Auxotrophic mutants were isolated in two strains of Trichosporon cutaneum. Complementing auxotrophs were hybridized by protoplast fusion. Some of the fusants were apparently transient diploids and segregated to give recombinant marker combinations.
De MotR.,
DemeersmanH.,
VerachtertH.1984;
Comparative study of starch degradation and amylase production by nonascomycetous yeast species. Systematic and Applied Microbiology5421432
FiechterA.,
KäppeliO.,
MeussdoerfferF.1987;
Batch and continous culture.
In
The Yeasts
vol.
2
pp.
99129
Edited by
RoseA. H.,
HarrisonJ. S.
London:
Academic Press;
GalzyP.,
SlonimskiP. P.1957;
Chimie biologique -variations physiologiques de la levure au cours de la croissance sur l’acide lactique comme seule source de carbone. Comptes Rendus de l’Académie des Sciences, Paris24524232426
HamlynP. F.,
BradshawR. E.,
MellonF. M.,
SantiagoC. M.,
WilsonJ. M.,
PeberdyJ. F.1981;
Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology3321325
HrmovaM.,
BielyP.,
VrsanskaM.,
PetrakovaE.1984;
Induction of cellulose- and xylan-degrading enzyme complex in the yeast Trichosporon cutaneum. Archives of Microbiology138371376
MessenguyF.,
ColinD.,
ten HaveJ. P.1980;
Regulation of compartmentation of amino acid pools in Saccharomyces cereυisiae and its effects on metabolic control. European Journal of Biochemistry108439447
OchsnerU.,
GlumoffV.,
KälinM.,
FiechterA.,
ReiserJ.1991;
Genetic transformation of auxotrophic mutants of the filamentous yeast Trichosporon cutaneum using homologous and heterologous marker genes. Yeast7513524
PetkovP.,
KostovV.,
BalashevaM.,
BankovI.1984;
The biosynthesis of protein and its amino acid content in strains of Trichosporon cutaneum cultured on nutrient media containing whey. Acta Microbiologica Bulgarica145661