1887

Abstract

SUMMARY: Addition of the amino acid analogue -7-azatryptophan (AT) to dinitrogen-fixing cultures of the cyanobacterium , and to cultures in which heterocyst development was induced by the removal of fixed nitrogen from the medium, resulted in the development of many adjacent (double) heterocysts. Cell division in was asymmetrical, with a maximum of 10% of all cell divisions producing two daughter cells of equal size. During incubation with AT the frequency of symmetrical cell divisions remained unchanged, indicating that the preponderance of double heterocysts induced by the analogue did not result from any change in the symmetry of cell division. Incubation of cultures with AT resulted in a decrease in the number of vegetative cells between heterocysts (the interheterocyst interval). The extent of the decrease was proportional to the length of the incubation period in the presence of AT. Double heterocysts, which constitute a zero interval, developed at a time when the minimum interval was three cells in dinitrogen-fixing cultures, or nine cells in cultures induced to differentiate by the removal of fixed nitrogen from the medium. These observations have been used to formulate a model to explain the influence of AT on the control of heterocyst development and spacing. In this model the inactive form of an inhibitor of heterocyst development is produced constitutively by vegetative cells and is activated either by a co-inhibitor derived from developing or mature heterocysts, or by high concentrations of fixed nitrogen.

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/content/journal/micro/10.1099/00221287-138-2-355
1992-02-01
2021-04-21
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References

  1. Adams D. G. 1978 Differentiation, pattern control and macromolecule synthesis in the blue-green alga Anabaena cylindrica.PhD thesis University of Liverpool:
  2. Adams D. G., Carr N. G. 1981a; The developmental biology of heterocyst and akinete formation in cyanobacteria. CRC Critical Reviews in Microbiology 9:45–100
    [Google Scholar]
  3. Adams D. G., Carr N. G. 1981b; Heterocyst differentiation and cell division in the cyanobacterium Anabaena cylindrica: effect of high light intensity. Journal of Cell Science 49:341–352
    [Google Scholar]
  4. Adams D. G., Carr N. G. 1989; Control of heterocyst development in the cyanobacterium Anabaena cylindrica. Journal of General Microbiology 135:839–849
    [Google Scholar]
  5. Allen M. B., Arnon D. I. 1955; Studies on nitrogen-fixing blue-green algae. Plant Physiology 30:366–372
    [Google Scholar]
  6. Bothe H., Eisbrenner G. 1977; Effect of 7-azatryptophan on nitrogen fixation and heterocyst formation in the blue-green alga Anabaena cylindrica. Biochemie und Physiologie der Pflanzen 171:323–332
    [Google Scholar]
  7. Bottomley P. J., Van Baalen C., Tabita F. R. 1980; Heterocyst differentiation and tryptophan metabolism in the cyanobacterium Anabaena sp. CA. Archives of Biochemistry and Biophysics 203:204–213
    [Google Scholar]
  8. Chen C, Van Baalen C., Tabita F. R. 1987; Nitrogen starvation mediated by dl-7-azatryptophan in the cyanobacterium Anabaena sp. strain CA. Journal of Bacteriology 169:1107–1113
    [Google Scholar]
  9. Fogg G. E. 1944; Growth and heterocyst production in Anabaena cylindrica Lemm. New Phytologist 43:164–175
    [Google Scholar]
  10. Fogg G. E. 1949; Growth and heterocyst production in Anabaena cylindrica Lemm. II. In relation to carbon and nitrogen metabolism. Annals of Botany NS 13:241–259
    [Google Scholar]
  11. Kumar A., Kumar H. D. 1980; Differential effects of amino acid analogs on growth and heterocyst differentiation in two nitrogen-fixing blue-green algae. Current Microbiology 3:213–218
    [Google Scholar]
  12. Mitchison G. J., Wilcox M. 1972; Rule governing cell division in Anabaena. Nature, London 239:110–111
    [Google Scholar]
  13. Mitchison G. J., Wilcox M. 1973; Alteration in heterocyst pattern of Anabaena produced by 7-azatryptophan. Nature New Biology 246:229–233
    [Google Scholar]
  14. Rogerson A. C. 1979; Modifiers of heterocyst repression and spacing and formation of heterocysts without nitrogenase in the cyanobacterium Anabaena variabilis. Journal of Bacteriology 140:213–219
    [Google Scholar]
  15. Thiel T. 1990; Protein turnover and heterocyst differentiation in the cyanobacterium Anabaena variabilis. Journal of Phycology 26:50–54
    [Google Scholar]
  16. Thiel T., Leone M. 1986; Effect of glutamine on growth and heterocyst differentiation in the cyanobacterium Anabaena variabilis. Journal of Bacteriology 168:769–774
    [Google Scholar]
  17. Wilcox M., Mitchison G. J., Smith R. J. 1973; Pattern formation in the blue-green alga Anabaena. II. Controlled proheterocyst regression. Journal of Cell Science 13:637–649
    [Google Scholar]
  18. Wolk C. P. 1967; Physiological basis of the pattern of vegetative growth of a blue-green alga. Proceedings of the National Academy of Sciences of the United States of America 57:1246–1251
    [Google Scholar]
  19. Wolk C. P. 1975; Differentiation and pattern formation in filamentous blue-green algae. In Spores VI pp. 85–96 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Wolk C. P. 1989; Alternative models for the development of the pattern of spaced heterocysts in Anabaena (Cyanophyta). Plant Systematics and Evolution 164:27–31
    [Google Scholar]
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