%0 Journal Article %A Harel, Josée %A Forget, Céline %A Saint-Amand, Josée %A Daigle, France %A Dubreuil, Daniel %A Jacques, Mario %A Fairbrother, John %T Molecular cloning of a determinant coding for fimbrial antigen F1651, a Prs-like fimbrial antigen from porcine septicaemic Escherichia coli %D 1992 %J Microbiology, %V 138 %N 7 %P 1495-1502 %@ 1465-2080 %R https://doi.org/10.1099/00221287-138-7-1495 %I Microbiology Society, %X The genetic determinant coding for F1651 fimbriae was cloned from the chromosome of the porcine Escherichia coli wild-type strain 4787 (O115:K−:H51:F165). The fimbrial determinant was further subcloned into the BamHI site of pACYC184 and a restriction map was established. On Southern hybridization, identity between the chromosomally encoded prs-like determinant of strain 4787 and its cloned counterparts was demonstrated. The cloned F1651 fimbriae and those of the wild-type strain possessed a major protein subunit of molecular mass 18.5 kDa. Strains expressing F1651 fimbriae were detected using an F165-specific polyclonal antiserum and caused mannose-resistant haemagglutination and agglutination of Forssman latex beads. Antiserum against the cloned F1651 fimbriae recognized a 18.5 kDa band in the parent strain 4787. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-7-1495