1887

Abstract

Strains of the basidiomycete fungi from the USSR and from the eastern USA were interfertile, yielding viable, variable, meiotic progeny. However, interactions between paired homokaryotic mycelia of these species were unstable, yielding a uniform mycelial mat on the side, but a macroscopically heterogeneous mycelium on the side. Hyphal tips excised from the interactions gave rise to colonies identifiable as one or other of the progenitor strains or two non-progenitor types (C-types and H-types). C-types were all morphologically similar to one another and resembled the progenitor except in being inaccessible to nuclear migration and in exhibiting a light-induced phenotypic switch. They could be isolated from both sides of interactions and were somatically compatible with one another. H-types were only isolated from the side of interactions and had a range of morphologies, generally similar to the progenitor. Pairings between H-types, and between H-types and C-types sometimes resulted in pigmented interaction zones due to somatic incompatibility. Single, dual and minisatellite DNA probes were used to investigate restriction fragment length polymorphisms in progenitor and derivative strains. Nuclear, but not mitochondrial DNA from both species was detected in all non-progenitor strains obtained from beyond the interaction interface. However, whereas -specific nuclear sequences were consistently present, the distribution of -specific sequences varied, and was partly correlated with possession of H-type morphologies.

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1992-06-01
2021-04-22
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References

  1. Ainsworth A. M. 1987; Occurrence and interactions of outcrossing and non-outcrossing populations in Stereum, Phanerochaete and Coniophora . Evolutionary Biology of the Fungi285–299 Rayner A. D. M., Brasier C. M., Moore D. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Ainsworth A. M., Rayner A. D. M. 1989; Hyphal and mycelial responses associated with genetic exchange within and between species of the basidiomycete genus Stereum . Journal of General Microbiology 135:1643–1659
    [Google Scholar]
  3. Ainsworth A. M., Rayner A. D. M, Broxholme S. J., Beeching J. R. 1990a; Occurrence of unilateral genetic transfer and genomic replacement between strains of Stereum hirsutum from non-outcrossing and outcrossing populations. New Phytologist 115:119–128
    [Google Scholar]
  4. Ainsworth A. M., Rayner A. D. M, Broxholme S. J., Beeching J. R., Pryke J. Α., Scard P. T., Berriman J., Powell Κ. Α., Floyd A. J., Branch S. K. 1990; Production and properties of the sesquiterpene, ( + )-torreyol, in degenerative mycelial interactions between strains of Stereum . Mycological Research 94:799–809
    [Google Scholar]
  5. Beeching J. R., Ainsworth A. M., Broxholme S. J., Pryke J. A., Rayner A. D. M. 1989; Investigation of genetic transfer between strains of the basidiomycete, Stereum hirsutum, using molecular and morphological criteria. New Phytologist 113:505–512
    [Google Scholar]
  6. Brocas H., Georges M., Cristophe D., Monsieur R., Lequarre A. S., Vassart G. 1987; Une famille de minisatellites hypervariables détectée au moyen d’ une séquence dérivée du phage Ml 3. Comptes Rendus de l’ Académie des Sciences Serie III 304:67–69
    [Google Scholar]
  7. Butler G.M. 1984; Colony ontogeny in the basidiomycetes. The Ecology and Physiology of the Fungal Mycelium53–71 Jennings D. H., Rayner A. D. M. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Casselton L. A., Economou A. 1985; Dikaryon formation. Developmental Biology of Higher Fungi213–229 Moore D., Casselton L. A., Wood D. A., Frankland J. C. Cambridge: Cambridge University Press;
    [Google Scholar]
  9. Coates D., Rayner A. D. M. 1985a; Heterokaryon-homokaryon interactions in Stereum hirsutum . Transactions of the British Mycological Society 84:637–645
