RT Journal Article SR Electronic(1) A1 Harel, Josée A1 Forget, Céline A1 Saint-Amand, Josée A1 Daigle, France A1 Dubreuil, Daniel A1 Jacques, Mario A1 Fairbrother, JohnYR 1992 T1 Molecular cloning of a determinant coding for fimbrial antigen F1651, a Prs-like fimbrial antigen from porcine septicaemic Escherichia coli JF Microbiology, VO 138 IS 7 SP 1495 OP 1502 DO https://doi.org/10.1099/00221287-138-7-1495 PB Microbiology Society, SN 1465-2080, AB The genetic determinant coding for F1651 fimbriae was cloned from the chromosome of the porcine Escherichia coli wild-type strain 4787 (O115:K−:H51:F165). The fimbrial determinant was further subcloned into the BamHI site of pACYC184 and a restriction map was established. On Southern hybridization, identity between the chromosomally encoded prs-like determinant of strain 4787 and its cloned counterparts was demonstrated. The cloned F1651 fimbriae and those of the wild-type strain possessed a major protein subunit of molecular mass 18.5 kDa. Strains expressing F1651 fimbriae were detected using an F165-specific polyclonal antiserum and caused mannose-resistant haemagglutination and agglutination of Forssman latex beads. Antiserum against the cloned F1651 fimbriae recognized a 18.5 kDa band in the parent strain 4787., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-7-1495