Cloning and DNA sequence analysis of a bacteriocin gene of Free

Abstract

N28b synthesized and secreted a bacteriocin, with a molecular mass of 45 kDa, which was capable of inhibiting the growth of . The expression of this bacteriocin was negligible unless induced with mitomycin C. The genes encoding the bacteriocin were cloned in plasmid pBR328. harbouring recombinant plasmid pBA189 or pBA289 expressed the N28b bacteriocin. The nucleotide sequence of the gene ( N28b bacteriocin structural gene) was determined. The predicted amino acid sequence of the carboxy-terminal part of the bacteriocin 28b had a high degree of similarity to the pore-forming domains of colicins A, E1, B, N, Ia and Ib.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1737
1992-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1737.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1737&mimeType=html&fmt=ahah

References

  1. Bullock W O., Fernandez J. M., Short J. M. 1987; XLl-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase section. Biotechniques 5:376–379
    [Google Scholar]
  2. Corpet F. 1988; Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16:10881–10890
    [Google Scholar]
  3. De Graaf F. K., Oudega B. 1986; Production and release of cloacin DF13 and related colicins. Current Topics in Microbiology and Immunology 125:183–205
    [Google Scholar]
  4. Eisenberg D., Schwarz E., Komaromy M., Wall R. 1984; Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Journal of Molecular Biology 179:125–142
    [Google Scholar]
  5. Foulds J. 1972; Purification and partial characterization of a bacteriocin from Serratia marcescens . Journal of Bacteriology 110:1001–1009
    [Google Scholar]
  6. Foulds J., Shemin D. 1969; Properties and characteristics of a bacteriocin from Serratia marcescens . Journal of Bacteriology 99:655–660
    [Google Scholar]
  7. Gargallo-Viola D. V. 1989; Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens . Journal of Clinical Microbiology 27:860–868
    [Google Scholar]
  8. Juarez A., Hartlein M., Goebel W. 1984; Study of regulation and transport of hemolysin by using fusion of β-galactosidase gene (lacZ) to hemolysin genes. Journal of Bacteriology 160:161–168
    [Google Scholar]
  9. Lacks A. S., Springhorn S. S. 1980; Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Journal of Biological Chemistry 255:7467–7473
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning . A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Oliver D. 1985; Protein secretion in Escherichia coli . Annual Review of Microbiology 39:615–644
    [Google Scholar]
  13. Pattus F., Massotte D., Wilmsen H. U., Lakey D., Tsernoglou D., Tucker A., Parker M. W. 1990; Colicins: prokaryotic killer-pores. Experientia 46:180–192
    [Google Scholar]
  14. Pugsley A. P. 1988; Protein secretion across the outer membrane of Gram-negative bacteria. In Protein Transfer and Organelle Biogenesis pp. 607–652 Edited by Das R. C., Robbins P. W. Orlando, Florida: Academic Press Inc;
    [Google Scholar]
  15. Pugsley A. P., Oudega B. 1987; Methods for studying colicins and their plasmids. In Plasmids, a Practical Approach pp. 105–161 Edited by Hardy K. G. Oxford: IRL Press;
    [Google Scholar]
  16. Regué M., Wu H. C. 1988; Synthesis and export of lipoproteins in bacteria. In Protein Transfer and Organelle Biogenesis pp. 587–606 Edited by Das R. C., Robbins P. W. Orlando, Florida: Academic Press;
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  18. Traub W. H. 1980; Bacteriocin and phage typing of Serratia . In The Genus Serratia pp. 79–100 Edited by Graevenitz A., Rubin S. J. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  19. Walker G. C. 1987; The SOS response of Escherichia coli . In Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology vol. 2 pp. 1346–1357 Edited by Neidhardt F. C. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1737
Loading
/content/journal/micro/10.1099/00221287-138-8-1737
Loading

Data & Media loading...

Most cited Most Cited RSS feed