1887

Abstract

An investigation into the induction of the cellulase complex of has shown that crystalline cotton cellulose was effectively degraded by the system induced by scoured cotton but not by the system induced by Solka Floc. A variety of techniques including FPLC, chromatofocusing, SDS-PAGE and isoelectric focusing (IEF) were used to separate and characterize the individual cellobiohydrolases, endoglucanases, cellobiases and glucosidases involved in cellulose degradation. A statistical comparison of the enzymic activities of the differently induced systems after resolution by anion exchange chromatography revealed much lower carboxymethylcellulase activity in one of the pooled fractions from a Solka-Floc-induced preparation, suggesting that one of the endoglucanases is either absent or present at a reduced level. IEF separations indicated that the Solka-Floc-induced system lacked an endoglucanase with an alkaline pI.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1639
1992-08-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1639.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1639&mimeType=html&fmt=ahah

References

  1. Berghem L. E. R., Pettersson L. G., Axio-Fredriksson U. B. 1976; The mechanism of enzymatic cellulose degradation. Purification and some properties of two different 1,4-β-glucan glucano-hydrolases from Trichoderma viride . European Journal of Biochemistry 61:621–630
    [Google Scholar]
  2. Bhikhabhai R., Johansson G., Pettersson G. 1984; Isolation of cellulolytic enzymes from Trichoderma reesei QM 9414. Journal of Applied Biochemistry 6:336–345
    [Google Scholar]
  3. Biely P., Markovic O. 1988; Resolution of glycanases of Trichoderma reesei with respect to cellulose and xylan degradation. Biotechnology Applied Biochemistry 10:99–106
    [Google Scholar]
  4. Bisaria V. S., Ghose T. K. 1981; Biodegradation of cellulosic materials: Substrates, microorganisms, enzymes and products. Enzyme and Microbial Technology 3:90–104
    [Google Scholar]
  5. Bittner D. L., McCleary B. V. 1963; The cupric-phenanthroline chelate in the determination of monosaccharides in whole blood. American Journal of Clinical Pathology 40:423
    [Google Scholar]
  6. Brown M. E., Boston M. S. 1961; Ultra-micro sugar determinations using 2,9-dimethyl-1,10-phenanthroline Hydrochloride (Neocuproine). Diabetes 10:60–62
    [Google Scholar]
  7. Eggins H. O. W., Pugh G. J. F. 1962; Isolation of cellulose-decomposing fungi from the soil. Nature, London 193:94–95
    [Google Scholar]
  8. El Gogary S., Leite A., Crivellaro O., El Dorry H., Eveleigh D. E. 1989; Trichoderma reesei cellulase – from mutants to induction. In Trichoderma reesei Celluloses . Biochemistry, Genetics, Physiology and Application pp. 200–211 Edited by Kubicek C. P., Eveleigh D. E., Esterbauer H., Steiner W., Kubicek-Pranz E. M. London: Royal Society of Chemistry;
    [Google Scholar]
  9. Ellouz S., Durand H., Tiraby G. 1987; Analytical separation of Trichoderma reesei cellulases by ion-exchange Fast Protein Liquid Chromatography. Journal of Chromatography 396:307–317
    [Google Scholar]
  10. Enari T. M., Niku-Paavola M. L., Harju L., Lappalainen A., Nummi M. 1981 Journal of Applied Biochemistry 3:157–163
    [Google Scholar]
  11. Eriksson K. E., Johnsrud S. C. 1982; Mineralization of carbon. In Experimental Microbial Ecology Vol. 8 pp. 134–153 Edited by Burns R. G., Slater J. H. Oxford: Blackwell Scientific;
    [Google Scholar]
  12. Fagerstam L. G., Pettersson L. G. 1979; The cellulolytic complex of Trichoderma reesei QM 9414: An immunological approach. FEBS Letters 98:363–367
    [Google Scholar]
  13. Fagerstam L. G., Pettersson L. G. 1980; The 1,4-β-glucan cellobiohydrolases of Trichoderma reesei QM 9414. A new type of cellulolytic synergism. FEBS Letters 119:97–100
    [Google Scholar]
  14. Gong C.-S., Ladisch M. R., Tsao G. T. 1979; Biosynthesis, purification and mode of action of cellulases of Trichoderma . Advances in Chemistry Series 181:261–287
    [Google Scholar]
  15. HAkansson V., Fagerstam L., Pettersson L., Andersson L. 1978; Purification and characterization of a low molecular weight 1,4-β-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414. Biochimica et Biophysica Acta 524:385–392
    [Google Scholar]
  16. Hayn M., Esterbauer H. 1985; Separation and partial characterisation of Trichoderma reesei cellulase by fast chromatofocusing. Journal of Chromatography 329:379–387
