1887

Abstract

Addition of glucose and other sugars to derepressed cells of the fungus var. triggered activation of the plasma membrane H-ATPase within 5 min. Glucose was the best activator while galactose and lactose had a lesser effect. The activation was not prevented by previous addition of cycloheximide and it was fully reversible when the glucose was removed. The activation process also caused changes in the kinetic properties of the enzyme. The non-activated enzyme had an apparent of about 3.2 m for ATP whereas the activated enzyme showed an apparent of 0.26 m. In addition, the pH optimum of the H-ATPase changed from 6.0 to 7.5 upon activation. The activated enzyme was more sensitive to inhibition by vanadate. When was cultivated in media containing glucose as the major carbon source, enhanced H-ATPase activity was largely confined to the period corresponding to the lag phase, i.e. just before the start of acidification of the medium. This suggests that the activation process might play a role in the onset of extracellular acidification. Addition of glucose to var. cells also caused an increase in the cAMP level. No reliable increase could be demonstrated for the other sugars. Addition of proton ionophores such as DNP and CCCP at pH 5.0 caused both a large increase in the intracellular level of cAMP and in the activity of the plasma membrane H-ATPase. Inhibition of the DNP-induced increase in the cAMP level by acridine orange also resulted in inhibition of the activation of plasma membrane H-ATPase. These results suggest a possible causal relationship between the activity of var. plasma membrane H-ATPase and the intracellular level of cAMP.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-8-1579
1992-08-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/8/mic-138-8-1579.html?itemId=/content/journal/micro/10.1099/00221287-138-8-1579&mimeType=html&fmt=ahah

