1887

Abstract

The functioning of the alanine/-alanyl--alanine pathway of was investigated by determining prefnsor pool levels and specific enzyme activities under various growth conditions. Cells grown on - or -alanine showed several remarkable features compared with cells grown on other carbon sources: 10-fold higher values of the -alanyl--alanine and the UDP-MurNAc-pentapeptide pools, a 240-fold increase of the alanine racemase activity, and the absence of bacteriolysis after treatment with -cycloserine at high concentrations (50 μg ml). In cells grown on glucose, -cycloserine (1 μg ml) led to depletion of the -alanyl--alanine pool and to lysis, which was efficiently antagonized by chloramphenicol. A threefold increase of the dipeptide pool was observed when cells were treated with chloramphenicol alone. The alanine racemase activity was lowest in glucose-grown cells and the -alanine: -alanine ligase and -alanyl--alanine-adding activities were the same whatever the carbon source. Molecular masses of 53–56 kDa and 56–60 kDa were estimated for the partially purified inducible alanine racemase and -alanine: -alanine ligase respectively.

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1992-08-01
2021-05-16
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