Molecular cloning and sequencing of a non-haem bromoperoxidase gene from ATCC 10762 Free

Abstract

A bromoperoxidase gene (), recently cloned from Tü24, was used as a probe in Southern blot hybridization of total DNA from ATCC 10762. A single I fragment of 5·4 kb was detected, which was cloned via an enriched gene library into . The functional bromoperoxidase gene was located on a 2.1 kb HI-dIII fragment by subcloning into TK64, using the multicopy plasmid pIJ486. The enzyme was overproduced in TK64 (up to 30000 times compared to ATCC 10762) and showed the same electrophoretic and immunological properties as the bromoperoxidase BPO-A2 purified from ATCC 10762. DNA sequence analysis revealed an open reading frame encoding a predicted polypeptide with the same and N-terminal amino acid sequence as the purified subunit of BPO-A2.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-6-1123
1992-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/6/mic-138-6-1123.html?itemId=/content/journal/micro/10.1099/00221287-138-6-1123&mimeType=html&fmt=ahah

References

  1. Akhrem A. A., Drozhdenyuk A. P. 1989; Calcium tartrate gel. Analytical Biochemistry 179:86–89
    [Google Scholar]
  2. Ansorge W., Sproat B., Stegemann I., Schwager C., Zenke M. 1987; Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Research 15:4593–4602
    [Google Scholar]
  3. Bernan V., Filpula D., Herber W., Bibb M. J., Katz E. 1985; The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product. Gene 37:101–110
    [Google Scholar]
  4. Bibb M. J., Cohen S. N. 1982; Gene expression in Streptomyces: construction and application of promotor-probe plasmid vectors in Streptomyces lividans . Molecular and General Genetics 187:265–271
    [Google Scholar]
  5. Bibb M. J., Ward J. H., Cohen S. N. 1985; Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes endogenous to. Streptomyces. Molecular and General Genetics 199:26–39
    [Google Scholar]
  6. De Boer E., Tromp M. G. M, Plat H., Krenn B. E., Wever R. 1986; Vanadium(V) as an essential element for haloperoxidase activity in marine brown algae : purification and characterization of a vanadium(V)-containing bromoperoxidase from. Laminaria sac-charina. Biochimica et Biophysica Acta 872:104–115
    [Google Scholar]
  7. Gibson T. 1984 Studies on the Epstein-Bar virus genome. PhD thesis, University of Cambridge, UK
    [Google Scholar]
  8. Gribskov M., Deverbaux J., Burges J. 1984; The codon preference plot : graphic analysis of protein coding sequences and prediction of gene expression. Nucleic Acids Research 12:539–547
    [Google Scholar]
  9. Haag T., Lingens F., van Pee K.-H. 1991; A metal ion and cofactor-independent enzymatic redox reaction: halogenation by bacterial nonheme haloperoxidases. Angewandte Chemie International Edition in English 30:1487–1488
    [Google Scholar]
  10. Hewson W. D., Hager L. P. 1980; Bromoperoxidase and halogenated lipids in marine algae. Journal of Phycology 16:340–345
    [Google Scholar]
  11. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. Journal of General Microbiology 129:2257–2269
    [Google Scholar]
  12. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces - a Laboratory Manual. Norwich: John Innes Foundation;
    [Google Scholar]
  13. Kieser T. 1984; Factors affecting the isolation of ccc DNA from Streptomyces lividans and Escherichia coli . Plasmid 12:19–36
    [Google Scholar]
  14. Loo T. L., Burger J. W., Adamson R. H. 1964; Bromination of phthalein dyes by the uterus of the dogfish,. Squalus acanthias. Proceedings of the Society of Experimental Biology and Medicine 114:60–63
    [Google Scholar]
  15. Mandel M., Higa A. 1970; Calcium dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Maurer H. R. 1964 Disk-Elektrophorese : Theorie und Praxis der diskontinuierlichen Polyacrylamidgelelektrophorese. Berlin: Walter de Gruyter;
    [Google Scholar]
  18. Mizusawa S., Nishimura S., Seela F. 1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research. 14:1319–1324
    [Google Scholar]
  19. Neidleman S. L., Geigert I. 1986 Biohalogenation : Principles, Basic Roles and Applications. Chichester: Ellis Horwood/John Wiley;
    [Google Scholar]
  20. Olsen R. L., Little C. 1984; Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood. Biochemical Journal 209:781–787
    [Google Scholar]
  21. van PEE K.-H. 1988; Molecular cloning and high-level expression of a bromoperoxidase gene from Streptomyces aureofaciens Tu24. Journal of Bacteriology 170:5890–5894
    [Google Scholar]
  22. van Pee K.-H., Lingens F. 1985; Purification and molecular and catalytic properties of bromoperoxidase from. Streptomyces phaeo- chromogenes. Journal of General Microbiology 131:1911–1916
    [Google Scholar]
  23. van Pee K.-H., Sury G., Lingens F. 1987; Purification and properties of a nonheme bromoperoxidase from Streptomyces . aureofaciens. Biological Chemistry Hoppe-Seyler 368:1225–1232
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-termination inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  25. Schagger H., von Jagow G. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166:368–379
    [Google Scholar]
  26. Sober H., Haag T., Pfeifer O., Schomburg D., Lingens F., van Pee K.-H. 1991; Crystallization and preliminary x-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762. Journal of Molecular Biology 111:35–37
    [Google Scholar]
  27. Tautz D., Renz M. 1983; An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
  28. Tohyama H., Okami Y., Umezawa H. 1987; Nucleotide sequence of the streptomycin-phosphotransferase and amidinotransferase genes from. Streptomyces griseus. Nucleic Acids Research 15:1819–1833
    [Google Scholar]
  29. Ward J. M., Janssen G. R., Kieser T., Bibb M. J., Buttner M. J., Bibb M. J. 1986; Construction and characterization of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Molecular and General Genetics 203:468–478
    [Google Scholar]
  30. Weng M., Pfeifer O., Krauss S., Lingens F., van Pee K.-H. 1991; Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762. Journal of General Microbiology 137:2539–2546
    [Google Scholar]
  31. Wiesner W. van Pee K.-H., Lingens F. 1988; Purification and characterization of a novel bacterial non-heme chloroperoxidase from. Pseudomonas pyrrocinia. Journal of Biological Chemistry 263:13725–13732
    [Google Scholar]
  32. Yanisch-Perron C, Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-6-1123
Loading
/content/journal/micro/10.1099/00221287-138-6-1123
Loading

Data & Media loading...

Most cited Most Cited RSS feed