1887

Abstract

Summary: Pyruvate decarboxylase from the obligate anaerobe was purified eightfold. The subunit was 57000 ± 3000 as estimated from SDS-PAGE, and the native estimated by gel filtration on a Superose 6 column was 240000, indicating that the enzyme is a tetramer. The values are comparable to those for pyruvate decarboxylase from and , which are also tetrameric enzymes. The enzyme was oxygen stable, and had a pH optimum within the range 6·3-6·7. It displayed sigmoidal kinetics for pyruvate, with a of 13 m, kinetic properties also found for pyruvate decarboxylase from yeast and differing from the Michaelis-Menten kinetics of the enzyme from No activators were found. -Chloromercuri-benzoate inhibited activity and the inhibition was reversed by the addition of dithiothreitol, indicating that cysteine is important in the active site. The N-terminal amino acid sequence of pyruvate decarboxylase was more similar to the sequence of than pyruvate decarboxylase.

Funding
This study was supported by the:
  • National Science Foundation and Technology Center (Award DE-FG02- 87ER-13719)
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1992-04-01
2021-10-19
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