1887

Abstract

The iron-stress-induced genes and have been cloned and sequenced from the marine unicellular cyanobacterium sp. PCC 7002. These genes code for a photosystem II chlorophyll-binding protein and flavodoxin respectively. The genes form a dicistronic operon that is transcriptionally activated under iron-stress conditions to produce an abundant monocistronic message containing and a much less abundant dicistronic message that also contains . The arrangement of these genes, their transcriptional control and the relative abundance of the monocistronic and dicistronic messages produced under iron stress parallels the pattern shown by the freshwater cyanobacterium sp. PCC 7942. The genes for the corresponding proteins found under iron-replete conditions, CP-43 and ferredoxin, have also been cloned and sequenced. Northern blot analysis indicates that both of these genes are constitutively expressed under both iron-stress and iron-replete conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1613
1992-08-01
2021-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1613.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1613&mimeType=html&fmt=ahah

References

  1. Alam J., Whitaker R. A., Krogmann D. W., Curtis S. E. 1986; Isolation and sequence of the gene for ferredoxin I from the cyanobacterium Anabaena sp. PCC 7120. Journal of Bacteriology 168:1265–1271
    [Google Scholar]
  2. Alt J., Morris J., Westoff P., Herrmann R. G. 1984; Nucleotide sequence of the clustered genes for the 44kd chlorophyll a apoprotein and the “3kd”-like protein of the Photosystem II reaction center in the spinach plastid chromosome. Current Genetics 8:597–606
    [Google Scholar]
  3. Bagg A., Neilands J. B. 1987; Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiological Reviews 51:509–518
    [Google Scholar]
  4. Boyer G. L., Gillam A. H., Trick C. 1987; Iron chelation and uptake. In The Cyanobacteria pp. 415–436 Edited by Fay P., Van Baalen C. Amsterdam, New York & Oxford: Elsevier;
    [Google Scholar]
  5. Bricker T. M. 1990; The structure and function of CPa-1 and CPa-2 in Photosystem II. Photosynthesis Research 24:1–13
    [Google Scholar]
  6. Crosa J. H. 1989; Genetics and molecular biology of siderophore-mediated iron transport in bacteria. Microbiological Reviews 53:517–530
    [Google Scholar]
  7. Dale R. M. K., ., McClure B. A., Houchins J. P. 1985; A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing. Application to sequencing the com mitochondrial 18S rRNA. Plasmid 13:31–40
    [Google Scholar]
  8. Drummond M. H. 1985; The base sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its protein product to other flavodoxins. Biochemical Journal 232:891–896
    [Google Scholar]
  9. Fish L. E., Kuck U., Bogorad L. 1985; Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a protein complex of photosystem I. Journal of Biological Chemistry 260:1413–1421
    [Google Scholar]
  10. Gendel S., Straus N. A., Pulleyblank D., Williams J. 1983; A novel shuttle cloning vector for the cyanobacterium Anacystis nidulans . FEMS Microbiology Letters 19:291–294
    [Google Scholar]
  11. Gingrich J. C., Gasparich G. E., Sauer K., Bryant D. A. 1990; Nucleotide sequence and expression of the two genes encoding D2 protein and the single gene encoding the CP43 protein of photosystem II in the cyanobacterium Synechococcus sp. PCC 7002. Photosynthesis Research 24:137–150
    [Google Scholar]
  12. Golden S. S., Brusslan J., Haselkorn R. 1987; Genetic engineering of the cyanobacterial chromosome. Methods in Enzymology 153:215–231
    [Google Scholar]
  13. Guikema J. A., Sherman L. A. 1983; Organization and function of chlorophyll in membranes of cyanobacteria during iron starvation. Plant Physiology 73:250–256
    [Google Scholar]
  14. Hanahan D. 1985; Techniques for transformation of E. coli . In DNA Cloning, a Practical Approach vol. 1 pp. 109–135 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
  15. Hutber G. N., Hutson K. G., J. Rogers L. 1977; Effect of iron deficiency on levels of two ferredoxins and flavodoxin in a cyanobacterium. FEMS Microbiology Letters 1:193–196
    [Google Scholar]
  16. Kjems J., Garrett R. A. 1988; Novel splicing mechanism for the ribosomal RNA intron in the archaebacterium Desulfurococcus mobilis . Cell 54:693–703
    [Google Scholar]
  17. Laudenbach D. E., Straus N. A. 1988; Characterization of a cyanobacterial iron stress-induced gene similar to psbC . Journal of Bacteriology 170:5018–5026
