1887

Abstract

SUMMARY: The group C1 O antigen structure has a Man-Man-Man-Man-GlcNAc backbone with a glucose branch, which differs from the group B O antigen structure which has a Man-Rha-Gal backbone with abequose as side-chain. We have cloned the group C1 (O antigen) gene cluster from serovar strain M40, using a low-copy-number cosmid vector. The restriction map of the group C1 (M40) gene cluster was compared with that of group B strain LT2 by Southern hybridization and restriction enzyme analysis. The results indicate that the flanking genes are very similar in the two strains, but there is no detectable similarity in the regions. We localized the mannose pathway genes and and one of the genes, , shows considerably similarity to of strain LT2, suggesting that part of the mannose pathway in the group C1 cluster is derived from a gene of the M antigen () cluster. The M antigen, which forms a capsule, is comprised of four sugars, including fucose. The biosynthetic pathway of GDP-fucose has steps in common with the GDP-mannose pathway, and the cluster has isogenes of and , presumably as part of a fucose pathway. We discuss the structure and possible evolution of the group C1 gene cluster.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-2-305
1992-02-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/2/mic-138-2-305.html?itemId=/content/journal/micro/10.1099/00221287-138-2-305&mimeType=html&fmt=ahah

References

  1. van alphen L., Verkleij A., Leunissen-Bijvelt J., Lugtenberg B. 1978; Architecture of the outer membrane of Escherichia coli. III. Protein-lipopolysaccharide complexes in intermembraneous particles. Journal of Bacteriology 134:1089–1098
    [Google Scholar]
  2. Beltz G. A., Jacobs K. A., Eickbush T. H., Cherbas P. T., Kafatos F. C. 1983; Isolation of multigene families and determination of homologies by filter hybridization methods. Methods in Enzymology 100:266–285
    [Google Scholar]
  3. Brahmbhatt H. N., Wyk P., Quigley N. B., Reeves P. R. 1988; Complete physical map of the rfb gene cluster encoding biosynthetic enzymes for the O antigen of Salmonella typhimurium LT2. Journal of Bacteriology 170:98–102
    [Google Scholar]
  4. Brenner D. J. 1978 Characterization and identification of Enterobacteriaceae by DNA hybridization. In Progress in Clinical Pathology, pp. 71–117 Edited by Stefanini M. New York: Grune & Stratton;
    [Google Scholar]
  5. Brown P. K., Romana L. K., Reeves P. R. 1991; Cloning of the rfb gene cluster of a group C2 Salmonella: comparison with the rfb regions of groups B and D. Molecular Microbiology 5:1873–1881
    [Google Scholar]
  6. Bullas L. R., Ryu J.-I. 1983; Salmonella typhimurium LT2 strains which are r-m+ for all three chromosomally located systems of DNA restriction and modification. Journal of Bacteriology 156:471–474
    [Google Scholar]
  7. Davis D. B., MlNGOLi E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamine B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  8. Ewing W. H. 1986 Edwards and Ewing's Identification of Enterobacteriaceae,, 4th edn. New York: Elsevier Science Publishers;
  9. Fuller N. A., Staub A. M. 1968; Immunochemical studies on Salmonella. European Journal of Biochemistry 4:286–300
    [Google Scholar]
  10. Heine H.-G., Francis G., Lee K.-S., Ferenci T. 1988; Genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure. Journal of Bacteriology 170:1730–1738
    [Google Scholar]
  11. Howley P. M., Israel M. A., Law M. F., Martin M. A. 1979; A rapid method for detecting and mapping homology between heterologous DNAs. Journal of Biological Chemistry 254:4876–4883
    [Google Scholar]
  12. Jacobs W. R., Barrett J. F., Clark-Curtiss J. E., Curtiss R. 1986; In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries. Infection and Immunity 52:101–109
    [Google Scholar]
  13. Jiang X.-M., Brahmbhatt H. N., Quigley N. B., Reeves P. R. 1987; A low copy number cosmid. Plasmid 18:170–172
    [Google Scholar]
  14. Jiang X.-M., Neal B., Santiago F., Lee S. J., Romana L. K., Reeves P. R. 1991 Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Molecular Microbiology 5695–713 Kauffmann F. 1966 Enterobacteriaceae. Copenhagen: Ejnar Munksgaad;
    [Google Scholar]
  15. Le Minor L., Popoff M. Y. 1987; Designation of Salmonella enterica sp. nov., nom. rev., as the type and only species of the genus Salmonella. International Journal of Systematic Bacteriology 37:465–468
