1887

Abstract

The genetic determinant coding for F165 fimbriae was cloned from the chromosome of the porcine wild-type strain 4787 (O115:K:H51:F165). The fimbrial determinant was further subcloned into the HI site of pACYC184 and a restriction map was established. On Southern hybridization, identity between the chromosomally encoded -like determinant of strain 4787 and its cloned counterparts was demonstrated. The cloned F165 fimbriae and those of the wild-type strain possessed a major protein subunit of molecular mass 18.5 kDa. Strains expressing F165 fimbriae were detected using an F165-specific polyclonal antiserum and caused mannose-resistant haemagglutination and agglutination of Forssman latex beads. Antiserum against the cloned F165 fimbriae recognized a 18.5 kDa band in the parent strain 4787.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1495
1992-07-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1495.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1495&mimeType=html&fmt=ahah

References

  1. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., Väisänen-Rhen V., Korhonen T. K., Stuart S., Ørskov F., Ørskov I. 1986; Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infection and Immunity 51 268 276
    [Google Scholar]
  2. Archambaud M., Courcoux P., Labigne-Roussel A. 1988; Detection by molecular hybridization of PAP, AFA, and SFA adherence systems in Escherichia coli strains associated with urinary and enteral infections. Annales de Vlnstitut Pasteur/Microbiologie 139 575 588
    [Google Scholar]
  3. Bakker D., Van Zijderveld G., Van der Veen S., Oudega B., de Graaf F. K. 1990; K88 fimbriae as carriers of heterologous antigenic determinants. Microbiology Pathogenesis 8 343 352
    [Google Scholar]
  4. Båga M., Normark S., Hardy J., O’Hanley P., Lark D., Olsson O., Schoolnik G., Falkow S. 1984; Nucleotide sequence of the PapA pilus subunit of human uropathogenic Escherichia coli. Journal of Bacteriology 157 330 333
    [Google Scholar]
  5. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7 1513 1523
    [Google Scholar]
  6. Broes A., Fairbrother J. M., Jacques J., Larivière S. 1988; Isolation and characterization of a new fimbrial antigen (CS1541) from a porcine enterotoxigenic Escherichia coli O8:KX105 strain. FEMS Microbiology Letters 55 341 348
    [Google Scholar]
  7. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from p15A cryptic miniplasmid. Journal of Bacteriology 134 1141 1156
    [Google Scholar]
  8. Contrepois M., Fairbrother J. M., Kaura Y. K., Girardeau J. P. 1989; Prevalence of CS31A and F165 surface antigens in Escherichia coli isolates from animals in France, Canada, and India. FEMS Microbiology Letters 59 319 324
    [Google Scholar]
  9. Fairbrother J. M., Larivière S., Lallier R. 1986; New fimbrial antigen F165 from Escherichia coli serogroup O115 strains isolated from piglets with diarrhoea. Infection and Immunity 51 10 15
    [Google Scholar]
  10. Fairbrother J. M., Lallier R., Leblanc L., Jacques M., Larivière S. 1988a; Production and purification of Escherichia coli fimbrial antigen F165. FEMS Microbiology Letters 56 247 252
    [Google Scholar]
  11. Fairbrother J. M., Larivière S., Johnson W. M. 1988b; Prevalence of fimbrial antigens and enterotoxins in nonclassical serogroups of Escherichia coli isolated from newborn pigs with diarrhea. American Journal of Veterinary Research 49 1325 1328
    [Google Scholar]
  12. Fairbrother J. M., Broes A., Jacques M., Larivière S. 1989; Pathogenicity of Escherichia coli O115:KV165 isolated from pigs with diarrhea. American Journal of Veterinary Research 50 1029 1036
    [Google Scholar]
  13. Frey J., Bagdasarian M., Feiss D., Franklin C. H., Deshusses J. 1983; Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24 299 308