    [Google Scholar]
  17. Henrissat B., Driguez H., Schulein M. 1985; Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Biotechnology 3:722–726
    [Google Scholar]
  18. Kyriacou A., Mackenzie C. R., Neufeld R. J. 1987; Detection and characterization of the specific and non-specific endoglucanases of Trichoderma reesei: evidence demonstrating endoglucanase activity by Cellobiohydrolase II. Enzyme and Microbial Technology 9:25–32
    [Google Scholar]
  19. Nevalainen H., Penttila M., Harkki A., Teeri T., Knowles J. 1989; The molecular biology of Trichoderma and its applications to the expression of both homologous and heterologous genes. In Molecular Industrial Mycology Edited by Leong S. A., Berka R. New York: Marcel Dekker;
    [Google Scholar]
  20. Nummi M., Niku-Paavola M.-L., Enari T. M., Raunio V. 1980; Immuno electrophoretic detection of cellulases. FEBS Letters 113:164–166
    [Google Scholar]
  21. Nummi M., Niku-Paavola M.-L., Lappalainen A., Enari T.-M., Raunio V. 1983; Cellobiohydrolase from Trichoderma reesei . Journal of Biochemistry 215:677–683
    [Google Scholar]
  22. Reese E. T., Siu R. G. H., Levinson H. S. 1950; The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis. Journal of Bacteriology 59:485–497
    [Google Scholar]
  23. Sagar B. F. 1985; Mechanism of cellulase action. In Cellulose and its Derivatives: Chemistry, Biochemistry and Applications pp. 199–207 Edited by Kennedy J. F., Phillips G. O., Wedlock D. J., Williams P. A. Ellis Harwood;
    [Google Scholar]
  24. Shoemaker S. P., Brown R. D. Jr 1978; Enzymic activities of endo-1,4-β-D-glucanases purified from Trichoderma viride . Biochimica et Biophysica Acta 523:133–146
    [Google Scholar]
  25. Sprey B. 1987; Complexity of cellulases from Trichoderma reesei with acidic isoelectric points: A two dimensional gel electrophoretic study using immunoblotting-detection on acid hydrolase complexes. FEMS Microbiology Letters 43:25–32
    [Google Scholar]
  26. Teeri T. T., Salovuori I., Knowles J. 1983; The molecular cloning of the major cellulase gene from Trichoderma reesei . Bio/Technology 1:696–699
    [Google Scholar]
  27. Teeri T. T., Lehtovaara P., Kauppinen P., Salovuoni S., Knowles J. K. C. 1987; Homologous domain in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II. Gene 51:43–52
    [Google Scholar]
  28. Tilbeurgh H., Claeyssens M. 1985; Detection and differentiation of cellulase components using low molecular mass fluorogenic substrates. FEBS Letters 187:283–288
    [Google Scholar]
  29. Ulker A., Sprey B. 1989; Production and characterization of an unglycosylated low molecular weight 1,4-β-glucan-glucanohydrolase of Trichoderma reesei . In Trichoderma reesei Cellulases: Biochemistry, Genetics, Physiology and Application pp. 60–80 Edited by Kubicek C. P., Eveleigh D. E., Esterbauer H., Steiner W., Kubicek-Pranz E. M. London: Royal Society of Chemistry;
    [Google Scholar]
  30. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  31. Wood T. M. 1968; Cellulolytic enzyme system of Trichoderma koningii . Journal of Biochemistry 109:217–227
    [Google Scholar]
  32. Wood T. M. 1969; The cellulase of Fusarium solani . Journal of Biochemistry 115:457–464
    [Google Scholar]
  33. Wood T. M. 1985; Observations and speculations on the complex interactions involved in the solubilization of native cellulose. In Proceedings of the 16th Congress of the Federation of European Biochemical Societies (1978) pp. 251–256 Edited by Yu A. Ovchinnikov. Utrecht: VNU Science Press;
    [Google Scholar]
  34. Wood T. M., McCrae S. I. 1977; Cellulase from Fusarium solani: Purification and properties of the C, component. Carbohydrate Research 57:117–133
    [Google Scholar]
  35. Wood T. M., McCrae S. I. 1978; In Bioconversion of Cellulosic Substances into Energy Chemicals and Microbial Protein . Edited by Ghose T. K. Delhi: I.I.T;
    [Google Scholar]
  36. Wood T. M., McCrae S. I. 1979; Synergism between enzymes involved in the solubilization of native cellulose. Advances in Chemistry Series 181:181–209
    [Google Scholar]
  37. Wood T. M., McCrae S. I., Macfarlane C. C. 1980; The isolation, purification and properties of the cellobiohydrolase component of Penicillium funiculosum cellulase. Journal of Biochemistry 189:51–65
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1639
Loading
/content/journal/micro/10.1099/00221287-138-8-1639
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error