A preliminary endonuclease restriction map of a bacteriophage isolated from has been established. HI cleaved whole phage DNA into four fragments while III cut the same DNA into seven fragments. Mapping studies succeeded in linking the four HI fragments into two DNA segments; however, no linkage between the two segments was detected. These data imply that two phages were induced from cultures of and that the two segments represented the DNA from these phages. Support for this hypothesis came from size approximation of restriction enzyme fragments, electron micrographs, and density gradients.


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