1887

Abstract

Twenty-eight non-spreading mutants of the gliding bacterium were generated by different approaches and subsequently studied to determine whether they possessed traits commonly assumed to be associated with such mutants. Many non-spreading variants of purportedly possess a static cell surface as opposed to the dynamic one of the motile parent strain. A moving cell surface has been supposed to be responsible for an array of traits associated with motile cells, but missing in non-motile cells. Phage sensitivity, chitin digestion and the ability of cells to move latex beads over their surfaces are some of the alleged motility-dependent traits. Also, it has been reported that non-spreading mutants possess a cell surface that is less hydrophobic than that of the parent strain. We characterized our collection of mutants in relation to the above mentioned traits to determine whether these characteristics indeed required a moving cell surface. Our findings showed that neither phage sensitivity nor chitin digestion were motility-dependent. In addition we noted that non-spreading mutants could possess surfaces more (rather than less) hydrophobic than the motile parent strain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1767
1992-08-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1767.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1767&mimeType=html&fmt=ahah

References

  1. Abbanat D. R., Leadbetter E., III R, Godchaux W., Escher A. 1986; Sulphonolipids are molecular determinants of gliding motility. Nature, London 324:367–369
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry 12:248–254
    [Google Scholar]
  3. Chang L.-Y. E., Pate J. L., Betzig R. F. 1984; Isolation and characterization of nonspreading mutants of the gliding bacterium Cytophaga johnsonae . Journal of Bacteriology 159:26–35
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. Journal of Cellular and Comparative Physiology 49:25–68
    [Google Scholar]
  5. III Godchaux W., Leadbetter E. R. 1983; Unusual sulfonolipids are characteristic of the Cytophaga-Flexibacter group of gliding bacteria. Journal of Bacteriology 153:1238–1246
    [Google Scholar]
  6. III Godchaux W., Gorski L., Leadbetter E. R. 1990; Outer membrane polysaccharide deficiency in two nongliding mutants of Cytophaga johnsonae . Journal of Bacteriology 172:1250–1255
    [Google Scholar]
  7. III Godchaux W., Lynes M. A., Leadbetter E. R. 1991; Defects in gliding motility in mutants of Cytophaga johnsonae lacking a high molecular weight cell-surface polysaccharide. Journal of Bacteriology 173:7607–7614
    [Google Scholar]
  8. Gorski L., Leadbetter E. R., III Godchaux W. 1991; Temporal sequence of trait recovery during phenotypic curing of a Cytophaga johnsonae motility mutant. Journal of Bacteriology 173:7534–7539
    [Google Scholar]
  9. Lapidus I. R., Berg H. C. 1982; Gliding motility of Cytophaga sp. strain U-67. Journal of Bacteriology 151:384–398
    [Google Scholar]
  10. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Pate J. L. 1988; Gliding motility in prokaryotes. Canadian Journal of Microbiology 34:459–465
    [Google Scholar]
  12. Pate J. L., Chang L.-Y. E. 1979; Evidence that gliding motility in prokaryotic cells is driven by rotary assemblies in the cell envelopes. Current Microbiology 2:59–64
    [Google Scholar]
  13. Pate J. L., Petzold S. J., Chang L.-Y. E. 1979; Phages for the gliding bacterium Cytophaga johnsonae that infect only motile cells. Current Microbiology 2:257–262
    [Google Scholar]
  14. Pitta T. P., III Godchaux W., Leadbetter E. R. 1989; Increase in ornithine amino lipid content in a sulfonolipid-deficient mutant of Cytophaga johnsonae . Journal of Bacteriology 171:952–957
    [Google Scholar]
  15. Sorongon M. L., Bloodgood R. A., Burchard R. P. 1991; Hydrophobicity, adhesion, and surface-exposed proteins of gliding bacteria. Applied and Environmental Microbiology 57:3193–3199
    [Google Scholar]
  16. Stanier R. Y. 1946; Studies on non-fruiting myxobacterium. 1.Cytophaga johnsonae sp., a chitin decomposing myxobacterium. Journal of Bacteriology 53:297–315
    [Google Scholar]
  17. Wolkin R. H., Pate J. L. 1985; Selection for nonadherent or nonhydrophobic mutants co-selects for nonspreading mutants of Cytophaga johnsonae and other gliding bacteria. Journal of General Microbiology 131:737–750
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1767
Loading
/content/journal/micro/10.1099/00221287-138-8-1767
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error