1887

Abstract

A plasmid has been isolated for the first time from moderately halophilic eubacteria. and were found to harbour an 11·5 kbp plasmid (pMH1). The plasmid was isolated and characterized after transformation into JM101 cells. A restriction map was constructed, and unique restriction sites for RI, RV and I were detected. The occurrence of such a plasmid in the original halophilic strains was confirmed by Southern hybridization. The plasmid carries genetic determinants that mediate resistance to kanamycin, tetracycline, and neomycin. This property, together with its relatively small size, its stability in cells, and the presence of unique restriction sites, makes pMH1 a good candidate for the development of a cloning vector for moderate halophiles.

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1992-06-01
2022-01-28
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References

  1. AusuBEL F. M., Brent K., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1989 Current Protocols in Molecular Biology. Greene Publishing Associates, NY: John Wiley and Sons;
    [Google Scholar]
  2. Birnboim H. C. 1983; A rapid alkaline extraction method for the isolation of plasmid DNA. Methods in Enzymology 100:243–255
    [Google Scholar]
  3. Franzmann P. D., Wehmeyer U., Stackebrandt E. 1988; Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . Systematic and Applied Microbiology 11:16–19
    [Google Scholar]
  4. Garcia M. T., Ventosa A., Ruiz-Berraquero F., Kokur M. 1987; Taxonomie study and amended description of Vibrio cost kola . International Journal of Systematic Bacteriology 37:251–256
    [Google Scholar]
  5. Gutierrez M. C, Ventosa A., Nieto J. J., Ruiz-Berraquero F. 1986; Occurrence of megaplasmids in halobacteria. Journal of Applied Bacteriology 61:67–71
    [Google Scholar]
  6. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  7. Kado C. I., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  8. Kamekura M. 1986; Production and function of enzymes of eubacterial halophiles. FEMS Microbiology Reviews 39:145–150
    [Google Scholar]
  9. Kushner D. J. 1978; Life in high salt and solute concentrations: halophilic bacteria. Microbial Life in Extreme Environments317–368 Kushner D. J. London: Academic Press;
    [Google Scholar]
  10. Kushner D. J., Kamekura M. 1988; Physiology of halophilic eubacteria. Halophilic Bacteria109–140 Rodriguez-Valera F. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  11. Magee A. M., Quinn J. P. 1991; Antibiotic resistance in the bacteria of a remote upland river catchment. Letters in Applied Microbiology 13:145–149
    [Google Scholar]
  12. Maniatis T., Fritsch E., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Marquez M. C, Ventosa A., Ruiz-Berraquero F. 1987; A taxonomie study of heterotrophic halophilic and non-halophilic bacteria from a solar saltern. Journal of General Microbiology 133:45–56
    [Google Scholar]
  14. Morelle G. 1989; A plasmid extraction procedure on a miniprep scale. BRL Focus 11:7–8
    [Google Scholar]
  15. Nieto J. J., Fernandez-Castillo R., Marquez M. C, Ventosa A., Ruiz-Berraquero F. 1989; A survey of metal tolerance in moderately halophilic eubacteria. Applied and Environmental Microbiology 55:2385–2390
    [Google Scholar]
  16. Old R. W., Primrose S. B. 1989 Principles of Gene Manipulation. An Introduction to Genetic Engineering, 4th edn. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  17. Onishi H., Mori T., Takeuchi S., Tani K., Kobayashi T., Kamekura M. 1983; Halophilic nuclease of a moderately halophilic Bacillus sp: production, purification, and characterization. Applied and Environmental Microbiology 45:24–30
    [Google Scholar]
  18. Quesada E., Ventosa A. Rodriguez-Valera F., Ramos-Cormenzana A. 1982; Types and properties of some bacteria isolated from hypersaline soils. Journal of Applied Bacteriology 53:155–161
    [Google Scholar]
  19. Rodriguez-Valera F. 1986; The ecology and taxonomy of aerobic chemoorganotrophic halophilic eubacteria. FEMS Microbiology Reviews 39:17–22
    [Google Scholar]
  20. Rodriguez-Valera F., Ventosa A., Juez G., Imhoff J. 1985; Variation of environmental features and microbial populations with salt concentrations in a multipond saltem. Microbial Ecology 11:107–115
    [Google Scholar]
  21. Smith F. B. 1938; An investigation on a taint in rib bones of bacon. The determination of halophilic vibrios (n. spp.). Proceedings of the Royal Society of Queensland 49:29–53
    [Google Scholar]
  22. Southern N. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  23. Vargas C, Martinez L. J., Megias M., Quinto C. 1990; Identification and cloning of nodulation genes and host specifity determinants of the broad host-range Rhizobium leguminosarum biovar phaseoli strain CIAT899. Molecular Microbiology 4:1899–1910
    [Google Scholar]
  24. Ventosa A. 1988; Taxonomy of moderately halophilic heterotrophic eubacteria. Halophilic Bacteria71–84 Rodriguez-Valera F. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  25. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. Journal of General Microbiology 128:1959–1968
    [Google Scholar]
  26. Vreeland R. H. 1984; Genus Halomonas . Bergey’ s Manual of Systematic Bacteriology 1:340–343 Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  27. Yanisch-Perron C, Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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