References

  1. Bisson L. F., Fraenkel D. G. 1984; Expression of kinasedependent glucose uptake in Saccharomyces cerevisiae . Journal of Bacteriology 159:1013–1017
    [Google Scholar]
  2. Bourret J. A. 1986; Evidence that a glucose-mediated rise in cyclic AMP triggers germination of Pilobolus longipes spores. Experimental Mycology 10:60–66
    [Google Scholar]
  3. Brandão R. L., Loureiro-Dias M. C. 1990; Regulation of sugar transport systems in Fusarium oxysporum var. lini . Applied and Environmental Microbiology 56:2417–2420
    [Google Scholar]
  4. Brandão R. L., Nicoli J. R. 1988; Participation des systèmes de captation des carbohydrates et de l’H+-ATPase de la membrane cytoplasmique lors de l’utilisation du glucose et du lactose par Fusarium oxysporum var. lini . Canadian Journal of Microbiology 34:979–986
    [Google Scholar]
  5. Brandao R. L., Nicoli J. R., Figueiredo A. F. S. 1988; Purification and characterization of a β-galactosidase from Fusarium oxysporum var. lini . Journal of Dairy Science 70:1331–1337
    [Google Scholar]
  6. Caspani G., Tortora P., Hanozet G. M., Guerritore A. 1985; Glucose-stimulated cAMP increase may be mediated by intracellular acidification in Saccharomyces cerevisiae . FEBS tetters 186:7579
    [Google Scholar]
  7. Chang A., Slayman C. W. 1991; Maturation of the yeast plasma membrane H+-ATPase involves phosphorylation during intracellular transport. Journal of Cell Biology 115:289–295
    [Google Scholar]
  8. Dewerchin M. A., Van Laere A. J. 1984; Trehalase activity and cyclic AMP content during early development of Mucor rouxii spores. Journal of Bacteriology 158:575–579
    [Google Scholar]
  9. Eraso P., Gancedo C. 1987; Activation of yeast plasma membrane ATPase by acid pH during growth. FEBS Letters 224:187–192
    [Google Scholar]
  10. Fermor T. R., Wood D. A. 1981; Degradation of bacteria by Agaricus bisporus and other fungi. Journal of General Microbiology 126:377–387
    [Google Scholar]
  11. Kolarov J., Kulpa J., Baijot M., Goffeau A. 1988; Characterization of a protein serine kinase from yeast plasma membrane. Journal of Biological Chemistry 263:10613–10619
    [Google Scholar]
  12. McDonough J. P., Mahler H. P. 1982; Covalent phosphorylation of the Mg2+-dependent ATPase of yeast plasma membranes. Journal of Biological Chemistry 257:14579–14581
    [Google Scholar]
  13. Mazon M. J., Gancedo J. M., Gancedo C. 1982; Phosphorylation and inactivation of yeast fructose-biphosphatase in vivo by glucose and by proton ionophores. A possible role for cAMP. European Journal of Biochemistry 127:605–608
    [Google Scholar]
  14. Mazon M. J., Behrens M. M., Portillo F., Pinon R. 1989; cAMP- and /RAS-independent nutritional regulation of plasma-membrane H+-ATPase activity in Saccharomyces cerevisiae . Journal of General Microbiology 135:1453–1460
    [Google Scholar]
  15. Pall M. L. 1977; Cyclic AMP and the plasma membrane potential in Neurospora crassa . Journal of Biological Chemistry 252:7146–7150
    [Google Scholar]
  16. Portillo F., Mazon M. J. 1985; Activation of yeast plasma membrane ATPase by phorbol ester. FEBS Letters 192:95–98
    [Google Scholar]
  17. Portillo F., Mazon M. J. 1986; The Saccharomyces cerevisiae start mutant carrying the cdc25 mutation is defective in activation of plasma membrane ATPase by glucose. Journal of Bacteriology 168:1254–1257
    [Google Scholar]
  18. Purwin C., Nicolay K., Scheffers W. A., Holzer H. 1986; Mechanism of control of adenylate cyclase activity in yeast by fermentable sugars and carbonyl cyanide m-chlorophenylhydrazone. Journal of Biological Chemistry 261:8744–8749
    [Google Scholar]
  19. Serrano R. 1983; In vivo glucose activation of the yeast plasma membrane ATPase. FEBS Letters 156:11–14
    [Google Scholar]
  20. Serrano R. 1985 Plasma Membrane ATPase of Plants and Fungi Boca Raton, Fla.: CRC Press;
    [Google Scholar]
  21. Serrano R. 1988; Structure and function of proton translocating ATPase in plasma membranes of plants and fungi. Biochimica et Biophysica Acta 947:1–28
    [Google Scholar]
  22. Serrano R., Kielland-Brandt M. C., Fink G. R. 1986a; Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+ and Ca++-ATPases. Nature, London 319:689–693
    [Google Scholar]
  23. Serrano R., Montesinos C., Cid A. 1986b; A temperature-sensitive mutant of the yeast plasma membrane ATPase obtained by in vitro mutagenesis. FEBS Letters 208:143–146
    [Google Scholar]
  24. SychrovÁ H., Kotyk A. 1985; Conditions of activation of yeast plasma membrane ATPase. FEBS Letters 183:21–24
    [Google Scholar]
  25. Thevelein J. M. 1984; Cyclic-AMP content and trehalase activation in vegetative cells and ascospores of yeast. Archives of Microbiology 138:64–67
    [Google Scholar]
  26. Thevelein J. M. 1988; Regulation of trehalase activity by phosphorylation–dephosphorylation during developmental transitions in fungi. Experimental Mycology 12:1–12
    [Google Scholar]
  27. Thevelein J. M. 1991; Fermentable sugars and intracellular acidification as specific activators of the RAS-adenylate cyclase signalling pathway in yeast: the relationship to nutrient-induced cell cycle control. Molecular Microbiology 5:1301–1307
    [Google Scholar]
  28. Thevelein J. M., Beullens M. 1985; Cyclic AMP and the stimulation of trehalase activity in the yeast Saccharomyces cerevisiae by carbon sources, nitrogen sources and inhibitors of protein synthesis. Journal of General Microbiology 131:3199–3209
    [Google Scholar]
  29. Thevelein J. M., Beullens M., Honshoven F., Hoebeeck G., Detremerie K., den Hollander J. A., Jans A. W. H. 1987a; Regulation of the cAMP level in the yeast Saccharomyces cerevisiae: intracellular pH and the effect of membrane depolarizing compounds. Journal of General Microbiology 133:2191–2196
    [Google Scholar]
  30. Thevelein J. M., Beullens M., Honshoven F., Hoebeeck G., Detremerie K., Griewel B., den Hollander J. A., Jans A. W. H. 1987b; Regulation of the cAMP level in the yeast Saccharomyces cerevisiae: the glucose-induced cAMP signal is not mediated by a transient drop in the intracellular pH. Journal of General Microbiology 133:2197–2205
    [Google Scholar]
  31. Trevillyan J. M., Pall M. L. 1979; Control of cyclic adenosine 3′,5′-monophosphate levels by depolarizing agents in fungi. Journal of Bacteriology 138:397–403
    [Google Scholar]
  32. Ulaszewski S., Hilger F., Goffeau A. 1989; Cyclic AMP controls the plasma membrane H+-ATPase activity from Saccharomyces cerevisiae . FEBS Letters 245:131–136
    [Google Scholar]
  33. Van der Plaat J. B. 1974; Cyclic 3′-5′-adenosine monophosphate stimulates trehalose degradation in baker’s yeast. Biochemical and Biophysical Research Communications 56:580–587
    [Google Scholar]
  34. Van Mulders R. M., Van Laere A. J. 1984; Cyclic AMP, trehalase and germination of Phycomyces blakesleeanus spores. Journal of General Microbiology 130:541–547
    [Google Scholar]
  35. Vogel H. J. 1956; A convenient growth medium for Neurospora (Medium N). Microbiology General Bulletin 13:42–45
    [Google Scholar]
  36. Yanagita Y., Abdel-Ghany M., Raden D., Nelson N., Racker E. 1987; Polypeptide-dependent protein kinase from baker’s yeast. Proceedings of the National Academy of Sciences of the United States of America 84:925–929
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-8-1579
Loading
/content/journal/micro/10.1099/00221287-138-8-1579
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error