    [Google Scholar]
  10. Coates D., Rayner A. D. M. 1985e; Genetic control and variation in expression of the ‘bow-tie’ interaction between homokaryons of Stereum hirsutum . Transactions of the British Mycological Society 84:191–205
    [Google Scholar]
  11. Coates D., Rayner A. D. M. 1985c; Evidence for a cytoplasmic-ally transmissible factor affecting recognition and somato-sexual differentiation in the basidiomycete Stereum hirsutum . Journal of General Microbiology 131:207–219
    [Google Scholar]
  12. Coates D., Rayner A. D. M, Todd N. K. 1981; Mating behaviour, mycelial antagonism and the establishment of individuals in Stereum hirsutum . Transactions of the British Mycological Society 76:41–51
    [Google Scholar]
  13. Devor E. J., Ivanovich A. K., Hickok J. M., Todd R. D. 1988; A rapid method for confirming cell line identity : DNA ‘fingerprinting’ with a minisatellite probe from Ml3 bacteriophage. Bio-Techniques 6:200–202
    [Google Scholar]
  14. Ellingboe A. H. 1964; Somatic recombination in dikaryon Κ of Schizophyllum commune . Genetics 49:247–251
    [Google Scholar]
  15. Feinberg A., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  16. Frankel C., Ellingboe A. H. 1977; Sexual incompatibility factors and somatic recombination in Schizophyllum commune . Genetics 85:427–437
    [Google Scholar]
  17. Hintz W. Ε. Α., Anderson J. H., Horgen P. A. 1988; Nuclear migration and mitochondrial inheritance in the mushroom Agaricus bitorquis . Genetics 119:35–41
    [Google Scholar]
  18. Hooley P., Fyfe A. M., Evola Maltese E., Shaw O. S. 1982; Duplication cycle in nuclei of germinating zoospores of Phytophthora drechsleri as revealed by DAPI staining. Transactions of the British Mycological Society 79:563–566
    [Google Scholar]
  19. Hotchkiss R. D., Gabor M. H. 1985; Protoplast fusion in Bacillus and its consequences. The Molecular Biology of the Bacilli II:109–149 Dubnau D. A. New York: Academic Press;
    [Google Scholar]
  20. Marmeisse R. 1991; Parasexual analysis in the basidiomycete mushroom Agrocybe aegerita common-B and common AB crosses. Mycological Research 95:465–468
    [Google Scholar]
  21. May G. 1988; Somatic incompatibility and individualism in the coprophilous basidiomycete Coprinus cinereus . Transactions of the British Mycological Society 91:443–451
    [Google Scholar]
  22. May G., Taylor J. W. 1988; Patterns of mating and mitochondrial DNA inheritance in the agaric basidiomycete Coprinus cinereus . Genetics 118:213–220
    [Google Scholar]
  23. Monk M., Surani A. 1990 Genomic Imprinting. Cambridge: Company of Biologists;
    [Google Scholar]
  24. Rayner A. D. M. 1991; The challenge of the individualistic mycelium. Mycologia 83:48–71
    [Google Scholar]
  25. Rayner A. D. M, Coates D., Ainsworth A. M., Adams T. J. H, Williams E. N. D, Todd N. K. 1984; The biological consequences of the individualistic mycelium. The Ecology and Physiology of the Fungal Mycelium509–540 Jennings D. H., Rayner A. D. M. Cambridge: Cambridge University Press;
    [Google Scholar]
  26. Rayner A. D. M, Ross I. K. 1991; Sexual politics in the cell. New Scientist 129:30–33
    [Google Scholar]
  27. Rayner R. W. 1970 A Mycological Colour Chart. Kew, Surrey: C.M.I, and British Mycological Society;
    [Google Scholar]
  28. Sokal R. R., Rohlf F. J. 1981 Biometry, 2nd edn. San Francisco: W. H. Freeman;
    [Google Scholar]
  29. Stasz T. E., Harman G. E., Gullino M. L. 1989; Limited vegetative incompatibility following intra- and interspecific protoplast fusion in Trichoderma . Experimental Mycology 13:364–371
    [Google Scholar]
  30. Westneat D. F., Noon W. Α., Reeve H. K., Aquadroa C. F. 1988; Improved hybridization conditions for DNA ‘fingerprints’ probed with M13. Nucleic Acids Research 16:4161
    [Google Scholar]
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