1887

Abstract

Summary: Under anaerobic 2-ketogluconate-limited growth conditions ( = 0·1 h), NCTC 418 was found to convert this carbon source to biomass, acetate, formate, CO, ethanol and succinate. The observed fermentation pattern is in agreement with the simultaneous functioning of the pentose phosphate pathway and the Entner-Doudoroff pathway in 2-ketogluconate catabolism. When cultured at pH 8·0 apparent values were lower than those found at culture pH 6·5. This difference can be explained by assuming that at high culture pH values approximately 0·5 mol ATP was invested in the uptake of 1 mol 2-ketogluconate. Sudden relief of 2-ketogluconate-limited conditions led to lowering of the intracellular NADPH/NADP ratio and (possibly as a result of this) to inhibition of biosynthesis. Whereas production of ethanol stopped, lactate was produced at high rate. This product was formed, at least partly, via the methylglyoxal bypass.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-3-423
1992-03-01
2024-12-08
Loading full text...

Full text loading...

/content/journal/micro/10.1099/00221287-138-3-423
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error