    [Google Scholar]
  16. Leinonen M. 1985 Serological methods for the study of bacterial surface antigens. In Enterobacterial Surface Antigens : Methods for Molecular Characterisation, pp. 179–206 Edited by Korhonen T. K., Dawes E. A., Makela P. H. Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  17. Lindberg B., Leontein K., Lindquist U., Svenson S. B., Wrangsell G., Dell A., Rogers M. 1988; Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup CI (6,7). Carbohydrate Research 174:313–322
    [Google Scholar]
  18. Liu D., Verma N. K., Romana L. K. & Reeves P. R. 1991; Completing the sequence of the rfb region of Salmonella serovars typhi (group D) and paratyphi (group A), and relationships to group B. Journal of Bacteriology 173:4814–4819
    [Google Scholar]
  19. Lüderitz O., Jann K., Wheat R. 1968 Somatic and Capsular Antigens of Gram-Negative Bacteria. Amsterdam: Elsevier Publishing Company;
  20. Lugtenberg B., Meijers J., Peters R., van der hoek P., van Alphen L. 1975; Electrophoretic resolution of the major outer membrane proteins of Escherichia coli K12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  21. Mäkelä P. H., Stocker B. A. D. 1984 Genetics of lipopolysaccharide. In Handbook of Endotoxin, pp. 59–137 Edited by Rietschel E. T. Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  23. Munch-Petersen A. 1962; GDPM pyrophosphorylase. Methods in Enzymology 5:171–174
    [Google Scholar]
  24. Nasoff M. S., Wolf R. E. Jr 1980; Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli. Journal of Bacteriology 143:731–741
    [Google Scholar]
  25. Neuhard J., Thomassen E. 1976; Altered deoxyribonucleotide pools in P2 educants of Escherichia coli K-12 due to deletion of the dcd gene. Journal of Bacteriology 126:999–1001
    [Google Scholar]
  26. Nikaido H., Nikaido K., Makela P. H. 1966; Genetic determination of enzymes synthesizing O-specific sugars of Salmonella lipopolysaccharides. Journal of Bacteriology 91:1126–1135
    [Google Scholar]
  27. Ratcliff S. W., Luh J., Ganesan A. T., Behrens B., Thompson R., Montenegro M. A., Morelli G., Trautner T. A. 1979; The genome of Bacillus subtilis SPP1: the arrangement of restriction endonuclease generated fragments. Molecular and General Genetics 168:165–172
    [Google Scholar]
  28. Rosenberg S. M., Stahl M. M., Kobayashi I., Stahl F. W. 1985; Improved in vitro packaging of coliphage lambda DNA: a one-strain system free from endogenous phage. Gene 38:165–175
    [Google Scholar]
  29. Sanderson K. E., Hurley J. A. 1987 Linkage map of. Salmonella typhimurium. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, pp. 877–918 Edited by Neidhardt F. C., Ingraham J. L., Low K. B., Magasanik B., Schaechter M., Umbarger H. E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Sanderson K. E., Roth J. R. 1989; Linkage map of Salmonella typhimurium. Microbiological Reviews 52:485–532
    [Google Scholar]
  31. Stevenson G. S., Lee S. J., Romana L. K., Reeves P. R. 1991; The cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster. Molecular and General Genetics 227:173–180
    [Google Scholar]
  32. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
  33. Verma N. K., Reeves P. R. 1989; Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae. Journal of Bacteriology 171:5694–5701
    [Google Scholar]
  34. Verma N. K., Quigley N. B., Reeves P. R. 1988; O-Antigen variation in Salmonella spp.: rfb gene clusters of three strains. Journal of Bacteriology 170:103–107
    [Google Scholar]
  35. Vieira J., Messing J. 1982; The pUC plasmids, a M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  36. Wang L., Romana L. K., Reeves P. R. 1992; Molecular analysis of a Salmonella enterica group E1 rfb gene cluster: O antigen and the genetic basis of the major polymorphism. Genetics (in the Press)
    [Google Scholar]
  37. Wyk P., Reeves P. R. 1989; Identification and sequence of the gene for abequose synthase which confers antigenic specificity on group B salmonellae: homology with galactose epimerase. Journal of Bacteriology 171:5687–5693
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-2-305
Loading
/content/journal/micro/10.1099/00221287-138-2-305
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error