1887

Abstract

Effects of monovalent cations on luminescence and respiratory activity were studied in the marine luminous bacterium . Maximum oxygen uptake was observed in the presence of Naover the pH range tested (6.5–8.5). At alkaline pH, effects of monovalent cation on luminescence were similar to those on the oxygen uptake. Although KCN addition caused a marked increase in luminescence, the enhanced luminescence with Nawas still greater than that with Li. However, at acidic pH, Kincreases luminescence more than Nadoes. These results indicate that there is not only a competitive but also a cooperative relationship between luminescence and respiration. The respiratory NADH oxidase in the membrane fraction of showed some distinctive characters which are unique to the respiratory-dependent primary Napump, suggesting the possibility of coupling between the Napump and the luciferase system. This was also supported by the results from CCCP-resistant growth and luminescence at alkaline pH. The coupling mechanisms between luminescence and respiration in are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1607
1992-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1607.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1607&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L., Woolkalis M., , & Bang S. 1983; Evolutionary relationships in Vibrio and Photobacterium. A basis for a natural classification. Annual Review of Microbiology 37:369–398
    [Google Scholar]
  2. Grogan D. W. 1984; Interaction of respiration and luminescence in a common marine bacterium. Archives of Microbiology 137:159–162
    [Google Scholar]
  3. Guerrero M. A., , & Makemson J. C. 1989; The cytochromes of luminous bacteria and their coupling to bioluminescence. Current Microbiology 18:67–73
    [Google Scholar]
  4. Hastings J. W., Nealson K. H. 1977; Bacterial bioluminescence. Annual Review of Microbiology 31:549–595
    [Google Scholar]
  5. Kogure K., Tokuda H. 1986; Membrane bioenergetics of halophilic marine bacteria. In Perspectives in Microbial Ecology: Proceedings of the Fourth International Symposium on Microbial Ecology pp. 231–237 Ljubjana:
    [Google Scholar]
  6. Kogure K., Tokuda H. 1989; Respiration-dependent primary Na+ pump in the halophilic marine bacterium, Alcaligenes strain 201. FEBS Letters 256:147–149
    [Google Scholar]
  7. Makemson J. C. 1986; Luciferase-dependent oxygen consumption by bioluminescent vibrios. Journal of Bacteriology 165:461–466
    [Google Scholar]
  8. Meighen E. A. 1988; Enzymes and genes from the lux operons of bioluminescent bacteria. Annual Review of Microbiology 42:151–176
    [Google Scholar]
  9. Meighen E. A. 1991; Molecular biology of bacterial bioluminescence. Microbiological Reviews 55:123–142
    [Google Scholar]
  10. Nealson K. H., Hastings J. W. 1979; Bacterial bioluminescence: its control and ecological significance. Microbiological Reviews 43:496–518
    [Google Scholar]
  11. Nealson K. H., Platt T., Hastings J. W. 1970; Cellular control of the synthesis and activity of the bacterial luminescent system. Journal of Bacteriology 104:313–322
    [Google Scholar]
  12. Tokuda H. 1986; Sodium translocation by NADH oxidase of Vibrio alginolyticus: isolation and characterization of the sodium-pump-defective mutants. Methods in Enzymology 125:520–530
    [Google Scholar]
  13. Tokuda H., Kogure K. 1989; Generalized distribution and common properties of Na+-dependent NADH:quinone oxido-reductases in Gram-negative marine bacteria. Journal of General Microbiology 135:703–709
    [Google Scholar]
  14. Tokuda H., Unemoto T. 1981; A respiration-dependent primary sodium extrusion system functioning at alkaline pH in the marine bacterium Vibrio alginolyticus . Biochemical and Biophysical Research Communications 102:265–271
    [Google Scholar]
  15. Tokuda H., Unemoto T. 1982; Characterization of the respiration-dependent Na+ pump in the marine bacterium Vibrio alginolyticus . Journal of Biological Chemistry 257:10007–10014
    [Google Scholar]
  16. Tokuda H., Unemoto T. 1983; Growth of a marine Vibrio alginolyticus and moderately halophilic V. costicola becomes uncoupler-resistant when the respiration-dependent Na+ pump functions. Journal of Bacteriology 156:636–643
    [Google Scholar]
  17. Tokuda H., Unemoto T. 1984; Na+ is translocated at NADH: quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus . Journal of Biological Chemistry 259:7785–7790
    [Google Scholar]
  18. Ulitzur S., Reinhertz A., Hastings J. W. 1981; Factors affecting the cellular expression of bacterial luciferase. Archives of Microbiology 137:159–162
    [Google Scholar]
  19. Watanabe H., Mimura N., Takimoto A., Nakamura T. 1975; Luminescence and respiratory activities of Photobacterium phosphoreum: Competition for cellular reducing power. Journal of Biochemistry 11:1147–1155
    [Google Scholar]
  20. Watanabe H., Takimoto A., Nakamura T. 1977; Luminescence and respiratory activities of Photobacterium phosphoreum: II. Control by monovalent cations. Journal of Biochemistry 82:1707–1714
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1607
Loading
/content/journal/micro/10.1099/00221287-138-8-1607
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error