1887

Abstract

The conjugative properties of an indigenous 85 MDa plasmid (designated pCH1) from were studied. To determine if pCH1 was transmissible by conjugation, mating experiments were performed between legionellae that harboured pCH1 and several plasmid-less recipients. Plasmid transfer was monitored by colony hybridization, using a cloned 21.0 kb I restriction fragment from pCH1 as a probe. The results from these experiments showed that pCH1 could be conjugatively transferred into several strains of serogroup 1 but not into strain Bloomington-2 (serogroup 3) or . Southern hybridization experiments in which pCH1 DNA was used as a probe showed that pCH1 does not share homology with other indigenous plasmids. There was no detectable DNA homology between pCH1 and chromosomal DNA. Additional mating experiments revealed that pCH1 was unable to mobilize the chromosome. The conjugative transfer of pCH1 into plasmid-less avirulent or virulent serogroup 1 strains did not alter the intracellular growth characteristics of these strains in U937 cells, a human-monocyte-like cell line, or in the amoeba . These results suggest that pCH1 does not contribute to the ability of to enter or grow within eukaryotic cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1379
1992-07-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1379.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1379&mimeType=html&fmt=ahah

References

  1. Bartowsky E. J., Morelli G., Kamke M., Manning P. 1987; Characterization and restriction analysis of the P sex factor and the cryptic plasmid of Vibrio cholerae strain V58. Plasmid 18 1 7
    [Google Scholar]
  2. Ben-Gurion R., Shafferman A. 1981; Essential virulence determinants of different Yersinia species are carried on a common plasmid. Plasmid 5 183 187
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7 1513 1523
    [Google Scholar]
  4. Bollin C. E., Plouffe J. F., Para M. F., Prior R. B. 1985; Difference in virulence of environmental isolates of Legionella pneumophila. Journal of Clinical Microbiology 21 674 677
    [Google Scholar]
  5. Bouanchaud D. H., Scavizzi M. R., Chabbert Y. A. 1969; Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. Journal of General Microbiology 54 417 425
    [Google Scholar]
  6. Brenton A. M., Jaoua S., Guespin-Michel J. 1985; Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome. Journal of Bacteriology 161 523 528
    [Google Scholar]
  7. Chen G. C. C., Lema M., Brown A. 1984; Plasmid transfer into members of the Legionellaceae. Journal of Infectious Diseases 150 513 516
    [Google Scholar]
  8. Chen G. C. C., Brown A., Lema M. W. 1986; Restriction endonuclease activities in the legionellae. Canadian Journal of Microbiology 32 591 593
    [Google Scholar]
  9. Deich R. A., Green B. A. 1987; Mobilization of Haemophilus influenzae chromosomal markers by an Escherichia coli F′ factor. Journal of Bacteriology 169 1905 1910
    [Google Scholar]
  10. Dreyfus L. A. 1987; Virulence associated ingestion of Legionella pneumophila by HeLa cells. Microbial Pathogenesis 3 45 52
    [Google Scholar]
  11. Dreyfus L. A., Iglewski B. H. 1985; Conjugation-mediated genetic exchange in Legionella pneumophila. Journal of Bacteriology 161 80 84
    [Google Scholar]
  12. Fields B. S., Barbaree J. M., Shotts E. B., Feeley J. C., Morrill W., Sanden G. S., Dykstra M. J. 1986; Comparison of the guinea pig and protozoan models for determining virulence of Legionella species. Infection and Immunity 53 553 559
    [Google Scholar]
  13. Gulig P. A. 1990; Virulence plasmids of Salmonella typhimurium and other salmonellae. Microbial Pathogenesis 8 3 11
    [Google Scholar]
  14. Holden E. P., Winkler H. H., Wood D. O., Leinbach E. D. 1984; Intracellular growth of Legionella pneumophila within Acanthamoeba castellanii Neff. Infection and Immunity 45 18 24
    [Google Scholar]
