A bromoperoxidase gene (), recently cloned from Tü24, was used as a probe in Southern blot hybridization of total DNA from ATCC 10762. A single I fragment of 5·4 kb was detected, which was cloned via an enriched gene library into . The functional bromoperoxidase gene was located on a 2.1 kb HI-dIII fragment by subcloning into TK64, using the multicopy plasmid pIJ486. The enzyme was overproduced in TK64 (up to 30000 times compared to ATCC 10762) and showed the same electrophoretic and immunological properties as the bromoperoxidase BPO-A2 purified from ATCC 10762. DNA sequence analysis revealed an open reading frame encoding a predicted polypeptide with the same and N-terminal amino acid sequence as the purified subunit of BPO-A2.


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