1887

Abstract

Summary: Populations of and were monitored in natural soil amended with nutrients. The fates of a multicopy plasmid pIJ673, and an actinophage, KC301, were determined and the extent of gene transfer was estimated. The soil was incubated for 60 d during which time ‘spent’ soil was periodically removed followed by addition of fresh, uninoculated soil. Maximum numbers of bacteria and phage inoculants occurred at 15 d; this correlated with a peak in the amount of plasmid DNA detected and total numbers of transconjugants recovered. A KC301 lysogen of was also recovered at this time. Plasmid DNA was monitored by two methods, bead-beating and SDS/heat lysis; the latter was specific for mycelium while the former released DNA from spores and mycelium. Southern blots of soil DNA only showed the presence of plasmid DNA in SDS/heat lysis extracts from 15 d and 17 d samples, whereas positive signals were obtained throughout the experiment from bead-beaten extracts. The results confirmed that a well-developed mycelium was necessary for conjugation, phage infection, multiplication and lysogeny in soil.

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1992-04-01
2022-01-23
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References

  1. BLEAKLEY B. H. , CRAWFORD D. L. . 1989; The effects of varying moisture and nutrient levels on the transfer of a conjugative plasmid between Streptomyces species in soil. Canadian Journal of Microbiology 35:544–549
    [Google Scholar]
  2. CHATER K. F. . 1986 Streptomyces phage and their applications to Streptomyces genetics. The Bacteria, vol. IX: The Antibiotic Producing Streptomyces119–158 S. W. Queener , L. E. Day . Orlando: Academic Press;
    [Google Scholar]
  3. CLEWLOW L. J. , CRESSWELL N. , WELLINGTON E. M. H. . 1990; Mathematical model of plasmid transfer between strains of streptomycetes in soil microcosms. Applied and Environmental Microbiology 56:3139–3145
    [Google Scholar]
  4. CRESSWELL N. , WELLINGTON E. M. H. , SAUNDERS V. A. . 1991; Detection and quantification of Streptomyces violaceolatus plasmid DNA in soil. Letters in Applied Microbiology 13:193–197
    [Google Scholar]
  5. FRY J. C. . 1989; Analysis of variance and regression in aquatic bacteriology. Binary 1:83–88
    [Google Scholar]
  6. GARRETTE A. , WITTMAN H. G. . 1974; Structure of bacterial ribosomes. Advances in Protein Chemistry 27:277–347
    [Google Scholar]
  7. GERMIDA J. J. . 1986; Population dynamics of Azospirillum brasilense and its bacteriophage in soil. Plant and Soil 90:117–128
    [Google Scholar]
  8. HERRON P. R. . 1991 Interactions between actinophage and streptomycetes in soil. PhD. thesis University of Warwick;
    [Google Scholar]
  9. HERRON P. R. , WELLINGTON E. M. H. . 1990; New method for extraction of streptomycete spores from soil and application to the study of lysogeny in sterile and nonsterile soil. Applied and Environmental Microbiology 56:1406–1412
    [Google Scholar]
  10. HOPWOOD D. A. , BIBB M. J. , CHATER K. F. , KIESER T. , BRUTON C. J. , KIESER H. M. , LYDIATE D. J. , SMITH C. P. , WARD J. M. , SCHREMPF H. . 1985 Genetic Manipulation of Streptomyces - a Laboratory Manual. Norwich: The John Innes Institute;
    [Google Scholar]
  11. KIESER T. , HOPWOOD D. A. , WRIGHT H. M. , THOMPSON C. J. . 1982; pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Molecular and General Genetics 185:223–238
    [Google Scholar]
  12. LANNING S. , WILLIAMS S. T. . 1982; Methods for the direct isolation and enumeration of actinophage in soil. Journal of General Microbiology 128:2063–2071
    [Google Scholar]
  13. LOMOVSKAYA N. D. , MKRTUMIAN N. M. , GOSTIMAYA N. L. , DANILENKO V. N. . 1972; Characterisation of the temperate actinophage ϕC31 isolated from Streptomyces coelicolor A3(2). Journal of Virology 9:258–262
    [Google Scholar]