  15. Horwitz M. A., Silverstein S. C. 1980; Legionnaires disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. Journal of Clinical Investigation 66 441 450
    [Google Scholar]
  16. Ichige A., Matsutani S., Oishi K., Mizushima S. 1989; Establishment of gene transfer systems for and construction of the genetic map of a marine Vibrio strain. Journal of Bacteriology 171 1825 1834
    [Google Scholar]
  17. Johnson S. R., Schalla W. O. 1982; Plasmids of serogroup 1 strains of Legionella pneumophila. Current Microbiology 7 143 146
    [Google Scholar]
  18. Kado C. I., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145 1365 1373
    [Google Scholar]
  19. King C. H., Fields B. S., Shotts E. B., White E. H. 1991; Effects of cytochalasin D and methylamine on intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells. Infection and Immunity 59 758 763
    [Google Scholar]
  20. Knudson G. B., Mikesell P. 1980; A plasmid in Legionella pneumophila. Infection and Immunity 29 1092 1095
    [Google Scholar]
  21. Maher W. E., Plouffe J. F., Para M. F. 1983; Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1. Journal of Clinical Microbiology 18 1422 1423
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Handbook Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Marra A., Shuman H. A. 1989; Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings. Journal of Bacteriology 171 2238 2240
    [Google Scholar]
  24. Maurelli A. T., Sansonetti P. J. 1988; Genetic determinants of Shigella pathogenicity. Annual Review of Microbiology 42 127 150
    [Google Scholar]
  25. Mikesell P., Ezzell J. W., Knudson G. B. 1981; Isolation of plasmids in Legionella pneumophila and Legionella-like organisms. Infection and Immunity 31 1270 1272
    [Google Scholar]
  26. Mintz C. S., Schuman H. A. 1988; Genetics of Legionella pneumophila. Microbiological Sciences 5 292 295
    [Google Scholar]
  27. Newsome A. L., Baker R. L., Miller R. D., Arnold R. R. 1985; Interactions between Naegleria fowleri and Legionella pneumophila. Infection and Immunity 50 449 452
    [Google Scholar]
  28. Pearlman D., Jiwa A. H., Engelberg N. C., Eisenstein B. I. 1988; Growth of Legionella pneumophila in a human macrophagelike (U937) line. Microbial Pathogenesis 5 87 95
    [Google Scholar]
  29. Pischl D. L., Farrand S. K. 1983; Transposon-facilitated chromosome mobilization in Agrobacterium tumefaciens. Journal of Bacteriology 153 1451 1460
    [Google Scholar]
  30. Portnoy D. L., Mosely S. L., Falkow S. 1981; Characterization of plasmids and plasmid associated determinants of Yersinia enterocolitica pathogenesis. Infection and Immunity 21 75 78
    [Google Scholar]
  31. Portnoy D. A., Wolf-Watz H., Bolin I., Breeder A. B., Falkow S. 1984; Characterization of common virulence plasmids in Yersinia species and their role in the expression of outer membrane proteins. Infection and Immunity 43 108 114
    [Google Scholar]
  32. Riva S., Fietta A., Berti M., Silvestri G., Romero E. 1973; Relationships between curing of the F episome by rifampin and by acridine orange in Escherichia coli. Antimicrobial Agents and Chemotherapy 3 456 462
    [Google Scholar]
  33. Rowbotham T. J. 1986; Current views on the relationships between amoebae, legionellae and man. Israel Journal of Medical Sciences 22 678 689
    [Google Scholar]
  34. Silhavy T. J., Berman M. L., Enquist L. W. 1984 Experiments with Gene Fusions Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Trevors J. T., Oddie K. M., Belliveau B. H. 1985; Metal resistance in bacteria. FEMS Microbiology Reυiews 32 39 54
    [Google Scholar]
  36. Tully M. 1991; A plasmid from a virulent strain of Legionella pneumophila is conjugative and confers resistance to ultraviolet light. FEMS Microbiology Letters 90 43 48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-7-1379
Loading
/content/journal/micro/10.1099/00221287-138-7-1379
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error