Molecular characterization of two bacteriophages isolated from NCIMB 8303 (Hildenborough) Free

Abstract

A preliminary endonuclease restriction map of a bacteriophage isolated from has been established. HI cleaved whole phage DNA into four fragments while III cut the same DNA into seven fragments. Mapping studies succeeded in linking the four HI fragments into two DNA segments; however, no linkage between the two segments was detected. These data imply that two phages were induced from cultures of and that the two segments represented the DNA from these phages. Support for this hypothesis came from size approximation of restriction enzyme fragments, electron micrographs, and density gradients.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1393
1992-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1393.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1393&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacterio-cins. Bacteriological Reviews 31 230 314
    [Google Scholar]
  3. Garon C. F. 1986; Ultrastructure techniques for microorganisms. In Electron Microscopy of Nucleic Acids pp. 161 181 Edited by Aldrich H. C., Todd W. J. New York: Plenum Press;
    [Google Scholar]
  4. Handley E. J., Adams V., Akagi J. M. 1973; Morphology of bacteriophage-like particles from Desulfovibrio vulgaris. Journal of Bacteriology 115 1205 1207
    [Google Scholar]
  5. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9 2989 2998
    [Google Scholar]
  6. Kamimura K., Araki M. 1989; Isolation and characterization of a bacteriophage lytic for Desulfovibrio salexigens, a salt-requiring sulfate-reducing bacterium. Applied and Environmental Microbiology 55 645 648
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Postgate J. R. 1984 The Sulphate-Reducing Bacteria , 2nd edn.. Cambridge: Cambridge University Press;
    [Google Scholar]
  9. Rapp B. J., Wall J. D. 1987; Genetic transfer in Desulfovibrio desulfuricans. Proceedings of the National Academy of Sciences of the United States of America 8 9128 9130
    [Google Scholar]
  10. Seyedirashti S., Wood C., Akagi J. M. 1991; Induction and partial purification of bacteriophages from Desulfovibrio vulgaris (Hildenborough) and Desulfovibrio desulfuricans ATCC 13541. Journal of General Microbiology 137 1545 1549
    [Google Scholar]
  11. Weigle J. J., Delbruck M. 1951; Mutual exclusion between an infecting phage and a carried phage. Journal of Bacteriology 62 301 318
    [Google Scholar]
  12. Westmoreland B. C., Sztbaksju W., Ris H. 1969; Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage Lambda by electron microscopy. Science 163 1343 1348
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-7-1393
Loading
/content/journal/micro/10.1099/00221287-138-7-1393
Loading

Data & Media loading...

Most cited Most Cited RSS feed