1887

Abstract

Summary: The gene () encoding phosphoenolpyruvate carboxylase (PEPCase) in the cyanobacterium sp. PCC 7120 has been isolated, characterized and its nucleotide sequence determined. Heterologous hybridization using the sp. PCC 7942 gene as a probe of an genomic DNA library identified an 8·2 kb III DNA fragment that contained a 3·08 kb open reading frame encoding the cyanobacterial PEPCase. Deletion analysis of the 8·2 kb DNA fragment was used to determine sequences required for expression of enzyme activity in cells. Primer extension data have been used to identify the cyanobacterial transcription initiation site and the position of the start codon. Comparisons of the deduced amino acid sequence with the sp. PCC 6301, and higher-plant sequences have also been performed and the data are discussed with respect to conservation of specific regions of the protein.

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/content/journal/micro/10.1099/00221287-138-4-685
1992-04-01
2022-01-25
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References

  1. ALLEN M. M. . 1968; Simple conditions for growth of unicellular blue-green algae on plates. Journal of Phycology 4:1–4
    [Google Scholar]
  2. BORTHAKUR D. , HASELKORN R. . 1989; Nucleotide sequence of the gene encoding the 33 kDa water oxidizing polypeptide in Anabaena sp. strain PCC 7120 and its expression in Escherichia coli. Plant Molecular Biology 13:427–439
    [Google Scholar]
  3. BRADFORD M. M. . 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. COLEMAN J. R. . 1991; The molecular and biochemical analyses of CO2-concentrating mechanisms in cyanobacteria and rnicroalgae. Plant, Cell and Environment 14:861–867
    [Google Scholar]
  5. COLEMAN J. R. , COLMAN B. . 1980; Demonstration of C3 photosynthesis in the blue-green alga, Coccochloris peniocystis. Planta 149:318–320
    [Google Scholar]
  6. COLEMAN J. R. , COLMAN B. . 1981; Photosynthetic carbon assimilation in the blue-green alga, Coccochloris peniocystis. Plant, Cell and Environment 4:285–290
    [Google Scholar]
  7. COLMAN B. , CHENG K. H. , INGLE R. K. . 1976; The relative activities of PEP carboxylase and RuDP carboxylase in blue-green algae. Plant Science Letters 6:123–127
    [Google Scholar]
  8. CORPET F. . 1988; Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16:10881–10890
    [Google Scholar]
  9. CUSHMAN J. C. , MEYER G. , MICHALOWSKI C. B. , SCHMITT J. M. , BOHNERT H. J. . 1989; Salt stress leads to differential expression of two isogenes of phosphoenolpyruvate carboxylase during crassulacean acid metabolism induction in the common ice plant. The Plant Cell 1:715–725
    [Google Scholar]
  10. DOHLER G. . 1974; C-Weg der photosynthèse in der blaualga Anacystis nidulans. Planta 118:259–269
    [Google Scholar]
  11. FUJITA N. , MIWA T. , ISHIJIMA S. , IZUI K. , KATSUKI H. . 1984; The primary structure of phosphoenolpyruvate carboxylase of Escherichia coli. Nucleotide sequence of the ppc gene and deduced amino acid sequence. Journal of Biochemistry 95:909–916
    [Google Scholar]
  12. GENDEL S. , STRAUS N. , PULLEYBLANK D. , WILLIAMS J. . 1983; Shuttle cloning vectors for the cyanobacterium, Anacystis nidulans. Journal of Bacteriology 156:148–154
    [Google Scholar]
  13. GLANSDORFF N. . 1965; Topography of cotransducible arginine mutations in Escherichia coli K-l2. Genetics 51:167–179
    [Google Scholar]
  14. GOLDEN S. S. , BRUSSLAN J. , HASELKORN R. . 1987; Genetic engineering of the cyanobacterial chromosome. Methods in Enzymology 153:215–231
    [Google Scholar]
  15. INOUE M. , HAYASHI M. , SUGIMOTO M. , HARADA S. , KAI Y. , KASAI N. , TERADA K. , IZUI K. . 1989; First crystallization of a phosphoenolpyruvate carboxylase from Escherichia coli. Journal of Molecular Biology 208:509–510
    [Google Scholar]
  16. ISHIJIMA S. , KATAGIRI F. , KODAKI T. , IZUI K. , KATSUKI H. , NISHIKAWA K. , NAKASHIMA H. , OOI T. . 1985; Comparison of amino acid sequences between phosphoenolpyruvate carboxylases from Escherichia coli (allosteric) and Anacystis nidulans (non-allosteric): identification of conserved and variable regions. Biochemical and Biophysical Research Communications 133:436–441
    [Google Scholar]
  17. IZUI K. , ISHIJIMA S. , YAMAGUCHI Y. , KATAGIRI F. , MURATA T. , SHIGESADA K. , SUGIYAMA T. , HATSUKI H. . 1986; Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize. Nucleic Acids Research 14:1615–1628
