1887

Abstract

Summary: Phospholipase activities releasing fatty acyl moieties from phosphatidylcholine and phosphatidylethanolamine and lysophospholipase activity releasing fatty acid from -phosphatidylcholine were detected in both and Fatty acyl groups were released from both the 1- and 2-positions of phosphatidylcholine. Generally, phospholipase activities of were cryptic while phospholipase activities of were located on the bacterial surface. However, intact did not release fatty acids from phospholipids faster than Neither Mycobacterium secreted acyl-hydrolysing phospholipase activity. All phospholipase activities were stimulated by including phospholipids in growth media: generally, cell extracts contained 6- to 15-fold higher specific activities than extracts from mycobacteria grown in media without added phospholipid. However, not all phospholipase activities were stimulated to the same degree in any given set of conditions, suggesting the existence of more than one phospholipase gene in each Mycobacterium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-4-825
1992-04-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/4/mic-138-4-825.html?itemId=/content/journal/micro/10.1099/00221287-138-4-825&mimeType=html&fmt=ahah

References

  1. Chadwick M. V. 1982; Mycobacteria. Institute for Medical Laboratory Sciences Monographs Bristol: P. S. G. Wright;
    [Google Scholar]
  2. Daum G., Paltauf F. 1990; Lipid transport in microorganisms. Experientia 46:586–592
    [Google Scholar]
  3. Dennis E. A. 1983; Phospholipases. In Lipid Enzymology: The Enzymes vol. 16 pp. 307–353 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
  4. Draper P. 1982; The anatomy of mycobacteria. In The Biology of the Mycobacteria vol. 1 pp. 9–52 Edited by Ratledge C., Stanford J. London: Academic Press;
    [Google Scholar]
  5. Finnerty W. R. 1989; Microbial lipid metabolism. In Microbial Lipids vol. 2 pp. 525–566 Edited by Ratledge C., Wilkinson S. G. London: Academic Press;
    [Google Scholar]
  6. Kashiwabara Y., Nakagawa H., Matsuki G. 1980; Phospholipid deacylating activities in murine leprosy bacilli. Journal of Biochemistry 88:1861–1868
    [Google Scholar]
  7. Kondo E., Suzuki K., Kanai K., Yasuda T. 1985; Liposomes–mycobacteria incubation systems as a partial model of host–parasite interaction at cell membrane level. Japanese Journal of Medical Science and Biology 38:169–180
    [Google Scholar]
  8. Wheeler P. R. 1984; Oxidation of carbon sources through the tricarboxylic acid cycle in Mycobacterium leprae grown in armadillo liver. Journal of Microbiology 130:381–389
    [Google Scholar]
  9. Wheeler P. R. 1987; Biosynthesis and scavenging of purines by pathogenic mycobacteria including Mycobacterium leprae. Journal of General Microbiology 133:2999–3011
    [Google Scholar]
  10. Wheeler P. R., Ratledge C. 1991; Phospholipase activity of Mycobacterium leprae harvested from experimentally infected armadillo tissue. Infection and Immunity 59:2781–2789
    [Google Scholar]
  11. Wheeler P. R., Bharadwaj V. P., Gregory D. 1982; N-Acetyl- β-glucosaminidase, β-glucuronidase and acid phosphatase in Mycobacterium leprae. Journal of General Microbiology 128:1063–1071
    [Google Scholar]
  12. Wheeler P., R” Bulmer K., Ratledge C. 1990; Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria: is Mycobacterium leprae competent in fatty acid biosynthesis?. Journal of General Microbiology 136:211–217
    [Google Scholar]
  13. World Health Organization 1980; UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Report of the Fifth Meeting on the Immunology of Leprosy (IMMLEP) TDR/IMMLEP-SWG (5)180.3, Annex 4, p. 23 Geneva: World Health Organization;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-4-825
Loading
/content/journal/micro/10.1099/00221287-138-4-825
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error