@article{mbs:/content/journal/micro/10.1099/00221287-138-2-369, author = "Pedersen, Karsten and Ekendahl, Susanne", title = "Incorporation of CO2 and introduced organic compounds by bacterial populations in groundwater from the deep crystalline bedrock of the Stripa mine", journal= "Microbiology", year = "1992", volume = "138", number = "2", pages = "369-376", doi = "https://doi.org/10.1099/00221287-138-2-369", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-2-369", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The incorporation of CO2 and assimilation of introduced organic compounds by bacterial populations in deep groundwater from fractured crystalline bedrock has been studied. Three depth horizons of the subvertical borehole V2 in the Stripa mine, Sweden, 799-807 m, 812-820 m and 970-1240 m, were sampled. The groundwaters, obtained from fracture systems without close hydraulic connections, were anoxic and had the following physicochemical characteristics: pH values of 9·5, 9·4 and 10·2; E h values of + 205, + 199 and—3 mV; sulphide, 0, 106 and 233 μM; CO2- 3, 158, 50 and 57 μm; CH4, 245, 170 and 290 μl l-1; and N2, 25, 31 and 25 ml l-1. Biofilm reactors, each containing a series of parallel glass surfaces, were connected to the groundwaters issuing from these depth horizons at flows of approximately 1×10-3 m s-1 during two periods of two and four months. There were from 1·8×103 to 1·2×105 bacteria per ml groundwater and from 1·2×106 to 7·1×106 bacteria per cm2 of colonized test surface. These results imply that the populations of attached bacteria are several orders of magnitude greater than those of unattached bacteria in bedrock fractures with flowing groundwater. The incorporation of 14CO2, [14C]formate, [U-14C]lactate, [U-14C]glucose and l-[4,5-3H]leucine by the bacterial populations was demonstrated using microautoradiographic and liquid scintillation counting techniques. The measured CO2 incorporation reflected the in situ production of organic carbon from CO2. Incorporation of formate followed that of CO2 and indicated the presence of bacteria able to substitute formate for CO2, e.g. methanogenic bacteria. The presence of sulphate-reducing bacteria is suggested by the observed incorporation of lactate by up to 74% of the bacterial populations. The recorded uptake of glucose indicates the presence of heterotrophic bacteria other than sulphate-reducing bacteria. Up to 99% of the populations incorporated leucine, showing that major fractions of the populations were viable.", }