%0 Journal Article %A Mckay, David B. %A Jennings, Michael P. %A Godfrey, Elizabeth A. %A Macrae, Ian C. %A Rogers, Peter J. %A Beacham, Ifor R. %T Molecular analysis of an esterase-encoding gene from a lipolytic psychrotrophic pseudomonad %D 1992 %J Microbiology, %V 138 %N 4 %P 701-708 %@ 1465-2080 %R https://doi.org/10.1099/00221287-138-4-701 %I Microbiology Society, %X Summary: An esterase gene (estA) from a lipolytic psychrotroph (Pseudomonas sp. LS107d2), was cloned in Escherichia coli and its nucleotide sequence was determined, revealing an ORF encoding a polypeptide of 389 amino acid residues, with a molecular mass of 42276 Da. Labelling of plasmid-encoded proteins with [35S)methionine, using the maxicell procedure, gave a single polypeptide of molecular mass 42 kDa, consistent with that calculated from the ORF. Colonies of E. coli cells containing estA produced a clear halo when grown on solid media containing tributyrin; no clearance was produced when cells were grown on media containing triolein. Extracts of cells containing estA also hydrolysed water-soluble nitrophenol esters, but were unable to cleave water-insoluble substrates. The preference for water-soluble substrates indicates that the gene product is an esterase. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-4-701