    [Google Scholar]
  18. Laudenbach D. E., Reith M. E., Straus N. A. 1988; Isolation, sequence analysis, and transcriptional studies of the flavodoxin gene from Anacystis nidulans R2. Journal of Bacteriology 170:258–264
    [Google Scholar]
  19. Leonhardt, K G., Straus N. A. 1989; Sequence of flavodoxin from Anabaena variabilis 7120. Nucleic Acids Research 17:4384
    [Google Scholar]
  20. de Lorenzo V., Wee S., Herrero M., Neilands J. B. 1987; Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor. Journal of Bacteriology 169:2624–2630
    [Google Scholar]
  21. Matsubara H., Hase T., Wakabayashi S., Wada K. 1978; Gene duplications during evolution of chloroplast-type ferredoxins. In The Evolution of Protein Molecules pp. 209–219 Edited by Matsubara H., Yamada T. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  22. Milligan B. G. 1989; Purification of chloroplast DNA using hexadecyltrimethylammonium bromide. Plant Molecular Biology Reporter 7:144–149
    [Google Scholar]
  23. Pakrasi H. B., Riethman H. C., Sherman L. A. 1985; Organization of pigment proteins in the photosystem II complex of the cyanobacterium Anacystis nidulans R2. Proceedings of the National Academy of Sciences of the United States of America 82:6903–6907
    [Google Scholar]
  24. Pardo M. B., Gomez-Moreno C., Peleato M. L. 1990; Effect of iron deficiency on ferredoxin levels in Anabaena variabilis PCC 6309. Archives of Microbiology 153:528–530
    [Google Scholar]
  25. Pirrotta V. 1986; Cloning Drosophila genes. In Drosophila: a Practical Approach pp. 83–110 Edited by Roberts D. B. Oxford: IRL Press;
    [Google Scholar]
  26. Reddy K. J., Bullerjahn G. S., Sherman D. M., Sherman L. A. 1987; Cloning, nucleotide sequence, and mutagenesis of a gene (irpA) involved in iron-deficient growth of the cyanobacterium Synechococcus sp. strain PCC7942. Journal of Bacteriology 170:4466–4476
    [Google Scholar]
  27. Reith M. E., Laudenbach D. E., Straus N. A. 1986; Isolation and nucleotide sequence analysis of the ferredoxin I gene from the cyanobacterium Anacystis nidulans R2. Journal of Bacteriology 168:1319–1324
    [Google Scholar]
  28. Riethman H. C., Sherman L. A. 1988a; Immunological characterization of iron-regulated membrane proteins in the cyanobacterium Anacystis nidulans R2. Plant Physiology 88:497–505
    [Google Scholar]
  29. Riethman H. C., Sherman L. A. 1988b; Purification and characterization of an iron stress-induced chlorophyll-protein from the cyanobacterium Anacystis nidulans R2. Biochimica et Biophysica Acta 935:141–151
    [Google Scholar]
  30. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RNA transcription. Annual Review of Genetics 13:319–353
    [Google Scholar]
  31. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd end. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Sandmann G. 1985; Consequences of iron deficiency on photosynthetic electron transport in blue green algae. Photosynthesis Research 6:261–271
    [Google Scholar]
  33. Smith W. W., Pattridge K. A., Ludwig M. L., Petsko G. A., Tsernoglou D., Tanaka N., Yasunobu K. T. 1983; Structure of oxidized flavodoxin from Anacystis nidulans . Journal of Molecular Biology 165:737–755
    [Google Scholar]
  34. Stevens S. E. Jr, Porter R. D. 1980; Transformation in Agmenellum quadruplicatum . Proceedings of the National Academy of Sciences of the United States of America 77:6052–6056
    [Google Scholar]
  35. Vermaas W. F. J., Ikeuchi M. 1991; Photosystem II. In The Photosynthetic Apparatus: Molecular Biology and Operation pp. 25111 Edited by Bogorad L., Vasil I. K. San Diego: Academic Press;
    [Google Scholar]
  36. Vermaas W. F. J., Williams J. G. K., Arntzen C. J. 1987; Sequencing and modification of psbB, the gene encoding the CP-47 protein of photosystem II, in the cyanobacterium Synechocystis 6803. Plant Molecular Biology 8:317–326
    [Google Scholar]
  37. Youvan D. C., Ismail S. 1985; Light-harvesting II (B800–850 complex) structural genes from Rhodopseudomonas capsulata sp. Proceedings of the National Academy of Sciences of the United States of America 82:58–62
    [Google Scholar]
  38. Zuber H. 1986; Structure of light-harvesting antenna complexes of photosynthetic bacteria, cyanobacteria and red algae. Trends in Biochemical Sciences 11:414–419
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1613
Loading
/content/journal/micro/10.1099/00221287-138-8-1613
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error