RT Journal Article SR Electronic(1) A1 Groenewegen, Peter E. J. A1 Breeuwer, Pieter A1 van Helvoort, Joop M. L. M. A1 Langenhoff, Alette A. M. A1 de Vries, Floris P. A1 de Bont, Jan A. M.YR 1992 T1 Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10 JF Microbiology, VO 138 IS 8 SP 1599 OP 1605 DO https://doi.org/10.1099/00221287-138-8-1599 PB Microbiology Society, SN 1465-2080, AB A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylaminobenzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-8-1599