A bromoperoxidase gene (bpoT), recently cloned from Streptomyces aureofaciens Tü24, was used as a probe in Southern blot hybridization of total DNA from S. aureofaciens ATCC 10762. A single SstI fragment of 5·4 kb was detected, which was cloned via an enriched gene library into Escherichia coli. The functional bromoperoxidase gene was located on a 2.1 kb BamHI-HindIII fragment by subcloning into S. lividans TK64, using the multicopy plasmid pIJ486. The enzyme was overproduced in S. lividans TK64 (up to 30000 times compared to S. aureofaciens ATCC 10762) and showed the same electrophoretic and immunological properties as the bromoperoxidase BPO-A2 purified from S. aureofaciens ATCC 10762. DNA sequence analysis revealed an open reading frame encoding a predicted polypeptide with the same Mr and N-terminal amino acid sequence as the purified subunit of BPO-A2.
AnsorgeW.,
SproatB.,
StegemannI.,
SchwagerC.,
ZenkeM.1987; Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Research 15:4593–4602
BernanV.,
FilpulaD.,
HerberW.,
BibbM. J.,
KatzE.1985; The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product. Gene 37:101–110
BibbM. J.,
CohenS. N.1982; Gene expression in Streptomyces: construction and application of promotor-probe plasmid vectors in Streptomyces lividans
. Molecular and General Genetics 187:265–271
BibbM. J.,
WardJ. H.,
CohenS. N.1985; Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes endogenous to. Streptomyces. Molecular and General Genetics 199:26–39
De BoerE.,
TrompM. G. M,
PlatH.,
KrennB. E.,
WeverR.1986; Vanadium(V) as an essential element for haloperoxidase activity in marine brown algae : purification and characterization of a vanadium(V)-containing bromoperoxidase from. Laminaria sac-charina. Biochimica et Biophysica Acta 872:104–115
GribskovM.,
DeverbauxJ.,
BurgesJ.1984; The codon preference plot : graphic analysis of protein coding sequences and prediction of gene expression. Nucleic Acids Research 12:539–547
HaagT.,
LingensF.,
van PeeK.-H.1991; A metal ion and cofactor-independent enzymatic redox reaction: halogenation by bacterial nonheme haloperoxidases. Angewandte Chemie International Edition in English 30:1487–1488
HopwoodD. A.,
KieserT.,
WrightH. M.,
BibbM. J.1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. Journal of General Microbiology 129:2257–2269
HopwoodD. A.,
BibbM. J.,
ChaterK. F.,
KieserT.,
BrutonC. J.,
KieserH. M.,
LydiateD. J.,
SmithC. P.,
WardJ. M.,
SchrempfH.1985Genetic Manipulation of Streptomyces - a Laboratory Manual. Norwich: John Innes Foundation;
LooT. L.,
BurgerJ. W.,
AdamsonR. H.1964; Bromination of phthalein dyes by the uterus of the dogfish,. Squalus acanthias. Proceedings of the Society of Experimental Biology and Medicine 114:60–63
MizusawaS.,
NishimuraS.,
SeelaF.1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research. 14:1319–1324
OlsenR. L.,
LittleC.1984; Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood. Biochemical Journal 209:781–787
van PEEK.-H.1988; Molecular cloning and high-level expression of a bromoperoxidase gene from Streptomyces aureofaciens Tu24. Journal of Bacteriology 170:5890–5894
van PeeK.-H.,
LingensF.1985; Purification and molecular and catalytic properties of bromoperoxidase from. Streptomyces phaeo-chromogenes. Journal of General Microbiology 131:1911–1916
van PeeK.-H.,
SuryG.,
LingensF.1987; Purification and properties of a nonheme bromoperoxidase from Streptomyces
. aureofaciens. Biological Chemistry Hoppe-Seyler 368:1225–1232
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-termination inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
SchaggerH.,
von JagowG.1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166:368–379
SoberH.,
HaagT.,
PfeiferO.,
SchomburgD.,
LingensF.,
van PeeK.-H.1991; Crystallization and preliminary x-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762. Journal of Molecular Biology 111:35–37
TohyamaH.,
OkamiY.,
UmezawaH.1987; Nucleotide sequence of the streptomycin-phosphotransferase and amidinotransferase genes from. Streptomyces griseus. Nucleic Acids Research 15:1819–1833
WardJ. M.,
JanssenG. R.,
KieserT.,
BibbM. J.,
ButtnerM. J.,
BibbM. J.1986; Construction and characterization of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Molecular and General Genetics 203:468–478
WengM.,
PfeiferO.,
KraussS.,
LingensF.,
van PeeK.-H.1991; Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762. Journal of General Microbiology 137:2539–2546
WiesnerW.van PeeK.-H., LingensF.1988; Purification and characterization of a novel bacterial non-heme chloroperoxidase from. Pseudomonas pyrrocinia. Journal of Biological Chemistry 263:13725–13732