  14. LOMOVSKAYA N. D. , CHATER K. F. , MKRTUMIAN N. M. . 1980; Genetics and molecular biology of Streptomyces bacteriophage. Microbiological Reviews 44:206–229
    [Google Scholar]
  15. MANCHESTER L. . 1986 Modelling the interactions of streptomycetes and their phage. PhD thesis University of Manchester;
    [Google Scholar]
  16. NEDWELL D. B. , GRAY T. R. G. . 1987 Soils and sediments as matrices for microbial growth. Ecology of Microbial Communities21–54 M. Fletcher , T. R. G. Gray , J. G. Jones . Cambridge: Cambridge University Press;
    [Google Scholar]
  17. OGRAM A. , SAYLER G. S. , BARKAY T. . 1987; The extraction and purification of microbial DNA from sediments. Journal of Microbiological Methods 7:57–66
    [Google Scholar]
  18. PETERSEN R. G. . 1985 Separation of means. Design and Analysis of Experiments Statistics: Textbooks and Monographs 6672–111 New York: Marcel Dekker;
    [Google Scholar]
  19. PICKUP R. W. . 1991; Development of molecular methods for the detection of specific bacteria in the environment. Journal of General Microbiology 137:1009–1019
    [Google Scholar]
  20. RAFII F. , CRAWFORD D. L. . 1988; Transfer of conjugative plasmids and mobilisation of a non-conjugative plasmid between Streptomyces strains on agar and in soil. Applied and Environmental Microbiology 54:1334–1340
    [Google Scholar]
  21. RAFII F. , CRAWFORD D. L. . 1989; Donor/recipient interactions affecting plasmid transfer among Streptomyces species: a conjugative plasmid will mobilise non-transferable plasmids in soil. Current Microbiology 19:115–121
    [Google Scholar]
  22. RYAN B. F. , JOINER B. L. , RYAN T. A., JR . 1985 Minitab Handbook, 2nd edn. Boston: PWS-Kent;
    [Google Scholar]
  23. SELENSKA S. , KLINGMÜLLER W. . 1991a; DNA recovery and direct detection of Tn5 sequences from soil. Letters in Applied Microbiology 13:21–24
    [Google Scholar]
  24. SELENSKA S. , KLINGMÜLLER W. . 1991b; Direct detection of nif-gene sequences of Enterobacter agglomerans in soil. FEMS Microbiology Letters 80:243–246
    [Google Scholar]
  25. STOTZKY G. , BABICH H. . 1986; Survival of, and genetic transfer by, genetically engineered bacteria in natural environments. Advances in Applied Microbiology 31:93–138
    [Google Scholar]
  26. STOTZKY G. , DEVANAS M. A. , ZEPH L. R. . 1990; Methods for studying bacterial gene transfer in soil by conjugation and transduction. Advances in Applied Microbiology 35:57–169
    [Google Scholar]
  27. STUTTARD C. . 1979; Transduction of auxotrophic markers in a chloramphenicol producing strain of Streptomyces. Journal of General Microbiology 110:479–482
    [Google Scholar]
  28. WANG Z. , CRAWFORD D. L. , POMETTO A. L. , RAFII F. . 1989; Survival and effects of wild type, mutant and recombinant Streptomyces in a soil ecosystem. Canadian Journal of Microbiology 35:535–543
    [Google Scholar]
  29. WELLINGTON E. M. H. , SAUNDERS V. A. , CRESSWELL N. , WIPAT A. . 1988 Plasmid transfer between streptomycetes in soil. Biology of Actinomycetes301–305 Y. Okami , T. Beppu , H. Ogawara . Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  30. WELLINGTON E. M. H. , CRESSWELL N. , SAUNDERS V. A. . 1990a; Growth and survival of streptomycete inoculants in sterile and nonsterile soil. Applied and Environmental Microbiology 56:1413–1419
    [Google Scholar]
  31. WELLINGTON E. M. H. , CRESSWELL N. , HERRON P. R. , CLEWLOW L. J. , SAUNDERS V. A. , WIPAT A. . 1990b Gene transfer between streptomycetes in soil. Bacterial Genetics in Natural Environments216–230 J. C. Fry , M. J. Day . London: Chapman & Hall;
    [Google Scholar]
  32. WILLIAMS S. T. , MORTIMER A. M. , MANCHESTER L. . 1986 The ecology of soil bacteriophage. Phage Ecology157–180 S. M. Goyal , C. P. Gerba , G. Bitton . New York: John Wiley;
    [Google Scholar]
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