    [Google Scholar]
  14. Garcia E., Bergmans H. E. N., Van den Bosch J. F., Ørskov I., Van der Zeikst B. A. M., Gaastra W. 1982; Isolation and characterization of dog uropathogenic Escherichia coli strains and their fimbriae. Antonie van Leeuwenhoek 54 149 163
    [Google Scholar]
  15. Hacker J. 1989; Genetic determinants coding for fimbriae and adhesins of extra-intestinal Escherichia coli. Current Topics in Microbiology and Immunology 151 1 28
    [Google Scholar]
  16. Hacker J., Schmidt G., Hughes C., Knapp S., Marget M., Goebel W. 1985; Cloning and characterization of genes involved in production of mannose-resistant, neuramidase susceptible (X) fimbriae from a uropathogenic Escherichia coli. Infection and Immunity 47 434 440
    [Google Scholar]
  17. Harel J., Daigle F., Maiti S., Desautels C., Labigne A., Faibrother J. M. 1991a; Occurence of pap-, sfa-, and afa-related sequences among F165-positive Escherichia coli from diseased animals. FEMS Microbiology Letters 82 177 182
    [Google Scholar]
  18. Harel J., Lapointe H., Fallara A., Lortie L. A., Bigras-Poulin M., Larivière S., Fairbrother J. M. 1991b; Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhoea. Journal of Clinical Microbiology 29 745 752
    [Google Scholar]
  19. Hull R. A., Gill R. E., Hsu P., Minshew H., Falkow S. 1981; Construction and expression of recombinant plasmids encoding type 1 or d-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infection and Immunity 33 933 938
    [Google Scholar]
  20. Källenius G., Möllby R., Svensson S. B., Winberg J., Lundblad A., Svensson S., Cedergren B. 1980; The Pk antigen as receptor for the haemagglutination of pyelonephritogenic Escherichia coli. FEMS Microbiology Letters 7 297 302
    [Google Scholar]
  21. Karr J. F., Nowicki B., Truong L. D., Hull R. A., Hull S. I. 1989; Purified P fimbriae from two cloned gene clusters of a single strain adhere to unique structures in the human kidney. Infection and Immunity 57 3594 3600
    [Google Scholar]
  22. Korhonen T. K., Väisänen-Rhen V., Rhen M., Pere A., Parkkinen F., Finne J. 1984; Escherichia coli fimbriae recognizing sialyl galactosides. Journal of Bacteriology 159 762 766
    [Google Scholar]
  23. Kushner S. R. 1978; An improved method for transformation of Escherichia coli with ColEl-derived plasmids. In Genetic Engineering pp. 17 23 Edited by Boyer H. B., Nicosia S. Amsterdam: Elsevier;
    [Google Scholar]
  24. Labigne-Roussel A., Lark D., Schoolnik G., Falkow S. 1984; Cloning and expression of an afimbrial adhesin (AFA-I) responsible for P blood group-independent, mannose-resistant haemagglutination from a pyelonephritic Escherichia coli strain. Infection and Immunity 46 251 259
    [Google Scholar]
  25. Legendre N., Matsudaira P. 1988; Direct microsequencing from Immobilon-P transfer membrane. Biotechniques 6 154 159
    [Google Scholar]
  26. Lindstedt R., Baker N., Falk P., Hull R., Hull S., Karr J., Leffler H., Svanborg-Edén C., Larson G. 1989; Binding specificities of wild-type and cloned Escherichia coli strains that recognize globo-A. Infection and Immunity 57 3389 3394
    [Google Scholar]
  27. Lindstedt R., Larson G., Falk P., Jodal U., Leffler H., Svanborg C. 1991; The receptor repertoire defines the host range for attaching Escherichia coli that recognize globo-A. Infection and Immunity 59 1086 1092
    [Google Scholar]
  28. Lortie L. A., Dubreuil J. D., Harel J. 1991; Characterization of Escherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea. Journal of Clinical Microbiology 29 656 659
    [Google Scholar]
  29. Low D. A., Braaten B. A., Ling G. V., Johnson D. L., Ruby A. L. 1988; Isolation and comparison of Escherichia coli strains from canine and human patients with urinary tract infections. Infection and Immunity 56 2601 2609
