1887

Abstract

Summary: Copper-zinc superoxide dismutases ([Cu,Zn]-SODs) are ubiquitous in eukaryotes but have rarely been found in prokaryotes. A gene for [Cu,Zn]-SOD () has recently been cloned from type b and , so other and related species were screened for the presence of [Cu,Zn[-SODs by visualization of bands of SOD activity in non-denaturing polyacrylamide gels and by gene probing. Strains of , some non-typable () and were all found to have [Cu,Zn]-SOD activity (inhibited by 2 m-cyanide) in polyacrylamide gels. In a Southern blot analysis, DNA from and [Cu,Zn]-SOD-containing non-typable - but not the other species - hybridized to a 360 nucleotide DNA probe containing the 5′-part of cloned from type b. Bacterial [Cu,Zn]-SODs are more prevalent than has previously been recognized.

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/content/journal/micro/10.1099/00221287-138-3-517
1992-03-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-3-517
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