1887

Abstract

SUMMARY: rRNA sequences were used as targets for synthetic oligonucleotides labelled with the fluorescent dye tetramethylrhodamine isothiocyanate (Tritc) for hybridizations to detect micro-organisms directly in soils that have different contents of soil minerals and organic material. Introduced cells were directly fixed in soils and applied to slides after separation of large soil minerals only. Remaining soil minerals (clay minerals) and organic material (up to 8%) did not significantly interfere with signal expression after hybridization. Background signals were mainly caused by autofluorescence of organic material. Non-specific binding of labelled oligonucleotides to soil particles was not observed. detection of introduced cells of in a sandy loam spiked with a mixture of selected soil micro-organisms was possible after hybridization with a specific probe. Analysis of natural bacterial populations in soil, however, was not possible by hybridization without activation of these micro-organisms by adding nutrients. Growing cells, e.g. hyphae growing in amended soil, were easily detected.

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/content/journal/micro/10.1099/00221287-138-5-879
1992-05-01
2019-09-18
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-5-879
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