    [Google Scholar]
  30. Lund B., Marlund B. I., Strömberg N., Lindberg F., Karlsson K. A., Normark S. 1988; Uropathogenic E. coli can express serologically identical pili of different receptor binding specificities. Molecular Microbiology 2 255 263
    [Google Scholar]
  31. Maniatis T., Fritsch E. F., Sambrook J. 1983 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Orino K., Naiki M. 1990; Two kinds of P-fimbrial variants of uropathogenic Escherichia coli recognizing Forssman glycosphingolipid. Microbiology and Immunology 34 607 615
    [Google Scholar]
  34. ØRSKOV I., ØRSKOV F. 1985; Escherichia coli in extraintestinal infections. Journal of Hygiene 95 551 575
    [Google Scholar]
  35. ØRSKOV I., ØRSKOV F. 1983; Serology of Escherichia coli fimbriae. Progress in Allergy 33 80 105
    [Google Scholar]
  36. Ott M., Bender L., Blum G., Schmittroth M., Achtman M., Tschape H., Hacker J. 1991; Virulence patterns genetic mapping of extraintestinal Escherichia coli Kl, K5, and K100 isolates: use of pulse-field electrophoresis. Infection and Immunity 59 2664 2672
    [Google Scholar]
  37. Rhen M., Van Die I., Rhen V., Bergmans H. 1985; Comparisons of the nucleotide sequences of the genes encoding the KS71A and F71 fimbrial antigens of uropathogenic Escherichia coli. European Journal of Biochemistry 151 573 577
    [Google Scholar]
  38. Rhen M., Klemm P., Korhonen T. K. 1986; Identification of two new hemagglutinins of Escherichia coli, N-acetyl-d-glucosamine-specific fimbriae and a blood group M-specific agglutinin, by cloning the corresponding genes in Escherichia coli K-12. Journal of Bacteriology 168 1234 1242
    [Google Scholar]
  39. Senior D., Baker N., Cedergren B., Falk P., Larson G., Lindstedt R., Svanborg-Eden C. 1988; Globo-A – a new receptor specificity for attaching Escherichia coli. FEBS Letters 237 123 127
    [Google Scholar]
  40. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98 503 517
    [Google Scholar]
  41. Strömberg N., Marklund B.-I., Lund B., Ilver D., Hamers A., Gaastra W., Karlsson K.-A., Normark N. 1990; Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Galαl-4Gal-containing isoreceptors. EMBO Journal 9 2001 2010
    [Google Scholar]
  42. Strömberg N., Nyholm P.-G., Pascher I., Normark S. 1991; Saccharide orientation at the cell surface affects glycolipid receptor function. Proceedings of the National Academy of Sciences of the United States of America 88 9340 9344
    [Google Scholar]
  43. Svanborg-Edén C., De Man P. 1987; Bacterial virulence in urinary tract infection. Infection and Disease Clinical North American 1 731 750
    [Google Scholar]
  44. Tennent J. M., Hultgren S., Göransson M., Uhlin B. E., Normark S. 1990; Genetics of adhesin expression in Escherichia coli. In Molecular Basis of Bacterial Pathogenesis pp. 80 135 Edited by Iglewski B. H., Clark V. L. San Diego: Academic Press;
    [Google Scholar]
  45. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 78 4350 4353
    [Google Scholar]
  46. Väisänen-Rhen V., Elo J., Väisänen E., Siitonen A., Ørskov I., Ørskov F., Svenson S. B., Mäkelä P. H., Korhonen T. K. 1984; P-fimbriated clones among uropathogenic Escherichia coli strains. Infection and Immunity 43 149 155
    [Google Scholar]
  47. Van Die I., Bergmans H. 1984; Nucleotide sequence of the gene encoding the F72 fimbrial subunit of a uropathogenic Escherichia coli strain. Gene 32 83 90
    [Google Scholar]
  48. Van Die I., Hoekstra W., Bergmans H. 1987; Analysis of the primary structure of P-fimbrillins of uropathogenic Escherichia coli. Microbial Pathogenesis 3 149 154
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-7-1495
Loading
/content/journal/micro/10.1099/00221287-138-7-1495
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error