    [Google Scholar]
  18. JIAO J. , PODESTA F. E. , CHOLLET R. ,, O' LEARY M. H. , ANDREO C. S. . 1990; Isolation and sequence of an active-site peptide from maize leaf phophoeno/pyruvate carboxylase inactivated by pyridoxal 5′-phosphate. Biochimica et Biophysica Acta 1041:291–295
    [Google Scholar]
  19. KATAGIRI F. , KODAKI T. , FUJITA N. , IZUI K. , KATSUKI H. . 1985; Nucleotide sequence of the phosphoenolpyruvate carboxylase gene of the cyanobacterium Anacystis nidulans. Gene 38:265–269
    [Google Scholar]
  20. KJEMS J. , GARRETT R. A. . 1988; Novel splicing mechanism for the ribosomal RNA intron in the archaebacterium Desulfurococcus mobilis. Cell 54:693–703
    [Google Scholar]
  21. KODAKI T. , KATAGIRI F. , ASANO M. , IZUI K. , KATSUKI H. . 1985; Cloning of phosphoenolpyruvate carboxylase gene from a cyanobacterium, Anacystis nidulans, in Escherichia coli. Journal of Biochemistry 97:533–539
    [Google Scholar]
  22. LEM N. W. , PENROSE D. M. , GLICK B. . 1986; Partial purification and characterization of phosphoenolpyruvate carboxylase from the cyanobacterium Anabaena variabilis. Biochemistry and Cell Biology 64:427–433
    [Google Scholar]
  23. LUINENBURG I. , COLEMAN J. R. . 1990; A requirement for phosphoenolpyruvate carboxylase in the cyanobacterium Synechococcus PCC 7942. Archives of Microbiology 154:471–474
    [Google Scholar]
  24. MARUYAMA H. , EASTERDAY R. L. , CHANG H. C. , LANE M. D. . 1966; The enzymatic carboxylation of phosphoenolpyruvate carboxylase. I. Purification and properties of phosphoenolpyruvate carboxylase. Journal of Biological Chemistry 241:2405–2412
    [Google Scholar]
  25. O'LEARY M. H. . 1982; Phosphoenolpyruvate carboxylase: an enzymologist's view. Annual Review of Plant Physiology 33:297–315
    [Google Scholar]
  26. OWTTRIM G. W. , COLEMAN J. R. . 1989; Regulation of expression and nucleotide sequence of the Anabaena variabilis recA gene. Journal of Bacteriology 171:5713–5719
    [Google Scholar]
  27. OWTTRIM G. W. , COLMAN B. . 1986; Purification and characterization of phosphoenolpyruvate carboxylase from a cyanobacterium. Journal of Bacteriology 168:207–212
    [Google Scholar]
  28. OWTTRIM G. W. , COLMAN B. . 1988; Phosphoenolpyruvate carboxylase mediated carbon flow in a cyanobacterium. Biochemistry and Cell Biology 66:93–99
    [Google Scholar]
  29. RICKERS J. , CUSHMAN J. C. , MICHALOWSKI C. B. , SCHMITT J. M. , BOHNERT H. J. . 1989; Expression of the CAM-form of phospho-(enol)pyruvate carboxylase and nucleotide sequence of a full length cDNA from Mesembryanthemum crystallinum. Molecular and General Genetics 215:447–454
    [Google Scholar]
  30. SAMBROOK J. , FRITSCH E. F. , MANIATIS T. . 1989 Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. SCHNEIDER G. J. , LANG J. D. , HASELKORN R. . 1991; Promoter recognition by the RNA polymerase from the vegetative cells of the cyanobacterium Anabaena 7120. Gene 105:51–60
    [Google Scholar]
  32. SMITH A. J. , LONDON J. , STANIER R. Y. . 1967; Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. Journal of Bacteriology 94:972–983
    [Google Scholar]
  33. SUTTON F. , BUTLER E. T., III , SMITH T. E. . 1986; Isolation of the structural gene encoding a mutant form of Escherichia coli phosphoenolpyruvate carboxylase deficient in regulation by fructose 1,6-bisphosphate. Identification of an amino acid substitution in the mutant. Journal of Biological Chemistry 261:16078–16081
    [Google Scholar]
  34. TANDEAU DE MARSAC N. , HOUMARD J. . 1987 Advances in cyanobacterial molecular genetics. The Cyanobacteria251–302 P. Fay , C. van Baalen . Amsterdam: Elsevier;
    [Google Scholar]
  35. TERADA K. , MURATA T. , IZUI K. . 1991; Site directed mutagenesis of phosphoenolpyruvate carboxylase from E. coli: The role of His579 in the catalytic and regulatory functions. Journal of Biochemistry 109:49–54
    [Google Scholar]
  36. TUMER N. E. , ROBINSON S. J. , HASELKORN R. . 1983; Different promoters for the Anabaena glutamine synthetase gene during growth using molecular or fixed nitrogen. Nature, London 306:337–342
    [Google Scholar]
  37. WEATHERS P. J. , ALLEN M. M. . 1978; Variations in short-term products of inorganic carbon fixation in exponential and stationary phase cultures of Aphanocapsa 6308. Archives of Microbiology 116:231–234
    [Google Scholar]
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