The precise location of the rhaT gene, encoding rhamnose permease, has been established between sodA and rhaC at 3605–3607 kb of Kohara's physical map, which corresponds to 88·4 min on the Escherichia coli chromosomal map. The dependence of the activity of the rhaT product on the function of rhaC, the rhamnose operon regulatory gene, was established by measuring rhamnose transport in wild-type and rhaC-deficient strains. The sequence of the sodA−rhaC interval displayed a single ORF corresponding to rhaT, which is transcribed counterclockwise on the E. coli chromosome. The ORF was shown to be preceded by a ribosome binding consensus sequence and a catabolite repression protein consensus sequence. The derived amino acid sequence displayed very low homology with any other permease and was clearly dissimilar to the homologous group formed by the xylose, arabinose, galactose and several glucose transporters. Analysis of the rhaT primary sequence identified potential membrane-spanning regions, possibly defining a protein structure model different from the one corresponding to the above-mentioned homologous group.
BadiaJ.,
BaldomaL.,
AguilarJ.,
BoronatA.1989; Identification oi the rha A, rhaB and rhaD gene products from Escherichia coli K-12. FEMS Microbiology Letters 65:253–258
BedouelleH.,
BassfordP. J.Jr,
FowlerA. W.,
ZabinI.,
BeckwithJ.,
HofnungM.1980; Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli
. Nature, London 285:78–81
BoronatA.,
AguilarJ.1979; Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme. Journal of Bacteriology 140:320–326
BruijnF. J.,
LupskiJ. R.1984; The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids. Gene 27:131–149
Collado-VidesJ.,
MagasanikB.,
GrallaJ. D.1991; Control site location and transcriptional regulation in Escherichia coli
. Microbiological Reviews 55:371–394
DavisE. O.,
HendersonP. J. F.
1987; The cloning and DNA sequence of the gene xylE for xylose-proton symport in E. coli K-12. Journal of Biological Chemistry 262:13928–13932
GarnierJ.,
OsguthorpeD. J.,
RobsonB.1978; Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
HackingA. J.,
LinE. C. C.
1976; Disruption of a fucose pathway as a consequence of genetic adaptation to propanediol as a carbon source in Escherichia coli
. Journal of Bacteriology 126:1166–1172
HendersonP. J. F,
MaidenM. C. J.1990; Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes. Philosophical Transactions of the Royal Society of London 326:391–10
IkemuraT.1981; Correlation between the abundance of E. coli transfer RNA and the occurrence of the respective codons in its proteins genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. Journal of Molecular Biology 151:389–09
KoharaY.,
AkiyamaK.,
IsonoK.1987; The physical map of the whole E. coli chromosome : application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
MaidenM. C. J,
Jones-MortimerM. C.,
HendersonP. J. F.1988; The cloning, DNA sequence and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K-12. Journal of Biological Chemistry 263:8003–8010
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
ScriptureJ. B.,
HoggR. W.1983; The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein. Journal of Biological Chemistry 258:10853–10855
TobinJ. F.,
SchleifR. F.1987; Positive regulation of the Escherichia coli L-rhamnose Operon is mediated by the products of tandemly repeated regulatory genes. Journal of Molecular Biology 196:789–799