 1990; Constitutive exo-pectinase produced by Aspergillus sp. CH-Y-1043 on different carbon source. Biotechnology Letters 12:655–660
     [Google Scholar]
  3. Albersheim P. 1966; Pectin lyase from fungi. Methods in Enzymology 8:628–631
     [Google Scholar]
  4. Fonseca M. J. V., Spadaro A. C. C., Said S. 1991; Separation of the components of the pectolytic complex produced by Tubercularia vulgaris in solid state culture. Biotechnology Letters 13:39–42
     [Google Scholar]
  5. Leone G., Van den Heuvel J. 1987; Regulation by carbohydrates of the sequential ‘in vitro’ production of pectic enzymes by Botrytis cinerea. Canadian Journal of Botany 65:2133–2141
     [Google Scholar]
  6. Manachini P. L., Parini C., Fortina M. G. 1988; Pectic enzymes from Aureobasidium pullullans LV 10. Enzyme and Microbial Technology 10:682–685
     [Google Scholar]
  7. Mill P. J., Tuttobello R. 1961; The pectic enzymes of Aspergillus niger. II: Endopolygalacturonase. Biochemical Journal 79:57–64
     [Google Scholar]
  8. Miller G. L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Biochemistry 31:426–428
     [Google Scholar]
  9. Rombouts F. M., Pilnik W. 1980; Pectic enzymes. In Economic Microbiology vol. 5 pp. 227–282 Edited by Rose A. H. Academic Press; London:
     [Google Scholar]
  10. Said S., Fonseca M. J. V., SiÉssere V. 1991; Pectinase production by Penicillium frequentans. World Journal of Microbiology and Biotechnology 7:607–608
     [Google Scholar]
  11. Strzelczyk E., Dahm H., Kowalski S. 1987; The influence of pH and temperature on cellulolytic and pectolytic activity of Cylindrocarpon destructans (Zins) Scholt. Journal of Phytopathology 120:97–103
     [Google Scholar]
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Extracellular polygalacturonases from Penicillium frequentans: Separation and regulatory aspects
Microbiology 138, 1801 (1992); https://doi.org/10.1099/00221287-138-9-1801
/content/journal/micro/10.1099/00221287-138-9-1801
/content/journal/micro/10.1099/00221287-138-9-1801
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