1887

Abstract

Summary: The growth and survival of two genetically engineered micro-organisms (GEMs), strain PaW8 containing either marker plasmid pLV1013 (IncQ) or pLV1016 (IncP), both of which contain the gene, were investigated in soils ranging in texture from a sandy loam to a peat. Viable GEMs were enumerated over a period of 89 d. Analysis of the decay kinetics indicated that GEM survival, as monitored by the persistence of the gene, was biphasic in the sandy and clay loams. Both organisms showed evidence of initial growth in the peat soil prior to a rapid death phase. The results indicated that soil texture has an effect on the survival of genetically engineered , death decreasing with increasing clay content.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-4-667
1992-04-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/4/mic-138-4-667.html?itemId=/content/journal/micro/10.1099/00221287-138-4-667&mimeType=html&fmt=ahah

References

  1. ALEXANDER M. . 1981; Why microbial predators and parasites do not eliminate their prey and hosts. Annual Review of Microbiology 35:113–133
    [Google Scholar]
  2. BLACK C. A. . 1965 Methods of Soil Analysis: Physical and Mineralogical Properties. Madison, WI: American Society of Agronomy;
    [Google Scholar]
  3. CHAO W. L. , ALEXANDER M. . 1981; Interaction between protozoa and Rhizobium in chemically amended soil. Soil Science Society of America Journal 45:48–50
    [Google Scholar]
  4. CLARHOLM M. . 1981; Protozoan grazing of bacteria – impact and importance. Microbial Ecology 7:343–350
    [Google Scholar]
  5. CROMPTON E. . 1966 The soils of the Preston district of Lancashire. Soil Survey of Great Britain. Harpenden: Rothamsted Experimental Station;
    [Google Scholar]
  6. DANSO S. K. A. , KEYA S. O. , ALEXANDER M. . 1975; Protozoa and the decline of Rhizobium populations added to soil. Canadian Journal of Microbiology 21:884–895
    [Google Scholar]
  7. DOMSCH K. H. , DRIESEL A. J. , GOEBEL W. , ANDERSCH W. , LINDENMAIER W. , LOTZ W. , REBER H. , SCHMIDT F. . 1988; Considerations on release of gene-technologically engineered microorganisms into the environment. FEMS Microbiology Ecology 53:261–272
    [Google Scholar]
  8. VAN ELSAS J. D. , DIJKSTRA A. F. , GOVAERT J. M. , VAN VEEN J. A. . 1986; Survival of Pseudomonas fluorescens and Bacillus subtilis introduced into two soils of different texture in field microplots. FEMS Microbiology Ecology 38:151–160
    [Google Scholar]
  9. VAN ELSAS J. D. , TREVORS J. T. , STARODUB M. E. , VAN OVERBEEK L. S. . 1990; Transfer of plasmid RP4 between pseudomonads after introduction into soil; influence of spatial and temporal aspects of inoculation. FEMS Microbiology Ecology 73:1–12
    [Google Scholar]
  10. VAN ELSAS J. D. , VAN OVERBEEK L. S. , FELDMANN A. M. , DULLEMANS A. M. , DE LEEUW O. . 1991; Survival of genetically engineered Pseudomonas fluorescens in soil in competition with the parent strain. FEMS Microbiology Ecology 85:53–64
    [Google Scholar]
  11. HABTE M. , ALEXANDER M. . 1978; Mechanisms of persistence of low numbers of bacteria preyed upon by protozoa. Soil Biology and Biochemistry 10:1–6
    [Google Scholar]
  12. HEYNEN C. E. , VAN ELSAS J. D. , KUIKMAN P. J. , VAN VEEN J. A. . 1988; Dynamics of Rhizobium leguminosarum biovar trifolii introduced into soil; the effect of bentonite clayon predation by protozoa. Soil Biology and Biochemistry 20:483–488
    [Google Scholar]
  13. HOPKINS D.W.H. , MACNAUGHTON S. J. , & O' DONNELL A. G. . 1991; A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil. Soil Biology and Biochemistry 23:217–225
    [Google Scholar]
  14. MARSHALL K. C. , ROBERTS F. J. . 1963; Influence of fine particles on survival of Rhizobium trifolii in sandy soil. Nature, London 198:410–411
    [Google Scholar]
  15. MORGAN J. A. W. , WINSTANLEY C. , PICKUP R. W. , JONES J. G. , SAUNDERS J. R. . 1989; Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. Applied and Environmental Microbiology 55:2537–2544
    [Google Scholar]
  16. ORVOS D. R. , LACY G. H. , CAIRNS J., JR . 1990; Genetically engineered Erwinia carotovora: survival, interspecific competition and effects upon selected bacterial genera. Applied and Environmental Microbiology 56:1689–1694
    [Google Scholar]
  17. RICHAUME A. , ANGLE J. S. , SADOWSKY J. . 1989; Influence of soil variables in in situ plasmid transfer from Escherichia coli to Rhizobium fredii. Applied and Environmental Microbiology 55:1730–1734
    [Google Scholar]
  18. ROSE D. A. . 1990; Testing goodness-of-fit. Journal of Agricultural Science 117:132–133
    [Google Scholar]
  19. STOTZKY G. . 1980 Surface interactions between clay minerals and microbes, viruses and soluble organics, and the probable importance of these interactions to the ecology of the soil. Microbial Adhesion to Surfaces231–447 R. C. Berkeley , J. M. Lynch , J. Melling , P. R. Rutter , B. Vincent . Chichester: Ellis Horwood;
    [Google Scholar]
  20. STOTZKY G. . 1986 Influence of soil mineral colloids on metabolic processes, growth, adhesion and ecology of microbes and viruses. Interactions of Soil Minerals with Natural Organics and Microbes305–428 P. M. Haung , M. Schnitzer . Madison, WI: Soil Science Society of America;
    [Google Scholar]
  21. TIEDJE J. M. , COLWELL R. K. , GROSSMAN Y. L. , HODSON R. E. , LENSKI R. E. , MACK R. N. , REGAL P. J. . 1989; The planned introduction of genetically engineered organisms: ecological considerations and recommendations. Ecology 70:298–315
    [Google Scholar]
  22. WINSTANLEY C. , MORGAN J. A. W. , PICKUP R. W. , JONES J. G. , SAUNDERS J. R. . 1989; Differential regulation of lambda P1 and PR promoters by a repressor in a broad-host range thermoregulated plasmid marker system. Applied and Environmental Microbiology 55:771–777
    [Google Scholar]
  23. WINSTANLEY C. , MORGAN J. A. W. , PICKUP R. W. , SAUNDERS J. R. . 1991; Use of a xylE marker gene to monitor survival of recombinant Pseudomonas putida populations in lake water by culture on nonselective media. Applied and Environmental Microbiology 57:1905–1913
    [Google Scholar]
  24. YEUNG KAM-HA ANNA , SCHELL M. A. , HARTEL P. G. . 1989; Growth of genetically engineered Pseudomonas aeruginosa and Pseudomonas putida in soil and rhizosphere. Applied and Environmental Microbiology 55:3243–3246
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-4-667
Loading
/content/journal/micro/10.1099/00221287-138-4-667
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error